ABSTRACT
OBJECTIVE: To research the hereditary stability of Dendrobium huoshanense which were subcultured 7-8 times in the same tissue culture system. METHOD: Using three primers of arbitrary decamer oligonucleotide sequences from 20 primers for DNA amplification, random amplified polymorphic DNA (RAPD) were performed. RESULT: The genetic similarity coefficient was varied from 85.4% to 98.4%. The variation of protocorm, germination and monoleaf were rather more notable than that of bileaf and inflorescence. CONCLUSION: The variation of the same pod is quite small. It is feasible to set up the rapid propagation system of D. huoshanense
Subject(s)
DNA, Plant/genetics , Dendrobium/genetics , Genetic Variation , Plants, Medicinal/genetics , Analysis of Variance , DNA Primers , DNA, Plant/analysis , Dendrobium/growth & development , Germination/genetics , Plants, Medicinal/growth & development , Random Amplified Polymorphic DNA Technique , Time Factors , Tissue Culture TechniquesABSTRACT
The biogenesis of chloroplast from proplastid is the prerequisite of photosynthesis. Using electron microscope, we found that rice albino mutant Osalb23 had no thylakoid inside the chloroplast, only some empty vesicles could be observed (Fig. 2). Genetics analysis showed that albino phenotype was controlled by a single recessive locus. Using map-based cloning technique, OsALB23 has been mapped to a region of 280 kb between molecular markers R2M501 and R2M502 on chromosome 2 (Fig. 4). Homologous analysis indicated that this region contained six chloroplast protein genes.
Subject(s)
Chloroplasts/metabolism , Chromosome Mapping/methods , Oryza/genetics , Plant Proteins/genetics , Chromosomes, Plant/genetics , Mutation , Oryza/growth & development , Plant Proteins/physiology , Plants, Genetically Modified/genetics , Plants, Genetically Modified/growth & developmentABSTRACT
It has been more than a centaury since the identification of ethylene as a gaseous plant hormone, and the effects of ethylene on plants have enabled broad applications in agriculture. Efforts had been made to understand ethylene signal transduction in plants, but it had not been successful until the identification and cloning of the first plant hormone receptor gene, ETR1, in Arabidopsis a decade ago. Comprehensive genetic studies allow the dissecting of the signaling pathway to reveal the components involved, which makes the studies of ethylene signal transduction become one the most-understood signal transduction pathways in plants. The identification of the signaling components and the genetic model of ethylene signal transduction pathway are described in this review.
Subject(s)
Arabidopsis/physiology , Ethylenes/pharmacology , Plant Proteins/physiology , Receptors, Cell Surface/physiology , Signal Transduction/physiology , Arabidopsis/genetics , Models, Genetic , Plant Proteins/chemistry , Receptors, Cell Surface/chemistryABSTRACT
This paper summarizes the studies on improving crop nutritional quality including protein, saccharide and lipid through gene engineering in recent 10 years. Special emphasis is laid upon the improvement of protein contains and amino acid components. The food safety caused probably by gene engineering and some ways to solve the problem are introduced briefly.