ABSTRACT
Objective:To analyze the relationship between the AXIN2 gene promoter methylation status in breast cancer tissues with the breast cancer-related risk factors, clinicopathological characteristics and the mRNA expression of AXIN2 gene.Methods:The breast cancer tissues and adjacent tissues of 84 breast cancer patients were taken to detect the AXIN2 gene promoter methylation status and the AXIN2 mRNA expression. We used different concentrations of methyltransferase inhibitors 5-Azacytidine (5 -Aza) to treat human breast cancer ZR-75-1 cells, and collected the breast cancer-related risk factors and clinicopathological characteristics.Results:The positive rate of methylation of AXIN2 gene promoter in breast cancer tissues (40.5%) was significantly higher than that of adjacent tissues (19.0%) ( χ2=10.401, P=0.001) . The positive rate of AXIN2 methylation was related to the age at first delivery ( χ2=5.467, P=0.019) and a history of induced abortion ( χ2=8.372, P=0.006) ; the positive rate of AXIN2 methylation was related to lymph node metastasis ( χ2=5.338, P=0.021) , ER expression status ( χ2=9.141, P=0.002) and PR expression status ( χ2=6.918, P=0.009) . In addition, the relative expression of AXIN2 gene mRNA in breast cancer tissues (0.22 ± 0.03) was significantly lower than that in adjacent tissues (0.46 ± 0.06) ( t=3.527, P<0.001) . The relative mRNA expression of the methylation-positive breast cancer tissues (0.13 ± 0.02) was significantly lower than that of the methylation-negative breast cancer tissues (0.29 ± 0.04) ( t=3.616, P<0.001) . After ZR-75-1 cells treated with 5-Aza, the relative expression of AXIN2 gene mRNA increased significantly ( t=3.824, P<0.001) . Conclusion:DNA methylation in the promoter region of the AXIN2 gene is related to the pathogenesis of breast cancer, and has certain clinical application value.
ABSTRACT
Adjuvant chemotherapy does not achieve the desired therapeutic efficacy in colon cancer as a result of the deficient reaction. Gene therapy using small interfering RNAs (siRNAs) delivered by target delivering system represents a potent and specific strategy in tumor therapy. Integrinß6 is exclusively expressed in malignant colonic epithelia, associated with the progression, metastasis, and chemotherapeutic resistance of colon cancer. Accordingly, designing an efficient and targeted delivery system for ß6-siRNA could be a potential approach to improve therapeutic efficacy of colon cancer. Here, we designed the Integrinß6 target immunoliposomes for highly efficient and selective delivery of ß6-siRNA in colon cancer, which consequently resulted in greatly growth suppression, invasion and metastasis of colon cancer cells. Moreover, it was able to greatly inhibit the tumor growing in vivo.
Subject(s)
Colon/pathology , Colonic Neoplasms/therapy , Integrin beta Chains/genetics , Intestinal Mucosa/physiology , RNA, Small Interfering/genetics , Animals , Cell Growth Processes , Cell Line, Tumor , Cell Movement , Colonic Neoplasms/genetics , Drug Delivery Systems , Female , Gene Expression Regulation, Neoplastic , Humans , Liposomes/chemical synthesis , Mice , Mice, Inbred BALB C , Mice, Nude , Neoplasm Metastasis , RNA, Small Interfering/chemical synthesis , Tumor Burden , Xenograft Model Antitumor AssaysABSTRACT
Objective To analyze the value of carbon nanoparticles in the cleaning of thyroid cancer lymph node.Methods 80 patients with thyroid cancer from March 2012 to March 2013 were included in this study.Based on the sequence of enrollment,they were randomly divided into two groups:the therapy group (n=40)were received Nano carbon lymphatic tracer before basic operative treatment and the control group (n=40)were directly received the same basic operative treatment.The liver function parameters in two groups were observed and compared statistically. Results The operating time,operative blood loss and postoperative drainage volume had no statistically significant difference between two groups.The incidence of postoperative hypocalcemia in experimental group was lower than that in blank control group(P<0.05 ).The parathyroid hormone level in experimental group on the first postoperative day was higher than that in blank control group(P<0.05 ).The number of resected lymph nodes in experimental group was more than that in blank control group(P<0.01).Conclusion Nano carbon lymphatic tracer technique can increase the cleaning number of lymph node of thyroid cancer patients in central region,and realize the accurating tumor stage.
