ABSTRACT
We conducted an in situ incubation experiment to determine soil mineral N (NH4+-N and NO3--N) concentrations and soil net N mineralization rates (net ammonification rate and net nitrification rate) using close-top PVC tubes in three adjacent forests (natural forest, Castanopsis kawakamii and Cunninghamia lanceolata plantation) from September 2014 to August 2015 in subtropical China, investigating the effects of forest type and season on soil inorganic N concentrations and soil net N mineralization rates. Results showed that soil NO3--N was the dominant form in mi-neral N pool in all three forests, and the proportion of NO3--N to soil inorganic N content ranged from 55.1% to 87.5% and from 56.1% to 79.1% in natural forest and Cunninghamia lanceolata plantation, respectively. The effects of forest types on soil mineral N pool were only significant on soil NO3--N, and the concentration of NO3--N in Castanopsis kawakamii was significantly lower than in the other two forests. The NO3--N and mineral N pool varied seasonally in all forests, and were higher in dormant season (October to February) than in growing season (March to September). Soil nitrification rate was very low in the whole year in all three forests and soil net ammonification was the major process of soil net mineralization. Tree species significantly affected soil net ammonification rate, and the value under Chinese fir was significantly lower than the other two fore-sts. The seasonal patterns of the soil net ammonification rate were not similar in all the three forests, but with the lowest value occurring in November and February in the following year. Analysis using variance of repeated measures indicated that soil mineral N concentrations and soil N mineralization rates were significantly affected by forest type and season, and correlation analysis showed that soil mineral N and soil N mineralization rate were significantly affected by water moisture and temperature, and the effects of litter on soil N mineralization rate were mainly through quality control, ra-ther than the quantity control.
Subject(s)
Forests , Nitrogen , China , Minerals , Seasons , Soil , TreesABSTRACT
Forest types have significant effects on the availability and dynamics of soil dissolved organic carbon (DOC) and dissolved organic nitrogen (DON). By now the impacts of forest types on soil DOC and DON were mainly focused on surface soil (0-10 cm). Based on the comparisons between natural forest, Phyllostachys pubescens, Castanopsis kawakamii and Cunninghamia lanceolata plantations, we investigated the effects of forest types on soil DOC and DON pools in top (0-10 cm) and deep soils (40-60 cm). Cold water, hot water and KCl solutions were used to extract soil DOC and DON from surface and deep soils. Results showed that the effects of forest types on soil DOC, DOC/TOC, DON and soil microbial biomass carbon were only significant in the surface soil. The concentrations of DOC and DON varied with extract methods and hot water extracted the largest amounts of DOC and DON, and cold water the least. Correlations among hot water, KCl and cold water extracted DOC and DON were significant, suggesting that the organic C and N released by these three solutions might be at least partly from similar pools. The concentrations of DOC and DON and DOC/TOC in surface soil under natural forest and P. pubescens were greater than under C. kawakamii and C. lanceolata. It indicated that the concentrations of DOC and DON were greater under the natural forest and P. pubescens than under the C. kawakamii and C. Lanceolata, and more beneficial to improve soil fertility.
Subject(s)
Carbon/analysis , Forests , Nitrogen/analysis , Soil/chemistry , Biomass , China , Cunninghamia , Poaceae , Soil MicrobiologyABSTRACT
Tuberculosis remains a global public health problem in recent years. To develop novel type of potential antitubercular agents, twelve novel dihydroartemisinin-fluoroquinolone (DHA-FQ) conjugates (three types of molecules) were gradually designed and conveniently synthesized. All the newly synthesized conjugates were well characterized and evaluated against different Mycobacterium tuberculosis strains in vitro. The screening results showed that five DHA-FQ conjugates were active toward M. tuberculosis H37Rv, and compound 3a exhibited the strongest inhibitory activity (MIC=0.0625 µg/mL), which was comparable to the positive control Moxifloxacin and even stronger than Ofloxacin. Conjugates 2a and 3a also displayed comparable activities against various clinically isolated sensitive and resistant M. tuberculosis strains (MIC=0.125-16 µg/mL) to Moxifloxacin. All target compounds possessed selective anti-M. tuberculosis ability. Preliminary structure-activity relationship demonstrated that short linker between DHA and FQ was favorable for strong antitubercular activity. This study provides a new clue for the development of novel antitubercular lead molecules.
