The application of varying amount of green manure combined with nitrogen fertilizer altered the soil bacterial community and rice yield in karst paddy areas.

Long-term application of green manure (GM) and nitrogen (N) fertilizers markedly improved soil fertility and boosted rice yield in ecologically fragile karst paddy fields. However, the precise response mechanisms of the soil bacterial community to varying amounts of green manure alone and in combination with N fertilizer in such environments remain poorly elucidated. In this study, we investigated the soil bacterial communities, keystone taxa, and their relationship with soil environmental variables across eight fertilization treatments. These treatments included group without N addition (N0M0, no N fertilizer and no GM; N0M22.5, 22.5 t/ha GM; N0M45, 45 t/ha GM, N0M67.5, 67.5 t/ha GM) and group with N addition (NM0, N fertilizer and no GM; NM22.5, N fertilizer and 22.5 t/ha GM; NM45, N fertilizer and 45 t/ha GM; NM67.5, N fertilizer and 67.5 t/ha GM). The results revealed that increasing green manure input significantly boosted rice yield by 15.51-22.08% and 21.84-35% in both the group without and with N addition, respectively, compared to N0M0 treatment. Moreover, with escalating green manure input, soil TN, AN, AK, and AP showed an increasing trend in the group without N addition. However, following the addition of N fertilizer, TN and AN content initially rose, followed by a decline due to the enhanced nutrient availability for rice. Furthermore, the application of a large amount of N fertilizer decreased the C: N ratio in the soil, resulting in significant changes in both the soil microbial community and its function. Particularly noteworthy was the transition of keystone taxa from their original roles as N-fixing and carbon-degrading groups (oligotrophs) to roles in carbon degradation (copiotrophs), nitrification, and denitrification. This shift in soil community and function might serve as a primary factor contributing to enhanced nutrient utilization efficiency in rice, thus significantly promoting rice yield.

Response of microbial communities and their metabolic functions to calcareous succession process.

Soil chronosequence is of great important in studying rates and directions of soil evolution, which can provide valuable information for the validation of soil genesis theory. However, the variation of microbial composition and structure in a calcareous soil chronosequence in karst region of southwest China is not clear. To reveal the response of microbial communities and their metabolic functions to calcareous succession process, a chronosequence of four calcareous soils (black calcareous soil, brown calcareous soil, yellow calcareous soil and red calcareous soil) with a depth of 0-20 cm from tropical monsoon rainforests of Guangxi Nonggang National Nature Reserve, southwest China was collected to analyze the soil physichemical and microbial properties. The results showed that the overall soil nutrient contents decreased along calcareous soil chronosequences and all calcareous soils were nitrogen (N) limitation. And, there were significant differences in the structure of microbial communities in calcareous soil chronosequences. To accommodate N-restriction, fungal community shifted from pathotroph to symbiotroph trophic pattern and Ectomycorrhizal fungi (ECM) emerged. ECM competing with free-living decomposers for N will slow soil carbon (C) cycling and increase soil C storage. Penicillium and Gaiella, the keystone genera, were related to phosphorus (P) cycle closely. Taken together, the occurrence of these microorganisms emphasizes the importance for C, N and P cycle in calcareous chronosequence soils and thus contributes to the ongoing worldwide endeavor to characterize their function for investigating the rate and direction of calcareous pedogenic changes.

Vegetation restoration facilitates belowground microbial network complexity and recalcitrant soil organic carbon storage in southwest China karst region.

Soil organic carbon (SOC) is an important component of soil ecosystems, and soils are a hotbed of microorganisms playing critical roles in soil functions and ecosystem services. Understanding the interaction between SOC and soil microbial community is of paramount significance in predicting the C fate in soils following vegetation restoration. In this study, high-throughput sequencing of 16S rRNA and ITS genes combined with 13C NMR spectroscopy analysis were applied to characterize SOC chemical compounds and elucidate associated soil microbial community. Our results indicated that the contents of SOC, total nitrogen, total phosphorus, microbial biomass carbon and biomass nitrogen, dissolved organic carbon, available potassium, exchangeable calcium and soil moisture increased significantly (P < 0.05) along with the vegetation restoration processes from corn land, grassland, shrub land, to secondary and primary forests. Moreover, the Alkyl C and O-alkyl C abundance increased with vegetation recovery, but no significant differences of Alkyl C were observed in different successional stages. In contrast, the relative abundance of Methoxyl C showed an opposite trend. The dominate phyla Proteobacteria, Acidobacteria, Actinobacteria, Ascomycota and Basidiomycota were strongly related to SOC. And, SOC was found to be the determining factor shaping soil bacterial and fungal communities in vegetation restoration processes. The complexity of soil bacteria and fungi interactions along the vegetation restoration chronosequence increased. Determinism was the major assembly mechanism of bacterial community while stochasticity dominated the assembly of fungal community. Bryobacter, Haliangium, and MND1 were identified as keystone genera in co-occurrence network. Besides, the dominant functional groups across all vegetation restoration processes were mainly involved in soil C and N cycles and linked to the enhanced recalcitrant SOC storage. Our results provide invaluable reference to advance the understanding of microbe response to vegetation restoration processes and highlight the impact of microbes on recalcitrant SOC storage.