ABSTRACT
Burns and traumas are common injuries during both peace time and wartime. Lung is the earliest organ subjected to dysfunction and the incidence is highest. The systemic protective technology for the burn and trauma related lung injuries is based on evidence-based medicine and translational medicine. It includes a series of effective measures, such as rescue and treatment scheme for massive burn casualties, prophylactic tracheostomy, protective ventilation strategy, sequential cell protection, and prevention and treatment of sequelae, which prevents aggravation of lung injuries caused by ischemia reperfusion, oxidative stress, and iatrogenic factors, as well as reduces the incidence of complications to ensure the recovery after burns and traumas.
Subject(s)
Humans , Burns , Evidence-Based Medicine , Lung Injury , Translational Research, BiomedicalABSTRACT
<p><b>OBJECTIVE</b>To study the changes of neutral alpha-glucoside activity in the epididymis of heroin-dependent and heroin-withdrawal rats, and to investigate the effects of intervention with purine nucleotide (AMP and GMP).</p><p><b>METHODS</b>Eighty Wistar rats were randomly divided into 8 groups of equal number, control, nucleotide, heroin, heroin + nucleotide, 3 d withdrawal, 9 d withdrawal, 3 d nucleotide (nucleotide administrated for 3 days after heroin withdrawal) and 9 d nucleotide (nucleotide administrated for 9 days after heroin withdrawal). Neutral alpha-glucosidase activity in the epididymis was detected in each group of rats.</p><p><b>RESULTS</b>Compared with the control group, neutral alpha-glucoside activity was markedly decreased in the heroin group (P < 0.05), and also in the 3 d and 9 d withdrawal groups, although with no significant differences (P > 0.05).</p><p><b>CONCLUSION</b>Heroin reduces neutral alpha-glucoside activity in the epididymis of rats, and this effect may continue for some time after drug withdrawal, while purine nucleotide can keep neutral alpha-glucosidase activity in a relatively stable state.</p>
Subject(s)
Animals , Male , Rats , Epididymis , Chemistry , Heroin , Heroin Dependence , Metabolism , Purine Nucleotides , Pharmacology , Rats, Wistar , alpha-Glucosidases , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To analyze epidemiological characters of an outbreak of rotavirus diarrhea in Daxing County, Guangxi Province.</p><p><b>METHODS</b>Rotavirus-positive specimens were identified by ELISA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR. Full-length VP7 genes of 4 positive specimens were amplified and analyzed.</p><p><b>RESULTS</b>30 cases of Rotavirus-positive were identified from 64 specimens. The attack rate was 46.9%, and G/P typing was G1P[8]. A change of VP7 amino acid residue is at positions 68.</p><p><b>CONCLUSION</b>G1P[8] rotavirus is the etiologic agents of this diarrhea outbreak. In addition, adults were included in this outbreak.</p>
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Young Adult , Antigens, Viral , Genetics , Capsid Proteins , Genetics , China , Epidemiology , Diarrhea , Epidemiology , Virology , Disease Outbreaks , Feces , Virology , Genotype , Phylogeny , Rotavirus , Classification , Genetics , Rotavirus Infections , Epidemiology , VirologyABSTRACT
Two Rotavirus G9P[8] strains (LL52696 and LL52727) were recognized during a sentinel-based survey in Lulong, China. Phylogenetic analysis of the VP7 gene showed that both strains isolated constituted a divergent genetic cluster distinct from the other G9 strains isolated in China. Analysis of VP4, VP6, and NSP4 genes revealed that these strains were closely related to Lulong strains. We hold that two strains were reassortant between G9 and Lulong predominant strains.
Subject(s)
Humans , Amino Acid Sequence , Antigens, Viral , Chemistry , Genetics , Base Sequence , Capsid Proteins , Chemistry , Genetics , Glycoproteins , Chemistry , Genetics , Phylogeny , Rotavirus , Classification , Genetics , Toxins, Biological , Chemistry , Genetics , Viral Nonstructural Proteins , Chemistry , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To investigate if there is abnormal expression of androgen receptor (AR) in adult diabetic rats.</p><p><b>METHODS</b>The diabetic model rats were induced by using streptozotocin (STZ), which were divided into three groups: control (group C), diabetes (group D) and diabetes with insulin replacement group (group ID). The mRNA and protein expressions of androgen receptor in testis, epididymis and prostate were detected by Northern blot and radioimmunoassay, respectively.</p><p><b>RESULTS</b>Serum testosterone levels and AR mRNA in epididymis of group D or Group ID were lower than those of group C (P <0.05) , respectively, and the protein expression of AR in testis and prostate of group D was lower than that of group C (P <0.05).</p><p><b>CONCLUSION</b>The expression of androgen receptor decreased in testis, epididymis and prostate of diabetic rats, which weakened the biological effects of AR, and it might be one of the causes that resulted in the sexual and productive dysfunction in diabetic rats.</p>
Subject(s)
Animals , Male , Rats , Diabetes Mellitus, Experimental , Metabolism , Epididymis , Metabolism , Prostate , Metabolism , RNA, Messenger , Genetics , Rats, Wistar , Receptors, Androgen , Genetics , Testis , MetabolismABSTRACT
<p><b>OBJECTIVES</b>To study the effect of antisperm antibody(AsAb) on human sperm acrosin activity.</p><p><b>METHODS</b>AsAb and sperm acrosin activity were measured and analyzed in 3,432 infertile men and 65 fertile volunteers.</p><p><b>RESULTS</b>AsAb positive rate was 10.20% in 3,432 case of male infertility, and 9.37% in 2,882 infertile males who received tests of sperm acrosin activity. Acrosin activity of infertility cases were lower than those of fertile cases(P < 0.001). The comparison between AsAb positive group and AsAb negative group infertility cases showed no significant differences of acrosin activity (P > 0.05). Between normal acrosin activity group and abnormal acrosin activity group, there was no significant difference of AsAb positive rate (P > 0.05).</p><p><b>CONCLUSIONS</b>Antisperm antibody could not affect acrosin activity.</p>
Subject(s)
Adult , Humans , Male , Acrosin , Metabolism , Autoantibodies , Case-Control Studies , Infertility, Male , Allergy and Immunology , Semen , Chemistry , Spermatozoa , Allergy and ImmunologyABSTRACT
<p><b>OBJECTIVES</b>To study the changes of sexual gland 5 alpha-reductase type II activity in pubertal and adult rats with diabetes.</p><p><b>METHODS</b>We selected 40 and 90 days old male Wistar rats as pubertal and adult animal model respectively, 30 rats in each group. The rats were randomly divided into three groups: control group (C), diabetic group (D) and diabetes with insulin replacement group (ID). The activity of 5 alpha-reductase type II was measured with thin layer chromatography in the epididymis, prostate and testis.</p><p><b>RESULTS</b>1. In all sexual glands of pubertal rats, the activity of 5 alpha-reductase type II in D group is significantly lower than that in C and ID groups. 2. In all sexual glands of adult rats. there is no difference in the activity of 5 alpha-reductase type II among these groups.</p><p><b>CONCLUSIONS</b>The activity of 5 alpha-reductase type II is likely to be influenced by metabolic environment, hormonal levels and local specific factors in pubertal rats, but it is relatively stable in adult rats.</p>
