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1.
Sci Adv ; 9(1): eadc8917, 2023 Jan 04.
Article in English | MEDLINE | ID: mdl-36598980

ABSTRACT

Although excessive lipid accumulation is a hallmark of obesity-related pathologies, some lipids are beneficial. Oleic acid (OA), the most abundant monounsaturated fatty acid (FA), promotes health and longevity. Here, we show that OA benefits Caenorhabditis elegans by activating the endoplasmic reticulum (ER)-resident transcription factor SKN-1A (Nrf1/NFE2L1) in a lipid homeostasis response. SKN-1A/Nrf1 is cleared from the ER by the ER-associated degradation (ERAD) machinery and stabilized when proteasome activity is low and canonically maintains proteasome homeostasis. Unexpectedly, OA increases nuclear SKN-1A levels independently of proteasome activity, through lipid droplet-dependent enhancement of ERAD. In turn, SKN-1A reduces steatosis by reshaping the lipid metabolism transcriptome and mediates longevity from OA provided through endogenous accumulation, reduced H3K4 trimethylation, or dietary supplementation. Our findings reveal an unexpected mechanism of FA signal transduction, as well as a lipid homeostasis pathway that provides strategies for opposing steatosis and aging, and may mediate some benefits of the OA-rich Mediterranean diet.

2.
Hum Gene Ther ; 34(1-2): 42-55, 2023 01.
Article in English | MEDLINE | ID: mdl-36373826

ABSTRACT

Histone H3 is a nucleosome scaffold protein that is involved in a variety of intracellular processes. Aberrant modification of H3 is important in carcinogenesis. In contrast, free histones in cells can act as stimuli to trigger cellular immune responses and cell death. In this study, we linked cell-penetrating peptide HIV Tat to a histone H3 fragment to achieve intracellular delivery in tumor cells. We found that Tat-conjugated histone polypeptides localized to nuclei of lung and breast cancer cells and caused cell death. A trans-configured Tat sequence displayed dramatically improved peptide half-life and cytotoxicity. Mechanistic studies demonstrated that treatment with the peptides significantly elevated mitogen-activated protein kinase (MAPK) signaling, reactive oxygen species (ROS) production, as well as levels of stress-inducible transcription factor ATF3 (activating transcription factor 3) and AP-1 (activating protein-1). Cytotoxicity of the peptide was significantly reduced by inhibition of AP-1 activity and ROS production. These results suggest the potential of Tat-conjugated H3 peptides as antitumor agents to induce cell death via increased cellular stress response by activating p38-MAPK signaling and intracellular ROS production.


Subject(s)
HIV Infections , Histones , Humans , Histones/metabolism , Reactive Oxygen Species/metabolism , Transcription Factor AP-1/metabolism , Cell Death , Peptides/pharmacology
3.
Cell Death Dis ; 11(6): 459, 2020 06 15.
Article in English | MEDLINE | ID: mdl-32541671

ABSTRACT

Macrophages play critical roles in the first-line immune defense against airway infections caused by Pseudomonas aeruginosa (PA). The redox-active phenazine-pyocyanin (PCN), as one of the most essential virulence factors, facilities PA-related infection via a wide spectrum of cellular oxidative damages. However, little is known for PCN cytotoxicity in macrophages. In this study, besides showing PCN-mediated reactive oxygen species (ROS) indeed involved in macrophage viability and function impairment, we at the first time demonstrated a novel role of reactive nitrogen species (RNS) pathway causing cellular damage in PCN-challenged macrophages. Using small molecule inhibitor JQ1 targeting Bromodomain and extra-terminal family proteins, we showed restrained iNOS-dependent nitric oxide (NO) production correlated with abolished Brd4 recruitment to the NOS2 (encoding inducible nitric oxide synthase-iNOS) promoter. Application of JQ1 diminished PCN-mediated peroxynitrite (ONOO-) that followed ROS and NO induction, restored macrophage survival and bacteria clearance as well as repressed local inflammation in PA/PCN-challenged mice lungs. Our results uncover a novel link between PCN-mediated macrophage dysfunction and reactive free radicals that rely on Brd4-dependent transcription modulation of multiple stress-response genes, suggesting Brd4 could be a promising therapeutic target in treating PA-related lung infection.


Subject(s)
Cell Cycle Proteins/antagonists & inhibitors , Free Radicals/metabolism , Macrophages/drug effects , Nitrogen/metabolism , Pyocyanine/adverse effects , Reactive Oxygen Species/metabolism , Transcription Factors/antagonists & inhibitors , Animals , Humans , Male , Mice
4.
Biochem Biophys Res Commun ; 513(1): 193-200, 2019 05 21.
Article in English | MEDLINE | ID: mdl-30952427

