[Effects of Iron Intensity-regulated Root Microbial Community Structure and Function on Cadmium Accumulation in Rice].

Exploring the effects of exogenous iron (Fe) on cadmium (Cd) in rice is of great significance for ensuring food security. The accumulation of Cd and the changes in the microbial community structure in rice roots under three Fe concentrations (5, 50, and 500 µmol·L-1 EDTA-Na2Fe) were studied through a hydroponic experiment. The results showed that the increase in the environmental Fe concentration promoted the formation of iron plaque on the rice roots, and both Fe-deficiency and Fe-sufficiency would enhance the adsorption and fixation of Cd on the root surface. Compared with that of normal Fe levels (50 µmol·L-1), Fe deficiency increased Cd accumulation in rice roots and shoots by 49.76% and 15.68%, respectively. Although Fe sufficiency also increased Cd accumulation in the roots by 18.39%, the Cd concentration in shoots was significantly reduced by 35.95% compared with that of the normal Fe. 16S rRNA high-throughput sequencing was used to determine the root microbial community structure, and through PCA, LEfSe, and RDA analysis, it was found that compared with normal Fe, an Fe-deficient environment reduced the abundance and uniformity of root microbes. Proteobacteria and Bacteroidetes at the phylum level were the dominant flora, Fe deficiency inhibited the increase in the relative abundance of Bacteroidetes, and high-concentration Fe reduced the relative abundance of Proteobacteria. At the genus level, the relative abundance of functional microorganisms Ensifer, Rhodopila, Bdellovibrio, and Dyella were different under different Fe environments, which may have affected the absorption and accumulation of Cd by rice by affecting the formation of Fe plaque on the root and other biochemical processes. In addition, the effect of an Fe-deficient environment on microbial functions was higher than that of the Fe sufficient environment. This study investigated the changes in the rice root microbial community structure and the ability of rice to absorb and transport Cd under different Fe environments, which provided a theoretical basis and an important reference for the inhibition of Fe on Cd accumulation in rice in Cd-polluted paddy soil in southern China.

Influence of straw-derived humic acid-like substance on the availability of Cd/As in paddy soil and their accumulation in rice grain.

Humic acid amendments have been widely advocated for the remediation of heavy metal-contaminated soil. However, the impacts of straw-derived humic acid-like substances on the remediation of cadmium (Cd) and arsenic (As) co-contaminated paddy soil remain unclear and the potential mechanism required clarification. In this study, we employed a pot experiment and chose a straw-derived humic acid-like substance (BFA) as the amendment with four doses to investigate how BFA affects the availability of Cd and As in soil and their accumulation in rice. The results showed that grain Cd decreased by 25.65-36.03%, while there was no significant change in total As (TAs) with the addition of BFA. The contents of DCB-Fe, DCB-As and DCB-Cd on the root surface decreased by 6.07-40.54% during the whole growth stage. The addition of BFA significantly decreased the pe + pH and enhanced the transformation of crystalline iron oxides (Fed) into amorphous forms (Feo) in the soil. The CaCl2-extractable Cd decreased and the KH2PO4-extractable As increased with the decrease in pe + pH and Fed and the relative increase in Feo. The correlation analysis showed that the decrease in availability of Cd and translocation factor of Cd effectively decreased the grain Cd and the decrease in DCB-Cd may also contribute to decreasing the uptake of Cd by rice. However, the increase in As of roots and shoots might play key roles in restricting the transport of As to rice grains. Consequently, the addition of BFA could effectively reduce the Cd accumulation in rice under flooding conditions, while no risk of As accumulation in rice grain was observed. The present work provides a new perspective for the application of straw-derived humic acid-like substances as amendments on Cd-As co-contaminated soils, which should be advocated as an eco-friendly, economical and effective soil amendment in the future.

Sulfur fertilization integrated with soil redox conditions reduces Cd accumulation in rice through microbial induced Cd immobilization.

