Characterization of soundscape assessment in outdoor public spaces of urban high-rise residential communitiesa).

Soundscape perceptual models were developed in various contexts. However, as the outdoor public space in high-rise residential communities differs in terms of space planning and management, the soundscape perceptual characteristics are still unclear. In this study, an on-site survey was conducted to obtain the perceptual dimensions of soundscape in outdoor public spaces in urban high-rise residential communities based on evaluations of residents. Meantime, the soundscape of the space in different community layouts were compared. It was found that: (1) Four dimensions of outdoor soundscape in high-rise communities were extracted, namely Relaxation, Communication, Quietness, and Spatiality. The first three dimensions were positively correlated with overall soundscape satisfaction significantly. (2) Relaxation was mostly correlated with dominance of noise; Communication and Quietness were primarily related to sounds from human beings. (3) Lower traffic noise and higher levels of human sounds were perceived in enclosed communities, resulting in higher Relaxation and lower Quietness scores in outdoor public spaces in enclosed communities than non-enclosed ones. These findings evoke insights into the understanding of soundscape assessment in different contexts, and provide implications for sound environment design in urban high-rise communities.

The association between type 2 diabetes and pulmonary cavitation revealed among IGRA-positive tuberculosis patients.

The co-occurrence of tuberculosis (TB) and diabetes mellitus (DM) presents a significant obstacle to TB eradication. Pulmonary cavitation can occur in severe cases of TB, particularly in patients with DM. From 1 May 2014 through 30 June 2019, we conducted a cross-sectional study of 1,658 smear- or culture-confirmed pulmonary TB (PTB) patients at the Second Department of Pulmonary Medicine and Tuberculosis, Shenzhen, China. A total of 861 participants who satisfied the criteria (chest CT scan for cavitation, interferon-gamma release assay (IGRA), diagnosis of diabetes mellitus), with the median age of 36.7 years, 63.6% of male, 79.7% IGRA positive, 13.8% with diabetes, and 40.8% with pulmonary cavitation, were included in the study. The association between diabetes and pulmonary cavitation was confirmed in these TB patients (adjusted OR, 2.54; 95% CI, 1.66-3.94; p < 0.001). No associations were observed between diabetes and IGRA, as well as between lung cavitary and IGRA. Based on the criteria of IGRA+/-, pulmonary cavitation+/-, and DM+/-, the further analysis with univariate and multivariate logistic regression were conducted in six subgroups. The significant association between diabetes and pulmonary cavitation was further confirmed in the IGRA+ subgroup (adjusted OR, 3.07; 95% CI, 1.86-5.16; p < 0.001) but not observed in IGRA- individuals. This observation suggests that different immunological mechanisms of pulmonary cavitary/DM may be employed in IGRA+ TB patients from IGRA- TB patients.

Creating a near-perfect circularly polarized terahertz beam through the nonreciprocity of a magnetoplasma.

Compared to other parts of the electromagnetic spectrum, the terahertz frequency range lacks efficient polarization manipulation techniques, which is impeding the proliferation of terahertz technology. In this work, we demonstrate a tunable and broadband linear-to-circular polarization converter based on an InSb plate containing a free-carrier magnetoplasma. In a wide spectral region (∼ 0.45 THz), the magnetoplasma selectively absorbs one circularly polarized mode due to electron cyclotron resonance and also reflects it at the edges of the absorption band. Both effects are nonreciprocal and contribute to form a near-zero transmission band with a high isolation of -36 dB, resulting in the output of a near-perfect circularly polarized terahertz wave for an incident linearly polarized beam. The near-zero transmission band is tunable with magnetic field to cover a wide frequency range from 0.3 to 4.8 THz.

Quizartinib inhibits necroptosis by targeting receptor-interacting serine/threonine protein kinase 1.

