ABSTRACT
Objective: Genomic alterations and potential neoantigens for cervical cancer immunotherapy were identified in a cohort of Chinese patients with cervical squamous cell carcinoma (CSCC). Methods: Whole-exome sequencing was used to identify genomic alterations and potential neoantigens for CSCC immunotherapy. RNA Sequencing was performed to analyze neoantigen expression. Results: Systematic bioinformatics analysis showed that C>T/G>A transitions/transversions were dominant in CSCCs. Missense mutations were the most frequent types of somatic mutation in the coding sequence regions. Mutational signature analysis detected signature 2, signature 6, and signature 7 in CSCC samples. PIK3CA, FBXW7, and BICRA were identified as potential driver genes, with BICRA as a newly reported gene. Genomic variation profiling identified 4,960 potential neoantigens, of which 114 were listed in two neoantigen-related databases. Conclusion: The present findings contribute to our understanding of the genomic characteristics of CSCC and provide a foundation for the development of new biotechnology methods for individualized immunotherapy in CSCC.
Subject(s)
Carcinoma, Squamous Cell , Immunotherapy , Uterine Cervical Neoplasms , Humans , Female , Uterine Cervical Neoplasms/genetics , Uterine Cervical Neoplasms/therapy , Uterine Cervical Neoplasms/immunology , Carcinoma, Squamous Cell/genetics , Carcinoma, Squamous Cell/therapy , Carcinoma, Squamous Cell/immunology , Cohort Studies , Antigens, Neoplasm/genetics , Antigens, Neoplasm/immunology , Middle Aged , Exome Sequencing , China , Mutation , Adult , Genomics , East Asian PeopleABSTRACT
The near infrared (NIR) spectroscopy analytical technique is one of the most advanced and promising tools in many domains. NIR acquisition is easily influenced by temperature, thereby affecting qualitative and quantitative analyses. In this paper, a temperature compensation model was established between NIR signals and output voltage values based on two-dimensional regression analysis. The effectiveness of the proposed compensation scheme was experimentally demonstrated by the measurement of six super luminescent diode sources at 293-313 K. The coefficient of variation was decreased 2-fold with this compensation algorithm. The results indicated that it was suitable for various NIR spectral acquisition systems with lower complexity and a higher signal-noise-ratio after being applied to an acousto-optic-tunable-filter system.