Sulfate and water management can be respectively applied to control Cd accumulation in rice, but the interaction mechanisms remain unclear. Three water management coupled with five sulfate application concentrations were employed to investigate rice Cd uptake. Results showed there was a significant interaction between sulfate application and soil redox state, and the highest sulfate treatments reduced rice grain Cd by 63.2, 53.5, and 59.4% under the flooding, flooding-moist alternate (FM), and moist irrigation (M) conditions, respectively. It could be explained by the reduction in rhizosphere soil available Cd and lower transport coefficient from root to aboveground. The Desulfovibrio was demonstrated to participate in CdS precipitation, and its abundance was promoted by sulfate especially under flooding. Additionaly, sulfate application facilitated Cd bounded to FeMn oxides, as rhizosphere soil pH raising under flooding. Under FM and M treatments, sulfate application reduced the abundance of Fe-reducing bacteria Geobacter, and correspondingly reduced Fe and Cd availability in rhizosphere soil. Summarily, Cd transfer from soil to rice can be reduced by applying sulfate fertilizer; which is favored by higher soil moisture because of the higher abundance of Desulfovibrio and lower abundance of Geobacter.

Combined exogenous selenium and biochemical fulvic acid reduce Cd accumulation in rice.

Paddy soil Cd contamination and the related accumulation risk in rice grains have attracted global attention. The application of selenium and humic substances is considered to be a cost-effective Cd mitigation measure. However, the effect of a combined application of the two materials remains unclear. Therefore, a 2-season pot experiment was conducted, wherein sodium selenite (Se) and biochemical fulvic acid (BFA) were applied alone and together. Paddy soils with two levels of Cd contamination were used. The results indicate that Se application alone considerably decreased the rice grain Cd content by 36.1-48.7% compared to the control rice grain Cd concentration, which was above the food safety limit (0.2 mg kg-1). Although the application of BFA alone decreased the soil pH, it also increased the soil CaCl2 extractable Cd content by 0.2 to 19.3% and had a limited effect on Cd in the rice grains. The combined application of Se and BFA did not affect the soil pH or the CaCl2 extractable Cd, and more effectively reduced the Cd contents of the rice grains by 50.2 to 57.1%, except for the control rice grain Cd content, which was below the limit. The combined application of Se and BFA also inhibited Se accumulation in rice grains, maintaining the Se content at a safe level (0.33-0.58 mg kg-1) compared to Se application alone. The effects of reducing the Cd content of rice grains while safely increasing their Se contents could persist for at least two seasons. Therefore, the combined application of Se and BFA should be recommended to mitigate Cd contamination risks in Cd-contaminated paddy soil.

Cadmium and arsenic availability in soil under submerged incubation: The influence of humic substances on iron speciation.

Humic substances (HSs), as electron shuttles, are associated with iron oxide transformation, yet the manner by which HSs affect Cd/As availabilities during this process under anaerobic conditions remains unclear. Two HSs (humic sodium, HA-Na, and biochemical fulvic acid, BFA) were applied at 0, 1, 2, and 4 gCkg-1 in a submerged incubation experiment. The dissolved, extractable and fractions of Cd/As and different iron oxides in soils were monitored. The addition of both HA-Na and BFA decreased the CaCl2-extractable Cd by 12.66-93.13%, and increased the KH2PO4-extractable As by 18.81-71.38% on the 60th day of incubation. The soil Eh and crystalline iron oxides (Fed) decreased, while amorphous iron oxides (Feo) and dissolved As increased after addition of both HSs. However, the two HSs had opposite effects on soil pH and dissolved Cd at the end of the incubation. HA-Na immobilized 19.47-85.99% more available Cd than did BFA over the incubation, although the extent of immobilization was similar with the maximum application rate on the 60th day. BFA mobilized 5.22-26.12% more available As than did HA-Na. XPS data showed that FeOOH decreased while the FeO component increased over the incubation. Correlation analysis and SEM showed that the reduction in the soil Eh and Fed and relative increase in Feo increased the available Cd, while decreased the available As. Consequently, the addition of HA-Na and BFA, particularly combined with flooding irrigation management, could effectively reduce the available Cd in Cd-contaminated soil. However, this method should be used with caution in As-contaminated soil.