Systemic inflammatory response syndrome (SIRS), at least in part driven by necroptosis, is characterized by life-threatening multiple organ failure. Blocking the progression of SIRS and consequent multiple organ dysfunction is challenging. Receptor-interacting serine/threonine protein kinase 1 (RIPK1) is an important cell death and inflammatory mediator, making it a potential treatment target in several diseases. Here, using a drug repurposing approach, we show that inhibiting RIPK1 is also an effective treatment for SIRS. We performed cell-based high-throughput drug screening of an US Food and Drug Administration (FDA)-approved drug library that contains 1953 drugs to identify effective inhibitors of necroptotic cell death by SYTOX green staining. Dose-response validation of the top candidate, quizartinib, was conducted in two cell lines of HT-22 and MEFs. The effect of quizartinib on necroptosis-related proteins was evaluated using western blotting, immunoprecipitation, and an in vitro RIPK1 kinase assay. The in vivo effects of quizartinib were assessed in a murine tumor necrosis factor α (TNFα)-induced SIRS model. High-throughput screening identified quizartinib as the top "hit" in the compound library that rescued cells from necroptosis in vitro. Quizartinib inhibited necroptosis by directly inhibiting RIPK1 kinase activity and blocking downstream complex IIb formation. Furthermore, quizartinib protected mice against TNFα-induced SIRS. Quizartinib, as an FDA-approved drug with proven safety and efficacy, was repurposed for targeted inhibition of RIPK1. This work provides essential preclinical data for transferring quizartinib to the treatment of RIPK1-dependent necroptosis-induced inflammatory diseases, including SIRS.

Identification of Piperlongumine as Potent Inhibitor of Necroptosis.

Purpose: Excessive necroptosis contributes to the pathogenesis of several inflammatory and neurodegenerative diseases. Here, using a high-throughput screening approach, we investigated the anti-necroptosis effects of piperlongumine, an alkaloid isolated from the long pepper plant, in vitro and in a mouse model of systemic inflammatory response syndrome (SIRS). Methods: A natural compound library was screened for anti-necroptosis effects in cellular. The underlying mechanism of action of the top candidate piperlongumine was explored by quantifying the necroptosis marker phosphorylated receptor-interacting protein kinase 1 (p-RIPK1) by Western blotting. The anti-inflammatory effect of piperlongumine was assessed in a tumor necrosis factor α (TNFα)-induced SIRS model in mice. Results: Among the compounds investigated, piperlongumine significantly rescued cell viability. The half maximal effective concentration (EC50) of piperlongumine for inhibiting necroptosis was 0.47 µM in HT-29 cells, 6.41 µM in FADD-deficient Jurkat cells, and 2.33 µM in CCRF-CEM cells, while the half maximal inhibitory concentration (IC50) was 95.4 µM in HT-29 cells, 93.02 µM in FADD-deficient Jurkat cells, and 161.1 µM in CCRF-CEM cells. Piperlongumine also significantly inhibited TNFα-induced intracellular RIPK1 Ser166 phosphorylation in cell lines and significantly prevented decreases in body temperature and improved survival in SIRS mice. Conclusion: As a potent necroptosis inhibitor, piperlongumine prevents phosphorylation of RIPK1 at its activation residue Ser166. Piperlongumine thus potently inhibits necroptosis at concentrations safe enough for human cells in vitro and inhibits TNFα-induced SIRS in mice. Piperlongumine has potential clinical translational value for the treatment of the spectrum of diseases associated with necroptosis, including SIRS.

Restorative effect of audio and visual elements in urban waterfront spaces.

Introduction: Urban waterfront spaces are often composed of built infrastructures and nature elements. Though citizens could take advantage of these public spaces to relax from daily work, its restorative potential has not been paid enough attention. In this study, the restorative effect and mechanism of different audio and visual elements in urban waterfront spaces was systematically studied. Methods: At the first stage, restorative potential of waterfront spaces was investigated and different elements with restorative effects were identified through an on-site survey, in which visual and auditory forms of environmental-nature, animal-nature, on-water human activities and on-shore human activities were identified. At the second stage, a series of laboratory experiments were conducted to explore the restorative function of the audio and visual elements. Results and discussion: It is found that the degree of artificiality of waterfront space was a crucial factor influencing the restoration level of the space, and higher artificiality level of waterfront space resulted in lower level of perceived restoration. However it was available by adding visual and audio elements to the scene to facilitate the restorative effect in waterfront spaces with high-level artificiality. The effects of adding visual and auditory elements on psychophysiological restoration were explored, and elements that should be recommended and restrained were discussed. Prospects: These findings would provide applicable suggestions for future design and rebuilding of urban waterfront spaces.

