ABSTRACT
Objective:To explore the role and mechanism of 4-1BBL and B7-1 molecules in the activation and proliferation of human peripheral blood T lymphocyte. Methods:T lymphocyte was purified by magnetic beads negative selecting. The proliferation of T cells in primary allogenic MLR was evaluated by using 3H-TdR incorporation; The immunophenotype of T cells was analyzed by FACS and the quantitative measurement of IL-2 in culture supernatant were performed by ELISA.Results:After negative selection, the purity of CD3+ T cells was over 90%; 4-1BBL and B7-1 molecules stably expressed on XG cells after 4-1BBL and B7-1 cDNA transfection; 4-1BBL or B7-1 gene transfected XG cells could more effectively mediate the activation, proliferation and IL-2 secretion of T cells than XG cells did, and the results also showed that the costimulatory molecules had a co-activating effect on T cells.Conclusion:Human multiple myeloma cells failed to induced antitumor immunoresponse because of the lack or weak expression of costimulatory molecules. The costimulatory molecules of 4-1BBL and B7-1 has a synthetic effect on endowing myeloma cells with enhancing the activation、proliferation and IL-2 secretion of T cells in vitro. [
