The mating system of Himalayan marmots as inferred by microsatellite markers.

The Himalayan marmot Marmota himalayana is widely distributed across the Qinghai-Tibetan Plateau and lives in social groups, yet the mating system of this highly social marmot species is unknown. In this study, the genetic mating system of Himalayan marmots was investigated using microsatellite markers to determine which mating strategies individuals employ. Results revealed that both monogamous and polygamous mating relationships occur in our study population, indicating that the genetic mating system of this marmot species is promiscuity. This study presents the first genetic evidence on the mating system for Himalayan marmots, yet indicates that further studies employing both a genetic and behavioral framework are needed to better understand the social structure and reproductive biology of this marmot species.

Fatty acid metabolization and insulin regulation prevent liver injury from lipid accumulation in Himalayan marmots.

Fat storage and weight gain are dominant traits for hibernating mammals. However, excessive fat accumulation may cause liver damage. Here, we explore the lipid accumulation and metabolic processes of the Himalayan marmot (Marmota himalayana), a hibernating rodent species. We find that the unsaturated fatty acid (UFA) content in food was consistent with a large increase in the body mass of Himalayan marmots. Metagenomic analysis shows that Firmicutes Bacterium CAG:110 plays a synergistic role by synthesizing UFAs, which is demonstrated by fecal transplantation experiments, indicating that the gut microbiome promotes fat storage in Himalayan marmots for hibernation. Microscopic examination results indicate that the risk of fatty liver appears at maximum weight; however, liver function is not affected. Upregulations of UFA catabolism and insulin-like growth factor binding protein genes provide an entry point for avoiding liver injury.

Effects of human activity on the habitat utilization of Himalayan marmot (Marmota himalayana) in Zoige wetland.

Human activity is increasingly and persistently disturbing nature and wild animals. Affected wildlife adopts multiple strategies to deal with different human influences. To explore the effect of human activity on habitat utilization of Himalayan marmot (Marmota himalayana), habitat utilization patterns of three neighboring marmot populations in habitats affected differently by human activities were recorded and compared. We found that (a) distance between reproductive burrows (a represent of reproductive pairs) becomes shorter under the influence of human activities, and more burrows were dug as temporary shelters, resulting in shorter distance between those shelters and shorter distance flee to those shelters and, consequently, shorter flight initiation distance when threatened. More burrows that are closer to the disturbed habitats improve the ability to escape from threats. (b) Reproductive burrow site selection of the species is determined by the availability of mounds in the habitat, and breeding pairs selectively build reproductive (also the hibernation) burrows on mounds, potentially to improve surveillance when basking and the drainage of burrows. Human activities generally drive breeding pairs away from the road to dig their reproductive burrows likely to reduce disturbance from vehicles. However, even heavy human activity exerts no pressure on the distance of reproductive burrows from the road or the mound volume of the high disturbance population, potentially because mounds are the best burrowing site to reproduce and hibernate in the habitat. Marmots deal with disturbance by digging more burrows in the habitat to flee more effectively and building reproductive burrows on mounds to gain better vigilance and drainage efficiency.

Burrowing and Anti-Predator Requirements Determine the Microhabitat Selection of Himalayan Marmot in Zoige Wetland.

To satisfy their requirements for food and safety, animals need certain habitats to live. Marmots generally select habitats with certain elevation, land surface temperature, soil and vegetation type, and certain mountain slope and aspect; however, what habitats are needed at relatively smaller scales are poorly known. The Himalayan marmot (Marmota himalayana) is distributed mainly on the Qinghai-Tibet Plateau, a region exhibiting diversified topographic features, and the Zoige wetland in the northeast part of the plateau is also the home of the Himalayan marmot. The region is famous for its plateau peat bog, and the suitable habitats for Himalayan marmots are patchily distributed in the wetland. To investigate what kinds of patches are preferred by the marmot in this wetland ecosystem, we measured and compared the soil and vegetation characteristics of used and unused patches. We found that unlike factors governing the habitat selection at macroscales, patches characterized by flat ground and low soil moisture content, with medium vegetation standing height and low vegetation density, are selected in the Zoige wetland. Patches of this kind are selected to meet the marmots' requirements for burrow construction and predator avoidance in such a wetland ecosystem. Together with previous studies on habitat selection of the marmot species at macroscales, we showed that to explore how the animals survive in an environment, it is important to conduct the analysis at multiple scales.