Meta-analysis of the effects of liming on soil pH and cadmium accumulation in crops.

Increasing cadmium (Cd) contamination in agricultural fields has resulted in a higher risk of Cd accumulation in the food chain. Lime addition can mitigate soil acidification and reduce Cd accumulation in crops cultured in Cd-contaminated soil. To determine key factors controlling the outcomes of liming in reducing Cd accumulation and enhancing soil pH, we performed a meta-analysis using previously published data from field and pot experiments. The results indicated that the liming showed positive effect sizes on the soil pH but negative effect sizes on Cd accumulation in crops, indicating the addition of different lime materials could enhance soil pH and reduce Cd accumulation in crops. The effect sizes of liming on soil pH under pot experimental conditions were higher than that under field experimental conditions, however, the effect sizes of application types and amount of limes on soil pH did not significantly differ between their individual different levels. Under a low background value of soil pH, SOM, CEC and clay, the addition of limes showed a significantly higher effect size on soil pH when compared to their individual higher soil background value, suggesting that the lower background values of soil pH, SOM, CEC and clay might facilitate the outcomes of liming to enhance soil pH. The experiment patterns, crop types and lime application amounts showed a limit effect on the outcomes of liming to reduce the shoot and grain Cd concentrations in crops. The lime types only showed a significant effect size on the shoot Cd accumulation but not on the grain Cd accumulation, in which the CaCO3 had the highest effect size (absolute value, the same below) followed by Ca(OH)2 and CaO. The low soil background values of total Cd concentration and CEC content, but a high soil SOM background content might facilitate the outcomes of liming to reduce the shoot Cd concentration in crops. However, only the background value of soil clay content showed a significantly negative effect size on the grain Cd accumulation, where a high soil clay content had a higher effect size than a low soil clay content. These findings provide useful knowledge about the effects of experiment patterns, crop types, soil conditions, lime types and lime addition amounts on the efficiency of liming in enhancing soil pH and decrease crop Cd concentration.

Influences of different source microplastics with different particle sizes and application rates on soil properties and growth of Chinese cabbage (Brassica chinensis L.).

The potentially negative effects of microplastics (MP) on agroecosystems have raised worldwide concerns. However, little is known about the negative effects of MP exposure on the soil-plant system. To fill up this knowledge gap, a pot experiment was set up, and two different MP types [high density polyethylene (HDPE) and general purpose polystyrene (GPPS)] were used, which had four particle sizes (<25, 25-48, 48-150, and 150-850 µm) at four application rates (2.5, 5, 10, and 20 g MP kg-1 soil). Some soil properties and the growth of Chinese cabbage (Brassica chinensis L.) were monitored. The results showed that (1) MP application with high application rates and relatively small particle sizes significantly enhanced the soil urease activity, which accompanied with enhanced soil pH and decreased soil available concentrations of phosphorus and potassium in some cases. (2) The exposure of MP did not significantly affect the activity of soil catalase regardless of their application rates and sizes. MP with different application rates and small sizes significantly reduced the soil sucrase activity, but the largest size of MP enhanced the activity of soil sucrase. (3) GPPS at 10-20 g kg-1 or with the sizes of <25 and 48-150 µm significantly reduced the fresh weight of Chinese cabbage, but the addition of HDPE had no remarkable effects on the fresh weight regarding of its application rates or sizes. (4) MP with high application rates and large sizes enhanced but small sizes of MP reduced the leaf soluble sugar concentration. The increasing application rates of MP and small size HDPE significantly reduced the starch concentration in the leaves of Chinese cabbage, however, the different sizes of GPPS showed limited effects on the leaf starch. The addition of MP with increasing application rates and different sizes always reduced the concentration of leaf chlorophyll. These parameters regarding to plant and soil could be used to assess the risks of MP pollution in the soil-plant systems. We found that the risks resulting from MP pollution were MP type-dependent and particle size-dependent. These findings indicate that overaccumulation of MP in the agriculture may possess an ecology risk and will negatively affect the agricultural sustainability and the food safety.

