LML1, Encoding a Conserved Eukaryotic Release Factor 1 Protein, Regulates Cell Death and Pathogen Resistance by Forming a Conserved Complex with SPL33 in Rice.

Lesion mimic mutants are powerful tools for unveiling the molecular connections between cell death and pathogen resistance. Various proteins responsible for lesion mimics have been identified; however, the mechanisms underlying lesion formation and pathogen resistance are still unknown. Here, we identify a lesion mimic mutant in rice, lesion mimic leaf 1 (lml1). The lml1 mutant exhibited abnormal cell death and resistance to both bacterial blight and rice blast. LML1 is expressed in all types of leaf cells, and encodes a novel eukaryotic release factor 1 (eRF1) protein located in the endoplasmic reticulum. Protein sequences of LML1 orthologs are conserved in yeast, animals and plants. LML1 can partially rescue the growth delay phenotype of the LML1 yeast ortholog mutant, dom34. Both lml1 and mutants of AtLML1 (the LML1 Arabidopsis ortholog) exhibited a growth delay phenotype like dom34. This indicates that LML1 and its orthologs are functionally conserved. LML1 forms a functional complex with a eukaryotic elongation factor 1A (eEF1A)-like protein, SPL33/LMM5.1, whose mutant phenotype was similar to the lml1 phenotype. This complex was conserved between rice and yeast. Our work provides new insight into understanding the mechanism of cell death and pathogen resistance, and also lays a good foundation for studying the fundamental molecular function of Pelota/DOM34 and its orthologs in plants.

Effects of vanadate supply on plant growth, Cu accumulation, and antioxidant capacities in Triticum aestivum L.

The effects of normal vanadate (V) supply (40 µM) on copper (Cu) accumulation, plant growth and reduction in Cu toxicity in wheat seedlings (Triticum aestivum L.) were investigated. The results showed Cu accumulation (mg g(-1 )dw) in the applied V treatment was about 10.2 % in shoots and 16.7 % in roots higher up on exposure to excess Cu (300 µM) than that observed only in excess Cu plants. Compared with the treatment of the normal concentration used in Hoagland's culture solution Cu (0.6 µM), excess Cu significantly induced lipid peroxidation indicated by accumulation of thiobarbituric acid reactive substances (MDA). The seedlings showed apparent symptoms of Cu toxicity and plant growth were significantly inhibited by excess Cu. The applied V significantly decreased lipid peroxidation in roots caused by excess Cu and inhibited the appearance of Cu toxicity symptoms. Moreover, the applied V effectively improved the antioxidant defense system to alleviate the oxidative damage induced by Cu. Although the addition of V could promote superoxide dismutase in both shoots and roots to reduce superoxide radicals, peroxidase and catalase in shoots and ascorbate peroxidase and dehydroascorbate reductase in roots were major enzymes to eliminate H2O2 in wheat seedlings.

Characterization of Cu-tolerant bacteria and definition of their role in promotion of growth, Cu accumulation and reduction of Cu toxicity in Triticum aestivum L.

The effects of Cu-tolerant bacteria strain USTB-O on Cu accumulation, plant growth and reduction of Cu toxicity in wheat seedlings Triticum aestivum L. were investigated. The strain was identified as belonging to Bacillus species and showed a specific tolerance to Cu through binding the Cu ions to the cell walls to reduce their entry into the cells. The bacteria not only increased Cu accumulation in wheat seedlings, but also secreted indole-3-acetic acid (IAA) and therefore promoted plant growth. Moreover, the bacteria effectively improved the antioxidant defence system to alleviate the oxidative damage induced by Cu. The bacteria promoted superoxide dismutase (SOD) in both shoots and roots to reduce superoxide radicals. The bacteria stimulated all enzymes activities under Cu exposure conditions, peroxidase (POD) and catalase (CAT) in shoots and ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR) in roots were major enzymes to eliminate H2O2 in wheat seedlings.

Growth Control of Cyanobacteria by Three Submerged Macrophytes.

To illustrate the control of harmful cyanobacterial growth and the removal of nutritients from fresh water, three submerged macrophytes were grown in the raw water of Guishui Lake. Lindernia rotundifolia, Hygrophila stricta, and Cryptocoryne crispatula were grown together in situ to assess their effectiveness in nutrient removal in microcosms. Results revealed the inhibitory effects of these species on cyanobacterial growth. In addition, water quality in the planted microcosms showed improvement when compared to the water quality of the unplanted microcosm. At all treatments studied, the chemical oxygen demand in the planted microcosms was lower than that in the unplanted microcosms, and the removal rate of all the nitrogen and phosphate in the planted microcosms was better than that of the microcosm without plants. Our study offers a useful algal control method for the lakes or reservoirs that suffer from harmful cyanobacterial blooms.

Effect of organic ligands on accumulation of copper in hyperaccumulator and nonaccumulator Commelina communis.

Better understanding of copper uptake and accumulation regulation in plants is critical to the phytoremediation of copper contaminated soil. This study employed a 30-day pot experiment to assess the relationship between organic ligands and copper accumulation in plants. Hyperaccumulator and nonaccumulator varieties of Commelina communis were used, different organic ligands were applied, and the data of copper accumulation in shoots were collected. The six organic ligands included ethylenediaminetetraacetic acid and organic acids (formic acid, citric acid, malic acid, tartaric acid, and succinic acid). The results showed that organic ligands added to culture increased the copper accumulation both varieties. The results of the copper accumulation in shoots agreed with the study of the root uptake kinetics of copper influx. The addition of organic acids could increase copper accumulation in shoots because the copper influx in roots was increased. The results also indicated that the copper influx of hyperaccumulator roots was higher than that of nonaccumulator roots. This is one of the mechanisms by which a hyperaccumulator could amass large amounts of copper in its shoots. In this accumulation process, little effect on the leaf relative water content was in the hyperaccumulator and nonaccumulator of leaves and normal physiological condition of plants.