ABSTRACT
PURPOSE: To evaluate the effect of Mtwo NiTi files in the management of calcified root canals. METHODS: Thirty-five calcified root canals from 21 cases unsuccessfully prepared with C-Pilot files and EDTA were treated with Mtwo NiTi files and EDTA. The data was analyzed with SAS 9.4 software package. RESULTS: Twenty-three calcified root canals were successfully prepared. The success rate was 65.71%. Instrument separation occurred in 4 root canals, and ledge occurred in 3 root canals. Categorized by calcified position, the success rate of three groups had no significant differences (P>0.05). CONCLUSION: Mtwo NiTi files can effectively process calcified root canals.
Subject(s)
Dental Pulp Cavity , Root Canal Preparation , Humans , Nickel , Root Canal Therapy , TitaniumABSTRACT
INTRODUCTION: In previous studies, we found that hypoxia promoted the mineralization of dental pulp cells (DPCs). However, the clinical application of hypoxia as a therapy is questionable or unfeasible. Deferoxamine (DFO), a medication for iron overload, has also been shown to induce hypoxia. The purpose of this study was to investigate the effects of DFO on the repair ability of DPCs. METHODS: DPCs were obtained by using a tissue explant technique in vitro and were treated with different concentrations of DFO or hypoxia culture for 2 days. The viability, proliferation, migration, and odontogenic differentiation of DPCs were assayed and analyzed. The expression of hypoxia-inducible factor 1-alpha (HIF-1α) was assessed through Western blotting. RESULTS: Ten micromolars of DFO enhanced the expression of HIF-1α similarly to hypoxia and did not affect the viability of DPCs for 2 days. Furthermore, the proliferation, migration, and odontogenic differentiation of DPCs were promoted by DFO. CONCLUSIONS: These results suggest that DFO might improve the repair ability of DPCs by HIF-1α.
Subject(s)
Cell Hypoxia/drug effects , Deferoxamine/pharmacology , Dental Pulp/cytology , Siderophores/pharmacology , Adolescent , Alkaline Phosphatase/analysis , Calcification, Physiologic/drug effects , Cell Culture Techniques , Cell Differentiation/drug effects , Cell Movement/drug effects , Cell Proliferation/drug effects , Cell Survival/drug effects , Cells, Cultured , Coloring Agents , Dental Pulp/drug effects , Fluorescent Dyes , Humans , Hypoxia-Inducible Factor 1, alpha Subunit/analysis , Odontoblasts/drug effects , Osteocalcin/analysis , Regeneration/drug effects , Tetrazolium Salts , Thiazoles , Young AdultABSTRACT
PURPOSE: To investigate the different incidence of postoperative pain and long-term follow-up curative effects in patients with chronic periapical periodontitis undergoing single visit root canal treatment by two kinds of canal preparation instruments system. METHODS: Ninety-eight permanent teeth with chronic periapical periodontitis were divided into two groups randomly. One group was prepared with nickel-titanium instruments, the other group was prepared with K files. After canal preparation, all the teeth underwent canal filling immediately. The different incidence of postoperative pain and long-term follow-up curative effects were recorded. Statistical analysis was carried out using SPSS13.0 software package. RESULTS: One week after treatment, the rate of serious symptom of periapical periodontitis in the Ni-Ti group was less than that in the K file group (P<0.05 ). 18 months after treatment, the curative effect of two groups had no significant difference (P>0.05 ). CONCLUSIONS: Nickel-titanium instrument used in treatment of chronic periapical periodontitis lead to less serious postoperative reaction than K file instruments, but the same long-term follow-up curative effects as K file instruments. Nickel-titanium instruments system is worthy of wide application in patients with chronic periapical periodontitis undergoing single visit root canal treatment.
