ABSTRACT
Five new drimane sesquiterpenes (1-5) together with 14 known analogues (6-19) were isolated from laboratory cultures of a mangrove-derived fungus Aspergillus ustus. Their structures were established by spectroscopic methods and antitumor activities were evaluated by sulforhodamine B (SRB) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) methods.
Subject(s)
Antineoplastic Agents/chemistry , Aspergillus/chemistry , Sesquiterpenes/chemistry , Antineoplastic Agents/isolation & purification , Antineoplastic Agents/toxicity , Magnetic Resonance Spectroscopy , Molecular Conformation , Polycyclic Sesquiterpenes , Sesquiterpenes/isolation & purification , Sesquiterpenes/toxicityABSTRACT
Six new prenylated polyhydroxy-p-terphenyl metabolites, named prenylterphenyllins A-C (1-3) and prenylcandidusins A-C (5-7), and one new polyhydroxy-p-terphenyl with a simple tricyclic C-18 skeleton, named 4''-dehydro-3-hydroxyterphenyllin (4), were obtained together with eight known analogues (8-15) from Aspergillus taichungensis ZHN-7-07, a root soil fungus isolated from the mangrove plant Acrostichum aureum. Their structures were determined by spectroscopic methods, and their cytotoxicity was evaluated using HL-60, A-549, and P-388 cell lines. Compounds 1 and 8 exhibited moderate activities against all three cell lines (IC50 1.53-10.90 µM), whereas compounds 4 and 6 displayed moderate activities only against the P-388 cell line (IC50 of 2.70 and 1.57 µM, respectively).