Distribution of soil organic carbon and carbon sequestration potential of different geomorphic units in Shiyang river basin, China.

Accurate assessment of soil C storage patterns and control factors on a regional and global scale is essential for predicting and mitigating soil C feedback to global environmental change. We used soil samples collected in the Shiyang River Basin in 2018, combined with remote sensing data, climate and meteorological data, watershed hydrological data, and soil physical and chemical properties to discuss the change characteristics and influencing factors of soil organic carbon (SOC) under different soil depths in the Shiyang River Basin in an arid area and analyze the storage model and carbon sequestration potential of soil organic carbon in different geomorphic units. The research results show that, (1) in spatial distribution, the SOC content in the Shiyang River Basin shows an obvious regional difference, and the average content of SOC in the oasis area in the middle reaches significantly higher than that in the mountain area in the upstream and desert area in the downstream. In vertical distribution, the content of SOC in the whole watershed decreases with the soil depth increase. (2) Soil carbon sequestration potential decreased with the increase in soil depth, but there were regional differences. The Oasis area in the middle reaches of the Shiyang River Basin is a high-potential area. In contrast, most of the upper mountain areas and the lower reaches of the desert area are low-potential areas. Environmental factors such as vegetation cover, meteorological factors, and physical and chemical properties of soil are important factors that promote the spatial variability of SOC content. The decisive effect of environmental factors on the SOC content is most significant in the surface layer 0-20 cm.

Repurposing crizotinib to target RIPK1-dependent cell death.

Receptor-interacting protein kinase 1 (RIPK1) has emerged as a key regulator of cell death and inflammation, which are implicated in the pathogenesis of many inflammatory and degenerative diseases. RIPK1 is therefore a putative therapeutic target in many of these diseases. However, no pharmacological inhibitor of RIPK1-mediated cell death is currently in clinical use. Recognizing that a repurposed drug has an expedited clinical development pipeline, here we performed a high-throughput drug screen of Food and Drug Administration (FDA)-approved compounds and identified a novel use for crizotinib as an inhibitor of RIPK1-dependent cell death. Furthermore, crizotinib rescued TNF-α-induced death in mice with systemic inflammatory response syndrome. RIPK1 kinase activity was directly inhibited by crizotinib. These findings identify a new use for an established compound and are expected to accelerate drug development for RIPK1-spectrum disorders.

Effects of agricultural activities on hydrochemistry in the Shiyang River Basin, China.

Agricultural water accounts for more than 80% of the available water in arid areas. Agricultural activities have a great impact on surface water and groundwater. If the impact of agricultural activities on hydrochemistry is not prevented, the risk of water quality change in arid areas may be greatly intensified. Based on the hydrochemical data of the whole Shiyang River Basin from April 2014 to October 2019, this paper analyzes the impact of agricultural activities on hydrochemistry in the basin. The results show that (i) in the middle and lower reaches of farmland with high intensity of agricultural activities, the ion concentration of groundwater in summer and autumn is significantly higher than that in winter and spring due to the influence of irrigation; (ii) the runoff ion concentration in the backflow of the river reaches recharged by irrigation water is significantly higher than that of other reaches; (iii) due to strong evaporation, different types of reservoirs will lead to an overall increase in ion concentration, which is more obvious in plain reservoirs and river tail lakes. In addition, the reservoirs have a certain removal effect on nitrates.

Hispidulin Enhances Temozolomide (TMZ)-Induced Cytotoxicity against Malignant Glioma Cells In Vitro by Inhibiting Autophagy.

Temozolomide (TMZ), an oral alkylating agent, is the widely used first-line chemotherapeutic reagent for glioma in clinical practice. However, TMZ-induced autophagy is another cellular process favoring glioma cell survival. This study aimed to explore whether hispidulin can facilitate TMZ-induced cell death of glioma. The MTT assay showed that coadministration with hispidulin and TMZ could significantly decrease the viability of glioma U87MG cells. Meanwhile, hispidulin administration was also observed to promote TMZ-induced apoptosis. Furthermore, additional hispidulin treatment further elevated TMZ-induced expression of Bax, cleaved-caspase-9, and cleaved-caspase-3 protein but decreased Bcl-2 protein expression in U87MG cells. We also observed that hispidulin suppressed TMZ-induced autophagy to promote apoptosis, as showed by decreased AVOs and LC3B-I/II protein expression. These results collectively suggested that the combination of hispidulin and TMZ could improve the antitumor efficiency of TMZ against malignant gliomas.

