ABSTRACT
Five new α-pyrones, cryptowratones A-E (1-5), and five known congeners (6-10), together with four other known compounds 11-14 were isolated from the twigs of Cryptocarya wrayi. The structures of the new compounds were elucidated on the basis of extensive spectroscopic data analysis and ECD calculations. All α-pyrones except 6 were evaluated for their stimulatory effects on glucose uptake in vitro with CHO-K1/GLUT4 cells. The positive control insulin displayed an approximate 42 ± 0.14% promotion on glucose uptake at 25 µM, compared with the CHO-K1/GLUT4 group. Compounds 1a/2a, 2, 3, and 10 showed a more significant stimulation of glucose uptake than insulin (25 µM) by 36 ± 0.08%, 27 ± 0.12%, 28 ± 0.12%, and 25 ± 0.12% at 1.5 µM, respectively. Immunofluorescence assays indicated the glucose uptake-stimulatory activity of α-pyrones might be correlated with increased GLUT4 translocation.
Subject(s)
Cryptocarya , Cryptocarya/chemistry , Glucose , Molecular Structure , Pyrones/pharmacologyABSTRACT
Two new isoquinoline alkaloids, cryptowrayines A (1) and B (2), along with one known pavine alkaloid (-)-12-hydroxyeschscholtzidine (3), were isolated from the twigs of Cryptocarya wrayi. The structures of new compounds were elucidated by extensive spectroscopic data analysis and electronic circular dichroism (ECD) calculations. Both compounds 1 and 2 exhibited moderate quinone reductase inducing activity in Hepa 1c1c7 cells.
Subject(s)
Alkaloids/isolation & purification , Cryptocarya/chemistry , Isoquinolines/isolation & purification , Alkaloids/chemistry , Alkaloids/metabolism , Glucosidases/antagonists & inhibitors , Glycoside Hydrolase Inhibitors/chemistry , Glycoside Hydrolase Inhibitors/isolation & purification , Glycoside Hydrolase Inhibitors/metabolism , Inhibitory Concentration 50 , Isoquinolines/chemistry , Isoquinolines/metabolism , Magnetic Resonance Spectroscopy , Molecular Structure , NAD(P)H Dehydrogenase (Quinone)/analysis , Optical RotationABSTRACT
A new isoiphionane sesquiterpene, named (3S, 5S, 7S, 10R)-3, 11-dihydroxyisoiphion-4-one (1), two new phloroglucinol glycosides, named eucalglobuside A (2) and eucalglobuside B (3), along with 15 known compounds were isolated from the leaves of Eucalyptus globulus. Their structures were elucidated based on extensive spectroscopic analysis and in comparison with literature data. The absolute configuration of compound 1 was determined by ECD calculation. All isolates were evaluated their inhibitory activities against the mushroom tyrosinase. As a result, three sesquiterpenoids, 1, 5ß, 11-dihydroxy-iphionan-4-one (5), and (-)-globulol (8), exhibited the most potent activities with IC50 values of 14.17⯵M, 10.08⯵M and 9.79⯵M, respectively.
Subject(s)
Enzyme Inhibitors/pharmacology , Eucalyptus/chemistry , Glycosides/pharmacology , Monophenol Monooxygenase/antagonists & inhibitors , Phloroglucinol/pharmacology , Sesquiterpenes/pharmacology , China , Enzyme Inhibitors/isolation & purification , Glycosides/isolation & purification , Molecular Structure , Phloroglucinol/isolation & purification , Phytochemicals/isolation & purification , Phytochemicals/pharmacology , Plant Leaves/chemistry , Sesquiterpenes/isolation & purificationABSTRACT
Four new prenylated 2-arylbenzofurans, namely artopithecins A-D (1-4), together with five known compounds (5-9) were isolated from the twigs of Artocarpus pithecogallus for the first time. Their structures were elucidated based on extensive spectroscopic analysis and in comparison with literature data. All isolates were evaluated for their inhibitory activities against mushroom tyrosinase. Compounds 3 and 4 displayed significant tyrosinase inhibitory activities with IC50 values of 37.09±0.33 and 38.14±0.21 µM, respectively.
