ABSTRACT
BACKGROUND: We aimed to research the value of extended nursing for cerebral stroke patients within a suitable recovery empty period. METHODS: Seventy-two cerebral stroke patients were randomized to a control group or treatment group at the recovery period at Xuzhou Recovery Hospital, China in 2016. A recovery guidance exercise was applied to the control group for a set time, while a recovery guidance exercise combined with functional training were applied to the treatment group within the recovery empty period (at 6:00-7:00 a.m. and 7:00-8:00 p.m.). The recovery effect was compared after three months. RESULTS: Following the three-month intervention, both the control and treatment groups' scores for the Fugl-Meyer balance evaluation and the Barthel indicator were increased. There was a statistically significant increase in the treatment group (P<0.05). Scores for the Self-Rating Depression Scale in both groups declined and the decline in the treatment group was statistically significant greater when compared to the control group (P<0.05). The total depression rate for the treatment group was significantly lower than the control group and the severe extent of depression in the treatment group was significantly less than the control group (P<0.05). Both groups' scores for the PSQI also decreased with a significantly greater increase in the treatment group (P<0.05). CONCLUSION: Extended nursing within a suitable recovery empty period can improve the patient's prognosis concerning physical activity and mood.
ABSTRACT
Objective Percoll density gradient centrifugation and Ficoll-Hypaque density gradient cen-trifugation, which are frequently-used methods for separation of tumor-associated macrophages (TAMs) from solid carcinoma were compared, in order to find an effective way to separate TAMs from colorectal carcinoma (CRC). Furthermore, we studied the best adherence time of separating macrophage among mononuclear cells. Methods specimens were collected from CRC patients , after digesting into single cells , TAMs were separated from the same specimen by 100% Ficoll, 35% percoll and 25% combined with 65% percoll respectively. After these pre-liminary separation, the collected cells were purified a second time by adherence separation. The purity of TAMs were detected by immunofluorescence. Results TAMs purity from Ficoll-Hypaque density gradient centrifugation was 80.18%, statistically higher than that from Percoll density gradient centrifugations (54.33% and 10.93% re-spectively). Conclusion Compared to Percoll density gradient centrifugation, Ficoll-Hypaque density gradient centrifugation is a more effective and simple way to isolate TAMs from colorectal carcinoma , suggesting it can be wildly used in clinical and basic medical research. 2-4 hours is the best adherence time for isolating macrophage.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of MALAT 1 in nasopharyngeal carcinoma (NPC) and its biological function.</p><p><b>METHOD</b>Real-time PCR was used to detect the expression of MALAT1 in 5-8F, C666-1, CNE-1, CNE-2, HONE-1, 6-10B and NP69 cell lines. CNE-1 cells engineered with MALAT1 RNA interference (RNAi) and RNA activation (RNAa) techniques were examined for changes in cell proliferation, invasion and metastasis using CCK8 assay, colony formation assay, Transwell in vitro invasion assay and wound-healing assay ability.</p><p><b>RESULTS</b>MALAT1 was highly expressed in 5-8F cells with a high metastatic potential, and lowly expressed in normal nasopharyngeal epithelium cells. Overexpression of MALAT1 by RNAa suppressed the expression of E-cadherin, promoted the expression of vimentin and enhanced the proliferation, invasion, and metastasis of CNE-1 cells.</p><p><b>CONCLUSION</b>MALAT1 can enhance the proliferation, invasion, and metastasis of CNE-1 cells.</p>