ABSTRACT

Urinary tract infection is one of the most common bacterial infections which is mainly caused by Escherichia coli (UPEC). Autophagy plays a key role in immune response to eliminate invading pathogens. Exploring the effect of autophagy on UPEC infection and the molecular mechanisms will be benefit for the treatment of urinary tract infection. High-mobility group protein N2 (HMGN2), a highly conserved nuclear protein and an antibacterial peptide, has been associated with bacterial infection induced immune response; however, whether this function is due to the regulation of autophagy remains unclear. In this study, we demonstrate for the first time that HMGN2 is upregulated in UPEC infection of bladder epithelial cell line 5637 (BEC 5637). Furthermore, HMGN2 enhances autophagy in BEC 5637 via activation of AMPK and ULK1, whereas UPEC suppresses autophagy. In addition, the enhanced autophagy activity by HMGN2 overexpression or rapamycin boosts the proliferation of UPEC J96 in BEC 5637. In summary, our data indicate that HMGN2 activates autophagy via AMPK/ULK1 pathway which can be utilized by UPEC J96 for their proliferation within bladder epithelial cells.


Subject(s)
AMP-Activated Protein Kinases/metabolism , Autophagy-Related Protein-1 Homolog/metabolism , Escherichia coli Infections/metabolism , HMGN2 Protein/metabolism , Urinary Bladder/microbiology , Urinary Tract Infections/metabolism , Animals , Autophagy , Cell Line , Cell Proliferation , Epithelial Cells/cytology , Epithelial Cells/metabolism , Epithelial Cells/microbiology , Escherichia coli/growth & development , Escherichia coli/metabolism , Escherichia coli Infections/microbiology , Female , Humans , Mice, Inbred C57BL , Signal Transduction , Urinary Bladder/cytology , Urinary Bladder/metabolism , Urinary Tract Infections/microbiology
5.
J Biol Chem ; 294(8): 2616-2627, 2019 02 22.
Article in English | MEDLINE | ID: mdl-30598505

ABSTRACT

α4 integrin plays a crucial role in retention and release of neutrophils from bone marrow. Although α4 integrin is known to be a potential target of reactive oxygen species (ROS)-induced cysteine glutathionylation, the physiological significance and underlying regulatory mechanism of this event remain elusive. Here, using in vitro and in vivo biochemical and cell biology approaches, we show that physiological ROS-induced glutathionylation of α4 integrin in neutrophils increases the binding of neutrophil-associated α4 integrin to vascular cell adhesion molecule 1 (VCAM-1) on human endothelial cells. This enhanced binding was reversed by extracellular glutaredoxin 1 (Grx1), a thiol disulfide oxidoreductase promoting protein deglutathionylation. Furthermore, in a murine inflammation model, Grx1 disruption dramatically elevated α4 glutathionylation and subsequently enhanced neutrophil egress from the bone marrow. Corroborating this observation, intravenous injection of recombinant Grx1 into mice inhibited α4 glutathionylation and thereby suppressed inflammation-induced neutrophil mobilization from the bone marrow. Taken together, our results establish ROS-elicited glutathionylation and its modulation by Grx1 as pivotal regulatory mechanisms controlling α4 integrin affinity and neutrophil mobilization from the bone marrow under physiological conditions.


Subject(s)
Bone Marrow/metabolism , Glutaredoxins/metabolism , Integrin alpha4/metabolism , Neutrophils/metabolism , Up-Regulation , Vascular Cell Adhesion Molecule-1/metabolism , Animals , Bone Marrow/pathology , Disease Models, Animal , Glutaredoxins/genetics , HL-60 Cells , Humans , Inflammation/genetics , Inflammation/metabolism , Inflammation/pathology , Integrin alpha4/genetics , Mice, Knockout , Neutrophils/pathology , Vascular Cell Adhesion Molecule-1/genetics
6.
Acta Biochim Pol ; 65(1): 93-100, 2018.
Article in English | MEDLINE | ID: mdl-29549670

ABSTRACT

The urinary tract is vulnerable to frequent challenges from environmental microflora. Uropathogenic Escherichia coli (UPEC) makes a major contribution to urinary tract infection (UTI). Previous studies have characterized positive roles of non-histone nuclear protein HMGN2 in lung epithelial innate immune response. In the study presented here, we found HMGN2 expression was up-regulated in UPEC J96-infected urothelium. Surprisingly, over-expression of HMGN2 promoted disruption of BECs 5637 cells' intercellular junctions by down-regulating tight junction (TJs) components' expression and physical structure under J96 infection. Further investigation showed that BECs 5637 monolayer, in which HMGN2 was over-expressed, had significantly increased permeability to J96. Our study systemically explored the regulatory roles of HMGN2 in BECs barrier function during UPEC infection and suggested different modulations of intracellular and paracellular routes through which UPEC invades the bladder epithelium.


Subject(s)
Antimicrobial Cationic Peptides/metabolism , HMGN2 Protein/physiology , Tight Junction Proteins/metabolism , Urothelium/microbiology , Epithelial Cells/metabolism , HMGN2 Protein/genetics , Humans , Up-Regulation , Urinary Bladder/cytology , Urinary Bladder/pathology , Urinary Tract Infections/microbiology , Uropathogenic Escherichia coli/pathogenicity , Urothelium/cytology , Urothelium/physiology
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