OPTN variants in ALS cases: a case report of a novel mutation and literature review.

INTRODUCTION: Optineurin (OPTN)-associated mutations have been implicated in the development of type 12 amyotrophic lateral sclerosis (ALS12). We reported a case of ALS with a new OPTN variant (p.D527fs) and reviewed relevant literature to better understand the phenotypes and pathophysiological mechanisms of ALS12. METHODS: We report a case of a 55-year-old female patient with a new heterozygous variant of the OPTN gene. A literature search of ALS cases associated with the OPTN gene mutations was performed in PubMed with the search criteria as [("amyotrophic lateral sclerosis") OR ("motor neuron disease")] AND ("OPTN"). RESULTS: The case of ALS with a new OPTN variant (p.D527fs) in our report manifested with bulbar involvement in onset and a rapidly progressive course. A literature review of 37 ALS patients with OPTN mutations included 20 males and 16 females with another patient whose gender was not described. The mean onset age of 37 ALS12 patients was 48 with the youngest 23 and the oldest 83 years old. Differences in onset age between male and female patients were not significant. Mean time from initiation to death was 61.8 ± 12.0 months. Patients present with either limb onset (73.5% cases) or bulbar onset (23.5% cases). CONCLUSION: Through the literature review, we summarized the clinical characteristics of ALS12. The phenotypes of the reported patients elucidate the genetic profiles and clinical phenotypes of ALS12. Clinicians should pay close attention to the role of receptor-interacting kinase 1 (RIPK1)-dependent necroptosis in the pathophysiologic development of ALS12, since necroptosis inhibitors are expected as potential therapeutic agents for treating ALS12.

Reverse design of pixel-type terahertz band-pass filters.

Reverse design is a frontier direction in the optical research field. In this work, reverse design is applied to the design of terahertz devices. We have employed direct binary search (DBS) and binary particle swarm optimization (BPSO) algorithms to design pixel-type terahertz band-pass filters, respectively. Through a comparative analysis of the designed devices, we found that BPSO algorithm converged faster than DBS algorithm, and the device performance is better on out-of-band suppression. We have fabricated a sample utilizing femtosecond laser micromachining and characterized it by terahertz time-domain spectroscopy. The experimental results were consistent with the finite difference time domain (FDTD) simulation. Our method can simultaneously optimize multiple characteristics of the band-pass filters, including the peak transmittance, out-of-band transmittance, bandwidth, and polarization stability, which can not be achieved by traditional optical design methods.

Contribution of recycled moisture to precipitation and its influencing factors in the subalpine zone of Qilian Mountains.

Recycled moisture, the part of precipitating vapor that comes from surface evapotranspiration, plays an important role in the hydrological process and the feedback of the land surface-atmosphere system. In this study, we use the δ2H and δ18O data of precipitation, plant water, and surface water systematically collected in the subalpine area of the Qilian Mountains from May to October, 2019, and assess the contribution of recycled moisture to precipitation based on the three-component isotopic mixing model. The results indicated that the main source of precipitating vapor comes from the advected vapor, and its contribution reaches up to 87.8 ± 1.6%. The contribution of local recycled moisture is 12.2 ± 1.6%, in which the proportion of transpiration and evaporation are 8.9 ± 0.6% and 3.3 ± 1.0%, respectively. In terms of the correlation between recycled moisture and its influencing factors, the temperature has a positive effect on evapotranspiration and promotes moisture recycling, while precipitation and humidity have a certain inhibition effect on evapotranspiration and reduce the contribution of recycled moisture.

Reverse Design of On-Chip Terahertz Demultiplexers.

The reverse design method (RDM) is a frontier direction in the optical research field. In this work, RDM is applied to the design of terahertz demultiplexers, including two-port and three-port terahertz demultiplexers, with areas of 3 mm × 3 mm and 5 mm × 5 mm, respectively. The Finite-Difference Time-Domain (FDTD) simulation results show that the terahertz waves at frequencies of 0.5 THz and 0.417 THz can be well separated by the two-port demultiplexer, and the transmittances of the two outputs reach as high as 0.75 after bandwidth optimization. Meanwhile, the three-port terahertz demultiplexer can have terahertz waves separated from three Ports, and the crosstalk between adjacent channels is less than -18 dB.