Subject(s)
Artocarpus/chemistry , Benzofurans/pharmacology , Enzyme Inhibitors/pharmacology , Monophenol Monooxygenase/antagonists & inhibitors , Plant Stems/chemistry , Agaricales/enzymology , Benzofurans/chemistry , Benzofurans/isolation & purification , Dose-Response Relationship, Drug , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/isolation & purification , Molecular Structure , Monophenol Monooxygenase/metabolism , Prenylation , Structure-Activity RelationshipABSTRACT
Two new prenylated flavones, artocarnin A (2) and carpachromenol (12), together with 13 known prenylflavonoids (1, 3-11, 13-15) were isolated from the twigs of Artocarpus nigrifolius for the first time. Their structures were elucidated by high resolution-electrospray ionization (HR-ESI)-MS, NMR spectroscopic analysis, and in comparison with the reported data. Compounds 1-15 were evaluated for their antiproliferative effects against SiHa and SGC-7901 human cancer cell lines in vitro. The most active compound, eleocharin A (10), showed significant cytotoxicity on SiHa cells (IC50=0.7±0.1 µM) and inhibitory activity against SGC-7901 cells (IC50=8.3±0.2 µM) and could be considered as potential lead compound for further development of novel anti-tumor agents.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Artocarpus/chemistry , Flavonoids/pharmacology , Plant Bark/chemistry , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Cell Proliferation/drug effects , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Flavonoids/chemistry , Flavonoids/isolation & purification , Humans , Molecular Structure , Structure-Activity Relationship , Tumor Cells, CulturedABSTRACT
Five new guaiane-type sesquiterpenoids, hedyosumins A-E (1-5), together with five known ones (6-10), were isolated from the aerial parts of Hedyosmum orientale. Two known sesquiterpenoids, 10alpha-hydroxy-1,5alpha H-guaia-3,7(11)-dien-8alpha,12-olide and 9alpha-hydroxyasterolide, were obtained as natural products for the first time. Their structures were elucidated on the basis of spectroscopic methods. 9alpha-Hydroxyasterolide (7) showed moderate activities against A-549 and HL-60 tumor cell lines with the IC 50 values of 3.1 and 8.8 microM, respectively.
Subject(s)
Magnoliopsida/chemistry , Sesquiterpenes/isolation & purification , Cell Line, Tumor , Chromatography, High Pressure Liquid , Humans , Magnetic Resonance Spectroscopy , Molecular Structure , Sesquiterpenes/chemistry , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, UltravioletABSTRACT
Five new diterpenes, trigonochinenes A-E (1-5), and two known ones, 3,4- seco-sonderianol (6) and 3,4- seco-sonderianic acid (7), were isolated from the aerial part of Trigonostemon chinensis. Compounds 1-4 possess a rare 3,4-seco-cleistanthanic skeleton, and compound 5 is a highly aromatized tetranorditerpene. Structures of these compounds were elucidated by spectroscopic analysis. The antimicrobial activities of compounds 1-7 were evaluated against a panel of bacteria and fungi.
Subject(s)
Anti-Infective Agents/isolation & purification , Diterpenes/isolation & purification , Euphorbiaceae/chemistry , Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Bacteria/drug effects , Circular Dichroism , Diterpenes/chemistry , Diterpenes/pharmacology , Fungi/drug effects , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Molecular Structure , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, InfraredABSTRACT
Walsucochins A (1) and B (2) with an unprecedented skeleton were isolated from Walsura cochinchinensis. Their structures including absolute configuration were elucidated by spectral methods. A biosynthetic pathway of 1 and 2 was postulated. Both 1 and 2 exhibited significant cell protecting activities against H2O2-induced PC12 cell damage.
