Significant Difference in Hydrogen Isotope Composition Between Xylem and Tissue Water in Populus Euphratica.

Deuterium depletions between stem water and source water have been observed in coastal halophyte plants and in multiple species under greenhouse conditions. However, the location(s) of the isotope fractionation is not clear yet and it is uncertain whether deuterium fractionation appears in other natural environments. In this study, through two extensive field campaigns utilizing a common dryland riparian tree species Populus euphratica Oliv., we showed that no significant δ(18) O differences were found between water source and various plant components, in accord with previous studies. We also found that no deuterium fractionation occurred during P. euphratica water uptake by comparing the deuterium composition (δD) of groundwater and xylem sap. However, remarkable δD differences (up to 26.4‰) between xylem sap and twig water, root water and core water provided direct evidence that deuterium fractionation occurred between xylem sap and root or stem tissue water. This study indicates that deuterium fractionation could be a common phenomenon in drylands, which has important implications in plant water source identification, palaeoclimate reconstruction based on wood cellulose and evapotranspiration partitioning using δD of stem water.

Molecular mechanisms of foliar water uptake in a desert tree.

Water deficits severely affect growth, particularly for the plants in arid and semiarid regions of the world. In addition to precipitation, other subsidiary water, such as dew, fog, clouds and small rain showers, may also be absorbed by leaves in a process known as foliar water uptake. With the severe scarcity of water in desert regions, this process is increasingly becoming a necessity. Studies have reported on physical and physiological processes of foliar water uptake. However, the molecular mechanisms remain less understood. As major channels for water regulation and transport, aquaporins (AQPs) are involved in this process. However, due to the regulatory complexity and functional diversity of AQPs, their molecular mechanism for foliar water uptake remains unclear. In this study, Tamarix ramosissima, a tree species widely distributed in desert regions, was investigated for gene expression patterns of AQPs and for sap flow velocity. Our results suggest that the foliar water uptake of T. ramosissima occurs in natural fields at night when the humidity is over a threshold of 85 %. The diurnal gene expression pattern of AQPs suggests that most AQP gene expressions display a circadian rhythm, and this could affect both photosynthesis and transpiration. At night, the PIP2-1 gene is also upregulated with increased relative air humidity. This gene expression pattern may allow desert plants to regulate foliar water uptake to adapt to extreme drought. This study suggests a molecular basis of foliar water uptake in desert plants.

Foliar water uptake of Tamarix ramosissima from an atmosphere of high humidity.

Many species have been found to be capable of foliar water uptake, but little research has focused on this in desert plants. Tamarix ramosissima was investigated to determine whether its leaves can directly absorb water from high humidity atmosphere and, if they can, to understand the magnitude and importance of foliar water uptake. Various techniques were adopted to demonstrate foliar water uptake under submergence or high atmospheric humidity. The mean increase in leaf water content after submergence was 29.38% and 20.93% for mature and tender leaves, respectively. In the chamber experiment, obvious reverse sap flow occurred when relative humidity (RH) was persistently above 90%. Reverse flow was recorded first in twigs, then in branches and stems. For the stem, the percentage of negative sap flow rate accounting for the maximum value of sap flow reached 10.71%, and its amount accounted for 7.54% of diurnal sap flow. Small rainfall can not only compensate water loss of plant by foliar uptake, but also suppress transpiration. Foliar uptake can appear in the daytime under certain rainfall events. High atmospheric humidity is beneficial for enhancing the water status of plants. Foliar uptake should be an important strategy of water acquisition for desert plants.

Origins of groundwater inferred from isotopic patterns of the Badain Jaran Desert, northwestern China.

There are many viewpoints about the sources of groundwater in the Badain Jaran Desert (BJD), such as precipitation and snowmelt from the Qilian Mountains (the upper reaches [UR] of the Heihe River Basin [HRB]) and precipitation from the BJD and the Yabulai Mountains. To understand the source of the groundwater of the BJD and their possible associations with nearby bodies of water, we analyzed variations of stable isotope ratios (δD and δ(18) O) and the deuterium excess (d-excess) of groundwater and precipitation in the BJD, of groundwater, precipitation, river and spring water in the UR, and of groundwater and river water in the middle and lower reaches (MR and LR) of the HRB. In addition, the climatic condition under which the groundwater was formed in the BJD was also discussed. We found obvious differences in δD, δ(18) O, and d-excess among groundwater in the BJD, nearby water bodies and the HRB. The groundwater δD-δ(18) O equation for the BJD was δD = 4.509δ(18) O-30.620, with a slope and intercept similar to that of nearby areas (4.856 and -29.574), indicating a strong evaporation effect in the BJD and its surrounding areas. The equation's slope of the BJD was significantly lower than those of HRB groundwater (6.634), HRB river water (6.202), precipitation in the BJD and Youqi (7.841), and the UR of the HRB (7.839). The d-excess (-17.5‰) of the BJD was significantly lower than those of nearby groundwater (-7.4‰), HRB groundwater (12.1‰), precipitation in the BJD (5.7‰) and in the UR of the HRB (15.2‰), and HRB river water (14.4‰). The spatial patterns of δ(18) O and d-excess values in the BJD suggest mixing and exchange of groundwater between the BJD and neighboring regions, but no hydraulic relationship between the BJD groundwater and water from more distant regions except Outer Mongolia, which is north of the BJD. Moreover, we conclude that there is little precipitation recharge to groundwater because of the obvious d-excess difference between groundwater and local precipitation, low precipitation, and high evaporation rates. The abnormally negative d-excess values in groundwater of the BJD indicate that this water was formed in the past under higher relative humidity and lower temperatures than modern values.

Detailed assessment of isotope ratio infrared spectroscopy and isotope ratio mass spectrometry for the stable isotope analysis of plant and soil waters.

As an alternative to isotope ratio mass spectrometry (IRMS), the isotope ratio infrared spectroscopy (IRIS) approach has the advantage of low cost, continuous measurement and the capacity for field-based application for the analysis of the stable isotopes of water. Recent studies have indicated that there are potential issues of organic contamination of the spectral signal in the IRIS method, resulting in incorrect results for leaf samples. To gain a more thorough understanding of the effects of sample type (e.g., leaf, root, stem and soil), sample species, sampling time and climatic condition (dry vs. wet) on water isotope estimates using IRIS, we collected soil samples and plant components from a number of major species at a fine temporal resolution (every 2 h for 24-48 h) across three locations with different climatic conditions in the Heihe River Basin, China. The hydrogen and oxygen isotopic compositions of the extracted water from these samples were measured using both an IRMS and an IRIS instrument. The results show that the mean discrepancies between the IRMS and IRIS approaches for δ(18) O and δD, respectively, were: -5.6‰ and -75.7‰ for leaf water; -4.0‰ and -23.3‰ for stem water; -3.4‰ and -28.2‰ for root water; -0.5‰ and -6.7‰ for xylem water; -0.06‰ and -0.3‰ for xylem flow; and -0.1‰ and 0.3‰ for soil water. The order of the discrepancy was: leaf > stem ≈ root > xylem > xylem flow ≈ soil. In general, species of the same functional types (e.g., woody vs. herbaceous) within similar habitats showed similar deviations. For different functional types, the differences were large. Sampling at nighttime did not remove the observed deviations.