Background subtraction for night videos.

Motion analysis is important in video surveillance systems and background subtraction is useful for moving object detection in such systems. However, most of the existing background subtraction methods do not work well for surveillance systems in the evening because objects are usually dark and reflected light is usually strong. To resolve these issues, we propose a framework that utilizes a Weber contrast descriptor, a texture feature extractor, and a light detection unit, to extract the features of foreground objects. We propose a local pattern enhancement method. For the light detection unit, our method utilizes the finding that lighted areas in the evening usually have a low saturation in hue-saturation-value and hue-saturation-lightness color spaces. Finally, we update the background model and the foreground objects in the framework. This approach is able to improve foreground object detection in night videos, which do not need a large data set for pre-training.

GLCCI1 reduces collagen deposition and airway hyper-responsiveness in a mouse asthma model through binding with WD repeat domain 45B.

Asthma is a serious public health problem worldwide, without effective therapeutic methods. Our previous study indicated that glucocorticoid-induced transcript 1 gene (GLCCI1) knockout reduces the sensitivity to glucocorticoid in asthmatic mouse. Here, we explored the role and action mechanism of GLCCI1 in asthma development. In ovalbumin-sensitized mice, airway resistance and tissue damage increased, the production of inflammatory cytokines were up-regulated, GLCCI1 expression was reduced and autophagy was activated. Increasing of GLCCI1 inhibited human and mouse airway epithelial cell (AEC) autophagy, while decreasing of GLCCI1 promoted autophagy. Furthermore, we found that GLCCI1 bound with WD repeat domain 45B (WDR45B) and inhibited its expression. Increasing of WDR45B partly reversed the inhibition of GLCCI1 to autophagy-related proteins expression and autophagosome formation in vitro. Increasing of WDR45B in vivo reversed the improvement of GLCCI1 on airway remodelling in asthma and the inhibition to autophagy level in lung tissues. Overall, our data showed that GLCCI1 improved airway remodelling in ovalbumin-sensitized mice through inhibiting autophagy via combination with WDR45B and inhibiting its expression. Our results proved a new idea for asthma treatment.

Ion migration process and influencing factors in inland river basin of arid area in China: a case study of Shiyang River Basin.

A thorough understanding of the processes and driving factors of ion migration, dilution, and enrichment in arid inland river basins is the basis for implementing water resources management. In this study, we analyzed the water chemistry of streamflow, groundwater, and precipitation and the behavior of main elements in the Shiyang River Basin by means of the hydrochemical diagram and multivariate statistical analysis. The spatial variation of water chemistry was obvious, and the conversion between different water bodies was frequent. The ions migrated from the mountain area to the oasis and desert and accumulated near the terminal lake finally. There were obvious differences in hydrochemistry between surface water and groundwater. From the mountain to the basin, the hydrochemical type of surfer water has varied, and the hydrochemical type of groundwater has changed from Ca-Cl type to Na-Cl type. The hydrochemistry of the basin was controlled by silicate weathering. However, the influence of water-rock interaction on surface water and groundwater was different, and the surface water was more complex. Significantly, agricultural activities and sewage discharge had a negative impact on the water environment. Interbasin water transfer (IBWT) was a form of external ions input from outside the basin, which affected the chemical characteristics of surface water in the lower reaches to a certain extent. In arid areas, human impact on water chemistry needs to be paid attention. These results are helpful to strengthen the understanding of the relationship between different regions and different water bodies in the arid basin.

Glucocorticoid induced transcript 1 represses airway remodeling of asthmatic mouse via inhibiting IL-13/periostin/TGF-ß1 signaling.

