ABSTRACT
The method is based on the enhancement effect of the resonance light-scattering (RLS) of pararosaniline by DNA. In the pH rang of 0.5-1.5, the enhanced intensity of pararosaniline RLS at 355 nm is proportional to the concentration of DNA in the rang of 0.20-15 micrograms.mL-1. The detection limit is 36 ng.mL-1. This method is simple, rapid and has been applied satisfactorily to the determination of DNA in mixed samples.
Subject(s)
DNA/analysis , Rosaniline Dyes , Toluidines , Animals , Humans , Hydrogen-Ion Concentration , Scattering, Radiation , Sensitivity and Specificity , Spectrometry, Fluorescence , Spectrophotometry/methodsABSTRACT
A new selective and sensitive spectrofluorimetric method for the determination of Tyrosine in beer and grape wine has been developed. This method is based on the quenching of fluorescence of Tyrosine by Molybrum(VI). The exitation and emission wavelengths were found to be 278 and 305 nm, respectively. There was a linear relationship in the range of 0.01-14.4 micrograms.mL-1 for Tyrosine. This method is simple and rapid, and has been applied to the determination of Tyrosine in beer and grape wine with satisfactory results.