[Effects of Water Management on Cadmium Accumulation by Rice (Oryza sativa L.) Growing in Typical Paddy Soil].

In order to explore the effects of water management on the Cd accumulation of rice in paddy soils with different parent materials, a pot experiment with three paddy soils with different parent materials from Hunan Province (granite sandy soil, plate shale soil, and purple sandy shale soil) with different water management treatments ï¼»flooding and alternate wetting and drying (AWD)ï¼½ was performed. The soil pH, DTPA-Cd, Fe plaque in the rice roots, and heavy metal concentration in the rice were determined. The results showed that the soil pH of the three paddy soils under the flooding treatment was increased by 0.17-1.33 units. During the filling and maturity periods, compared with that under AWD, the DTPA-Cd concentration in the three paddy soils was reduced by 14.39%-36.56% under the flooding treatment, but the DTPA-Fe concentration was increased by 35.35%-347.25%. In the three growth stages, the Cd and Mn concentrations in the Fe plaque (except for DCB-Fe) were in the order of tillering stage < filling stage < mature stage. Compared with that under AWD, the brown rice Cd concentration in the three soils was reduced by 57.84%-93.79% under flooding treatment. The Cd accumulation in rice was reduced under flooding treatment by reducing the DTPA-Cd via increasing the soil pH and DTPA-Fe and by decreasing the formation of Fe plaque. According to the results of the correlation and SEM analysis, the soil pH and DCB-Cd were the main factors affecting the Cd accumulation in rice grains, although the changes in the DTPA-Cd and DTPA-Fe also impacted the Cd in rice grains. In summary, our study demonstrated that water management had a significant impact on the Cd content in rice, and there were significant differences among the three paddy soils with different parent materials. In conclusion, the Cd content in rice grains was affected by the soil parent material, soil physicochemical properties, and Fe plaque on the surface of the rice roots. The granite sandy soil and plate shale soil with different water management treatments had significant impacts on the contents of heavy metals in rice. Continuous flooding is a valuable strategy for improving soil acidity and alkalinity and minimizing soil available Cd, but the soil parent materials must be considered.

Agronomic traits and ionomics influence on Cd accumulation in various sorghum (Sorghum bicolor (L.) Moench) genotypes.

Cd is a common pollutant that contaminates the ecological environment of soil-crop systems and threatens food security and human health. Sorghum (Sorghum bicolor (L.) Moench) has a great potential for use as energy feedstock and Cd phytoremediation. Therefore, the identification of sorghum genotypes with high Cd accumulation is of great significance to Cd pollution remediation and production of bioenergy. A total of 126 biomass sorghum genotypes grown in a Cd-polluted field were investigated, and their agronomic traits were analyzed, including plant height, leaf number, shoot dry weight (SDW), soil and plant analyzer development (SPAD) value, and concentration of metal ions at seedling stage. Plant height was an important factor for screening potential biomass sorghum species because it presented a significant correlation with the Cd concentration in shoots and SDW (P < 0.01). The highest and lowest Cd concentration in sorghum shoots were 7.88 and 0.99 mg kg-1, respectively. The Cd concentration presented a negative and significant correlation with Mn in sorghum shoots (r = -0.303, P < 0.01), which was in agreement with the results that sorghum species with high Cd concentrations have lower Mn concentrations. In the mature stage, sorghum 12530 presented higher Cd concentration and dry weight in shoots compared with other genotypes. In summary, plant height, SDW, and concentration of Mn in sorghum shoots are critical parameters that synthetically influence the accumulation of Cd in sorghum shoots.

