ABSTRACT
The cell surface molecules controlling apoptosis in cortical neurons are largely unknown. A monoclonal antibody was derived that induces cultured neocortical neurons to undergo apoptosis. A Fab fragment of the antibody, however, lacked the ability to induce cell death. The antigen was purified, and characterized by compositional analysis, fast atom bombardment (FAB) mass spectrometry, sequential exoglycosidase treatments, methylation analysis, and (1)H-nuclear magnetic resonance spectroscopy, proving to be isoglobotetraosylceramide (IsoGb4). IsoGb4 has been shown previously to be a metastasis marker, antibodies against which block metastases in a mammary adenocarcinoma model (S. A. Carlsen et al., Cancer Res., 53: 2906-2911, 1993). Addition of the purified antigen to cells lacking this glycolipid demonstrated that it is capable of functioning as a portable apoptosis-transducing molecule. Intracellular ceramide levels were increased after the treatment with the apoptosis-inducing antibody, but the membrane sphingomyelin level remained unchanged. Fumonisin B1 inhibited both the ceramide increase and the apoptosis induced via IsoGb4, which indicated that the ceramide synthase pathway is likely to be involved in apoptosis induction by IsoGb4.
Subject(s)
Antibodies, Monoclonal/metabolism , Antigens, Surface/metabolism , Apoptosis/physiology , Globosides/metabolism , Neurons/cytology , Animals , Antibodies, Monoclonal/immunology , Antibodies, Monoclonal/pharmacology , Antigens, Surface/immunology , Antigens, Surface/isolation & purification , Apoptosis/immunology , Carbohydrate Sequence , Cell Transformation, Neoplastic , Globosides/immunology , Globosides/isolation & purification , HeLa Cells , Humans , Mice , Mice, Inbred BALB C , Molecular Sequence Data , Neurons/immunology , Neurons/metabolism , Signal Transduction/physiologyABSTRACT
Three of the predominant features of apoptosis are internucleosomal DNA fragmentation, plasma membrane bleb formation, and retraction of cell processes. We demonstrate that actin is a substrate for the proapoptotic cysteine protease interleukin 1beta-converting enzyme. Actin cleaved by interleukin 1beta-converting enzyme can neither inhibit DNase I nor polymerize to its filamentous form as effectively as intact actin. These findings suggest a mechanism for the coordination of the proteolytic, endonucleolytic, and morphogenetic aspects of apoptosis.
Subject(s)
Actins/metabolism , Apoptosis , Cysteine Endopeptidases/metabolism , Actin Cytoskeleton/metabolism , Actins/antagonists & inhibitors , Animals , Caspase 1 , Humans , Interleukin-1/metabolism , PC12 Cells , Peptide Mapping , Protein Binding , Rats , Substrate SpecificityABSTRACT
Glutamate has been shown, at high concentrations (5-10 mM), to lead to death in cells of the pheochromocytoma cell line PC12. We report that similar concentrations of glutamate also kill immortalized central neural cell lines, and that the expression of the proto-oncogene bcl-2 in these cell lines blocks glutamate neurotoxicity. Potassium chloride (25 mM) also protects a cerebellar neuronal cell line, but not PC12 cells, from glutamate toxicity.
Subject(s)
Cell Survival/drug effects , Glutamates/toxicity , Neurons/drug effects , Neurotoxins/toxicity , Protein-Tyrosine Kinases/metabolism , Proto-Oncogene Proteins/metabolism , Proto-Oncogenes , Transfection , Animals , Brain Neoplasms , CHO Cells , Cell Line, Transformed , Cerebellum , Cricetinae , Excitatory Amino Acid Antagonists , Glutamic Acid , Humans , Mice , Neurons/cytology , Neurotoxins/antagonists & inhibitors , PC12 Cells , Potassium Chloride/pharmacology , Proto-Oncogene Mas , Proto-Oncogene Proteins/biosynthesis , Proto-Oncogene Proteins c-bcl-2 , RatsABSTRACT
Nerve growth factor (NGF) binding to cellular receptors is required for the survival of some neural cells. In contrast to TrkA, the high-affinity NGF receptor that transduces NGF signals for survival and differentiation, the function of the low-affinity NGF receptor, p75NGFR, remains uncertain. Expression of p75NGFR induced neural cell death constitutively when p75NGFR was unbound; binding by NGF or monoclonal antibody, however, inhibited cell death induced by p75NGFR. Thus, expression of p75NGFR may explain the dependence of some neural cells on NGF for survival. These findings also suggest that p75NGFR has some functional similarities to other members of a superfamily of receptors that include tumor necrosis factor receptors, Fas (Apo-1), and CD40.
Subject(s)
Apoptosis , Nerve Growth Factors/metabolism , Neurons/cytology , Receptors, Nerve Growth Factor/physiology , Animals , Apoptosis/drug effects , Cell Line , Cell Survival/drug effects , Culture Media, Serum-Free , Nerve Growth Factors/pharmacology , Neurons/drug effects , Neurons/metabolism , PC12 Cells , Receptors, Nerve Growth Factor/metabolism , TransfectionABSTRACT
The protooncogene bcl-2, which has been implicated in B-cell lymphoma development, inhibits apoptosis due to growth factor withdrawal in some, but not all, hematopoietic cells. Recently we found that bcl-2 also inhibits apoptosis in PC12 pheochromocytoma cells. We now report that bcl-2 inhibits the death of a central neural cell line due to serum and growth factor withdrawal, the calcium ionophore A23187, glucose withdrawal, membrane peroxidation, and, in some cases, free radical-induced damage. This broad range of protective effects of BCL-2 protein suggests that BCL-2 may interact with a central step in neural cell death. Measurements of intracellular free calcium suggest that BCL-2 alters the transduction of neural death signals at a point distal to the rise in intracellular free calcium.
Subject(s)
Apoptosis , Cell Death , Neurons/cytology , Proto-Oncogene Proteins/physiology , Animals , Calcium/physiology , Gene Expression , In Vitro Techniques , PC12 Cells , Proto-Oncogene Proteins c-bcl-2 , Recombinant Proteins/metabolism , TransfectionABSTRACT
During development, many neuronal populations undergo a process of normal, programmed cell death, or apoptosis. Trophic factors regulate this process, but the mechanism by which they suppress apoptosis remains unclear. In the immune system, recent studies have implicated the protooncogene bcl-2 in the lymphocyte survival response to growth factors. To determine whether a similar survival pathway exists in a neuroendocrine cell type, we have expressed bcl-2 in the rat pheochromocytoma PC12 cell line and found that it abrogates the requirement for stimulation by growth factors to survive. bcl-2 expression also substantially delays the onset of injury by the calcium ionophore A23187.