Asthma is characterized by airway remodeling. Glucocorticoid induced transcript 1 (GLCCI1) was reported to be associated with the development of asthma, while its exact mechanism is still not clear. In our study, ovalbumin (OVA) combined with aluminum hydroxide were used to establish asthmatic mouse model. ELISA assay was fulfilled to ensure the concentration of inflammatory factors in both bronchoalveolar lavage fluid and serum. The pathological changes and collagen deposition in lung tissues were analyzed using H&E staining and Masson staining, respectively. The expression of proteins was measured using western blot, and the expression of GLCCI1 mRNA was ensured by qRT-PCR. Here, we demonstrated that OVA-induced inflammation, lung structural remodeling and collagen deposition in asthmatic mice was notably improved by hydroprednisone treatment or GLCCI1 overexpressing. The expression of GLCCI1 was decreased, while IL-13, periostin and TGF-ß1 were increased in the lung tissue of asthmatic mice. Importantly, upregulation of GLCCI1 suppressed the expression of IL-13, periostin and TGF-ß1, phosphorylation of Smad2 and Smad3, and extracellular matrix (ECM) deposition-related proteins expression. IL-13-induced upregulation of periostin and TGF-ß1 expression, phosphorylation of Smad2 and Smad3, and ECM deposition in airway epithelial cells (AECs) was repressed by GLCCI1 increasing. Furthermore, our results showed that overexpression of GLCCI1 repressed the effect of IL-13 on AECs via inhibiting periostin expression. Overall, our data revealed that GLCCI1 limited the airway remodeling in mice with asthma through inhibiting IL-13/periostin/TGF-ß1 signaling pathway. Our data provided a novel target for asthma treatment.

Hydrothermal combination and geometry control the spatial and temporal rhythm of glacier flow.

The dynamic response of glacier to atmospheric change has varied both spatially and temporally. While some of this variability is likely related to regional climate signals, the geometry of this particular glacier also appears to be important. In this study, we investigated the hydrothermal conditions and geometric controls on the temporal and spatial evolution of Baishui River Glacier No.1's velocity from 2012 to 2019. To do this, we combined field investigations and remote sensing observations to measure the velocity of the glacier, and factors controlling this velocity. Annual changes showed that, from 2012 to 2019, the Baishui River Glacier No. 1 experienced continuous shrinkage, accompanied by decreasing ice velocities. Seasonal changes showed that the glacier velocity during the monsoon period was significantly higher than during the non-monsoon period. Spatially, the glacier's dynamic variability decreased toward its terminus, but increased toward the upper reaches of the glacier, along a longitudinal axis. We would suggest that the interannual velocity variation of Baishui River Glacier No.1 corresponded to thinning of the glacier, which in turn affected its gravitational force. Given that surface melt-induced basal lubrication, basal friction controlled by freezing rate, and dynamic thickening can alter seasonal patterns of movement, these variations may be important for understanding the seasonal evolution of this, and other glaciers. Our results further indicated that glacier width, slope, surface meltwater and crevasses were important constraints on any spatial movement patterns.

Systematic Evaluation of Mycobacterium tuberculosis Proteins for Antigenic Properties Identifies Rv1485 and Rv1705c as Potential Protective Subunit Vaccine Candidates.

The lack of efficacious vaccines against Mycobacterium tuberculosis (MTB) infection is a limiting factor in the prevention and control of tuberculosis (TB), the leading cause of death from an infectious agent. Improvement or replacement of the BCG vaccine with one that reliably protects all age groups is urgent. Concerns exist that antigens currently being evaluated are too homogeneous. To identify new protective antigens, we screened 1,781 proteins from a high-throughput proteome-wide protein purification study for antigenic activity. Forty-nine antigens (34 previously unreported) induced antigen-specific gamma interferon (IFN-γ) release from peripheral blood mononuclear cells (PBMCs) derived from 4,452 TB and suspected TB patients and 167 healthy donors. Three (Rv1485, Rv1705c, and Rv1802) of the 20 antigens evaluated in a BALB/c mouse challenge model showed protective efficacy, reducing lung CFU counts by 66.2%, 75.8%, and 60%, respectively. Evaluation of IgG2a/IgG1 ratios and cytokine release indicated that Rv1485 and Rv1705c induce a protective Th1 immune response. Epitope analysis of PE/PPE protein Rv1705c, the strongest candidate, identified a dominant epitope in its extreme N-terminal domain accounting for 90% of its immune response. Systematic preclinical assessment of antigens Rv1485 and Rv1705c is warranted.