Long-Chain Non-Coding RNA SNHG3 Promotes the Growth of Ovarian Cancer Cells by Targeting miR-339-5p/TRPC3 Axis.

BACKGROUND: Long-chain non-coding RNA (lncRNA) small nucleolar RNA host gene 3 (SNHG3) is reportedly overexpressed in malignant tumors, but its regulatory role in human ovarian cancer (OC) is not fully understood. METHODS: A qRT-PCR assay was carried out to detect the level of SNHG3 in OC tissues, serum and cells, a CCK-8 assay to measure the proliferation of OC cells, a transwell assay to measure the invasion and migration of OC cells, and a flow cytometry to detect the cell cycle distribution and apoptosis rate of OC cells. In addition, in vivo experiment was also conducted to determine the effect of SNHG3 on the growth of OC cells. RESULTS: SNHG3 was overexpressed in OC tissues, serum, and cells, and the overexpression in serum indicated a poor prognosis of patients. It was also found that knockdown of SNHG3 could inhibit the malignant phenotypes of OC cells, cause G1/G0 cell cycle arrest, and intensify apoptosis. Furthermore, in in vitro experiments, the growth ability of OC cells was inhibited under knockdown of SNHG3. Assays for relationship verification showed that SNHG3 regulated the expression of miR-339-5p and the canonical transient receptor potential 3 (TRPC3), and the rescue experiment revealed that co-transfection of si-SNHG3+miR-339-5p-inhibitor or si-SNHG3+pcDNA3.1-TRPC3 could reverse the effects of knockdown of SNHG3 on the biological behavior of OC cells. CONCLUSION: SNHG3 can be adopted as a marker for diagnosis and prognosis evaluation of OC and it plays a role in the progression of OC by enabling the miR-339-5p sponge to regulate TRPC3 expression.

Controlling plasmon-induced transparency of graphene metamolecules with external magnetic field.

We numerically demonstrate dynamically tuneable plasmon-induced transparency in a π-shaped metamolecules made of graphene nanostrips by applying external static magnetic field. It is shown that for graphene nanostrips with appropriate Fermi energy, the resonant wavelength, line-shape, and the polarization of transmitted light in the mid-infrared can be effectively controlled by magnetic field. In particular, giant polarization rotation exceeding 20° has been observed in asymmetric graphene metamolecules, which is further enhanced to almost 40° due the Faraday effect in the applied magnetic field, at around 9 µm wavelength, much higher frequency than the Faraday rotation observed in a semi-infinite graphene microribbons. The results offer a flexible approach for the development of compact, tunable graphene-based photonic devices.