Effects of composited organic mobilizing agents and their application periods on cadmium absorption of Sorghum bicolor L. in a Cd-contaminated soil.

Organic mobilizing agents have been advocated for phytoremediation of heavy metals contaminated soils, while the effects of application period of such agents remain unclear. A pot experiment was conducted, with two composited organic agents (oxalic acid or citric acid + dissolved organic fertilizer (OA + DOF and CA + DOF)) and four application periods (seeding, jointing, flag leaf and heading stages) of sorghum (Sorghum bicolor L.), to investigate their impacts on Cd bioavailability in soil. Results indicated that application of the two composited agents increased soil dissolved organic carbon (DOC) and DTPA extractable Cd by 7.31-49.13%, Cd contents in roots and shoots by 21.49-72.10%, bioaccumulation factor (BCF) and translocation factor (TF) of shoots by 4.44-71.99%, while reduced soil pH by 0.25-0.53 units, respectively. Most of these indices increased with the application periods, and largely peaked with their application during the flag leaf to heading stages. Meanwhile, the maximum sorghum biomass (132.84 g pot-1) and Cd bioaccumulation quantity (BCQ, 0.71 mg pot-1) in shoots were obtained for the CA + DOF applied at the heading. The DTPA extractable Cd was closely related to soil pH and DOC. Similar close relationships were observed between the Cd contents in shoots and soil DTPA extractable Cd, pH and DOC. The BCQ of Cd was positively related to the shoots biomass rather than their Cd contents. Therefore, the sorghum combined with the CA + DOF may be advocated as an alternative phytoremediation mode in Cd-contaminated soils, and the mobilizing agent should be primarily applied at the heading stage.

A Novel Method with Stacking Learning of Data-Driven Soft Sensors for Mud Concentration in a Cutter Suction Dredger.

The dredger construction environment is harsh, and the mud concentration meter can be damaged from time to time. To ensure that the dredger can continue construction operations when the mud concentration meter is damaged, the development of a dredger with advantages of low price and simple operation that can be used in emergency situations is essential. The characteristic spare mud concentration meter is particularly critical. In this study, a data-driven soft sensor method is proposed that can predict the mud concentration in real time and can mitigate current marine mud concentration meter malfunctions, which affects continuous construction. This sensor can also replace the mud concentration meter when the construction is stable, thereby extending its service life. The method is applied to two actual construction cases, and the results show that the stacking generalization (SG) model has a good prediction effect in the two cases, and its goodness of fit R2 values are as high as 0.9774 and 0.9919, indicating that this method can successfully detect the mud concentration.

Increasing soil moisture faciliates the outcomes of exogenous sulfate rather than element sulfur in reducing cadmium accumulation in rice (Oryza sativa L.).

Cadmium (Cd) contamination in paddy soils and the related pollution risk of rice grain have received increasing attention. Agronomic measures, such as the application of sulfur and changes in water regimes, were reported to mitigate the accumulation of Cd in rice. However, there is limited information on the combined effects of sulfur application and water regimes. Therefore, a pot experiment was conducted to investigate the effects of two sulfur forms, three water regimes and multiple sulfur application rates on Cd accumulation in rice. The sulfur was applied as SO42- (SVI, replacing the traditional fertilizers by SO42--containing fertilizers), and element S (S0) was applied at 0, 10, 20, 30 and 40 mg S kg-1 soil. The water regimes were continuous flooding (F), flooding-moist alternation (FM), and moist irrigation (M), for a total of 30 treatments. The results indicated that application of SVI exceeding 30 mg S kg-1 significantly reduced the Cd concentrations in brown rice by 31.1-56.3%, and the Cd concentrations decreased with increasing amount of irrigation water. Similar reductions in Cd concentrations in rice shoots and rice straw collected at tillering and maturity stages were observed after application of SVI. However, the effect of S0 application on Cd accumulation in grain was not significant under different water regimes. Furthermore, this study found that application of both SVI and S0 inhibited the transfer of Cd from rice roots to shoots in most cases. These findings indicate that replacing traditional fertilizers with SO42--containing fertilizers, especially combined with increased irrigation, could be a potential approach to mitigate Cd accumulation in rice growing in Cd-contaminated acidic paddy soils.

