ABSTRACT
In this study, we sequenced the complete mitochondrial DNA genome (mitogenome) of the Zhengyang Yellow chicken (Gallus gallus domesticus) by next-generation sequencing technology. Samples were taken from Zhumadian city, Henan Province, China. The complete mitogenome was 16 785 bp in size, and had a nucleotide composition of 30.3% (A), 23.7% (T), 32.5% (C), and 13.5% (G), with a high AT content of 54.0%. The assembled mitogenome exhibited typical mitochondrial DNA (mtDNA) structure, including a non-coding control region, two rRNA genes, 13 protein-coding genes, and 22 tRNA genes. Phylogenetic analysis indicated that this mitogenome defined a novel sub-haplogroup B3 within haplogroup B. These results should provide essential information for chicken domestication and insight into the evolution of genomes.
Subject(s)
Chickens/genetics , DNA, Mitochondrial/genetics , Genome, Mitochondrial/genetics , Animals , PhylogenyABSTRACT
Wuhua three-yellow chicken is a native breed of Guangdong Province in China. The complete mitochondrial DNA (mtDNA) genome presented here was the first assemble of Wuhua three-yellow chicken, which was determined through the polymerase chain reaction-based method. The complete mitogenome was 16,784 bp in length, with the nucleotide composition of 30.29% for A, 23.75% for T, 32.48% for C and 13.48% for G, and exhibited the typical mitochondrial structure, including 2 rRNA genes, 13 protein-coding genes, 22 tRNA genes and a non-coding control region.