MicroRNA-486-5p targeting PTEN Protects Against Coronary Microembolization-Induced Cardiomyocyte Apoptosis in Rats by activating the PI3K/AKT pathway.

Coronary microembolization (CME) is responsible for a substantial fraction of microvascular obstruction (MVO), which are strongly associated with mortality and hospitalization for heart failure within 1 year after primary percutaneous coronary intervention (PCI) in ST-segment elevation myocardial infarction (STEMI). However, the effect of miRNA on cardiomyocyte apoptosis in a CME model has been less well-studied. miRNA sequencing analysis was performed to examine differentially expressed miRNAs induced by CME in rats. Phosphatase and tensin homologue (PTEN) 3 'RACE and dual-luciferase reporter assays were performed to confirm that PTEN is a direct target gene of miR-486-5p. miRNA-486-5p overexpression was established by injecting AAV into rats via the tail vein. The CME model was established by injecting microspheres into the left ventricle of rats. 6h after surgery, cardiac function, microinfarct area, and the apoptotic index were determined. RT-PCR was used to evaluate mRNA level and Western blotting was used to evaluate protein expression. miRNA sequencing data showed that there were 5 upregulated and 8 downregulated miRNAs, and the relative expression of miRNA-486-5p was significantly downregulated. PTEN 3'RACE and dual-luciferase reporter assays confirmed that miR-486-5p directly targets the rat PTEN gene. The expression of miR-486-5p gradually declined, however, the expression of PTEN mRNA rapidly increased at early time points after CME. Overexpression of miR-486-5p reduced cardiomyocyte apoptosis and improved cardiac function through inhibition of PTEN and activation of the PI3K/Akt pathway in rat CME models. Overexpression of miR-486-5p, which targets PTEN, protects against CME-induced cardiomyocyte apoptosis and improves cardiac function in rats by activating the PI3K/Akt pathway.

Egr-1 is involved in coronary microembolization-induced myocardial injury via Bim/Beclin-1 pathway-mediated autophagy inhibition and apoptosis activation.

Coronary microembolization (CME) substantially reduces the clinical benefits of myocardial reperfusion therapy. Autophagy and apoptosis participate in the pathophysiological processes of almost all cardiovascular diseases, including CME-induced myocardial injury, but the precise underlying mechanisms remain unclear. In the present study, we observed that Egr-1 expression was substantially increased after CME modeling. Inhibition of Egr-1 expression through the targeted delivery of rAAV9-Egr-1-shRNA improved cardiac function and reduced myocardial injury. The microinfarct size was also significantly smaller in the Egr-1 inhibitor group than in the CME group. These benefits were partially reversed by the autophagy inhibitor 3-MA. As shown in our previous study, autophagy in the myocardium was impaired after CME. Inhibition of Egr-1 expression in vivo restored the autophagy flux and reduced myocardial apoptosis, at least partially, by inhibiting the Egr-1/Bim/Beclin-1 pathway, as evidenced by the results of the western blot, RT-qPCR, and TUNEL staining. At the same time, TEM showed a dramatic increase in the number of typical autophagic vacuoles in the Egr-1 inhibitor group compared to the CME group. Based on these findings, the Egr-1/Bim/Beclin-1 pathway may be involved in CME-induced myocardial injury by regulating myocardial autophagy and apoptosis, and this pathway represents a potential therapeutic target in CME.

Effectiveness of simultaneous applications of lime and zinc/iron foliar sprays to minimize cadmium accumulation in rice.

Due to the large area of Cd-contaminated paddy soils worldwide, low-cost measures to reduce the accumulation of Cd in rice plant are necessary. A field experiment was therefore conducted to investigate the reducing effect of lime combined with foliar applications of Zn (ZnSO4) or Fe (EDTA·Na2Fe) on Cd concentrations in brown rice on a Cd-contaminated paddy soil. The results indicated that liming alone or in combination with foliar sprays of Zn or Fe increased the soil pH by 0.27-0.63 units. However, limited effects of lime or lime combined with foliar applications of Zn/Fe on soil DTPA-extractable Cd, rice grain and rice straw biomass were observed. Liming alone significantly reduced the Cd concentration in brown rice and rice straw by 31.8% and 42.3%, respectively. The Cd concentrations in brown rice decreased by 25.5% and 65.4% and in rice straw by 53.0% and 68.1% after liming combined with foliar applications of Fe and Zn, respectively. In contrast, liming combined with foliar spraying of Fe significantly increased the transfer ratio of Cd from the rice straw to the grain. As a low-cost technique, lime application combined with foliar application of ZnSO4 could be recommended for the remediation of Cd-contaminated paddy soils.

Pim1 Overexpression Prevents Apoptosis in Cardiomyocytes After Exposure to Hypoxia and Oxidative Stress via Upregulating Cell Autophagy.

BACKGROUND/AIMS: Microvascular obstruction (MVO), an undesirable complication of percutaneous coronary intervention, is independently associated with adverse left ventricle remodeling and poor prognosis after acute myocardial infarction. Hypoxia and oxidative stress major roles in the pathophysiology of MVO. Pim1 serves an important protective role in the ischemic myocardium, but the underlying mechanisms remain poorly defined. Autophagy in early hypoxia or during moderate oxidative stress has been demonstrated to protect the myocardium. In this study, we investigated the association between the protective effect of Pim1 and autophagy after hypoxia and oxidative stress. METHODS: Ventricular myocytes from neonatal rat heart (NRVMs) were isolated. NRVMs were exposed to hypoxia and H2O2. Rapamycin and 3-methyladenine (3-MA) were used as an activator and inhibitor of autophagy, respectively. pHBAd-Pim1 was transfected into NRVMs. We assessed cardiomyocyte apoptosis by Annexin V-FITC/PI flow cytometry. Autophagy was evaluated by mRFP-GFP-LC3 adenovirus infection by confocal microscopy. Western blotting was used to quantify apoptosis or autophagy protein (caspase-3, LC3, P62, AMPK, mTOR, ATG5) concentrations. RESULTS: Autophagy and apoptosis in NRVMs significantly increased and peaked at 3 h and 6 h, respectively, after exposure to hypoxia and H2O2. The mTOR inhibitor rapamycin induced autophagy and decreased cardiomyocyte apoptosis, but the autophagy inhibitor 3-MA decreased autophagy and increased apoptosis at 3 h after exposure to hypoxia and H2O2. Pim1 levels in NRVMs increased at 3 h and decreased gradually after exposure to hypoxia and H2O2. Pim1 overexpression enhanced autophagy and decreased apoptosis. Pim1-induced promotion of autophagy is partly the result of activation of the AMPK/mTOR/ATG5 pathway after exposure to hypoxia and H2O2. CONCLUSION: Our results revealed that Pim1 overexpression prevented NRVMs from apoptosis via upregulating autophagy after exposure to hypoxia and oxidative stress, partly through activation of the AMPK/mTOR/ATG5 autophagy pathway.

Foliar application of Zn can reduce Cd concentrations in rice (Oryza sativa L.) under field conditions.

Cadmium (Cd) pollution in rice and its transfer to food chain are cause of global concern. Application of zinc (Zn) can reduce Cd uptake by plants, as both these metals are generally antagonistic in soil-plant systems. In a field experiment on Cd-contaminated acid soil, we investigated the effectiveness of foliar application of Zn in minimizing Cd accumulation and its effect on the content of mineral nutrient elements in rice. The treatment was done at an early grain filling stag using 0.3 and 0.5% w/v ZnSO4·7H2O solution. The spray did not affect the grain yield of rice but decreased the Cd concentration in the root, straw, husk, and brown rice to some extent and increased the Zn concentration. Foliar application of 0.5% ZnSO4 resulted in maximum Zn concentration and minimum Cd concentration in brown rice. However, the concentrations of P, K, Ca, Mg, Cu, and Mn in brown rice were not affected. The correlation between Cd and Zn concentrations in brown rice, husk, and root was significantly negative, and that between Cd and Mn concentrations in brown rice was significantly positive. The inhibition of Cd uptake resulted in a decrease in its concentration in brown rice after the treatments. Thus, the foliar application of a suitable concentration of Zn at the early grain filling stage could effectively minimize the Cd concentration while enhancing the Zn concentration in brown rice on Cd-contaminated acid soil.

Distribution and availability of cadmium in profile and aggregates of a paddy soil with 30-year fertilization and its impact on Cd accumulation in rice plant.

The research was conducted to investigate the accumulation, distribution and availability of Cd in paddy soil and their relation to Cd in rice plants under 30-year fertilization regimes. Six treatments were involved in the study: control without fertilization (CK), chemical fertilizer (NPK), high nitrogen chemical fertilizer (HN), rice straw incorporation (ST), low and high dosage of manure fertilizer (LM and HM). Total and DTPA extractable concentration of Cd (T-Cd and DTPA-Cd) in bulk soils (20 cm topsoil), profiles (0-60 cm) and aggregates (>2, 1-2, 0.5-1, 0.25-0.5, 0.053-0.25 and < 0.053 mm) were investigated. The Cd concentration in relevant rice plant (roots, stems, leaves, husks and grains) were also analyzed. Manure fertilizers caused T-Cd accumulation in bulk soil with a significant increase of 36.2% in LM and 81.2% in HM. Similar impacts of manure fertilizers were observed in DTPA-Cd in the bulk soil. Further, the HM generated a further accumulation in deeper soil layers, presenting a remarkable increase of T-Cd (28.3%-225%) in 10-40 cm and DTPA-Cd (116%-158%) in 10-30 cm profiles. Moreover, the continuous application of manure fertilizers enhanced the availability of Cd in all aggregate size classes with an increase of 17.3%-87.8% in DTPA-Cd. Organic fertilizers (LM, HM and ST) heightened the content of Cd (38.0%-152%) in all parts of rice plant. The accumulation of Cd in rice plants was directly affected by fertilization regimes and Cd availability in the 10-20 cm soil layers and 0.25-0.5 mm aggregates. In conclusion, long-term application of manures resulted in increasing availability of Cd in aggregates and in topsoil and subsoil layers, which accordingly enhanced the accumulation of Cd in rice plants.

Effect of peanut shell and wheat straw biochar on the availability of Cd and Pb in a soil-rice (Oryza sativa L.) system.

Soil amendments, such as biochar, have been used to enhance the immobilization of heavy metals in contaminated soil. A pot experiment was conducted to immobilize the available cadmium (Cd) and lead (Pb) in soil using peanut shell biochar (PBC) and wheat straw biochar (WBC), and to observe the accumulation of these heavy metals in rice (Oryza sativa L.). The application of PBC and WBC led to significantly higher pH, soil organic carbon (SOC), and cation exchange capacity (CEC) in paddy soil, while the content of MgCl2-extractable Cd and Pb was lower than that of untreated soil. MgCl2-extractable Cd and Pb showed significant negative correlations with pH, SOC, and CEC (p < 0.01). The application of 5% biochar to contaminated paddy soil led to reductions of 40.4-45.7 and 68.6-79.0%, respectively, in the content of MgCl2-extractable Cd and Pb. PBC more effectively immobilized Cd and Pb than WBC. Sequential chemical extractions revealed that biochar induced the transformation of the acid-soluble fraction of Cd to oxidizable and residual fractions, and the acid-soluble fraction of Pb to reducible and residual fractions. PBC and WBC clearly inhibited the uptake and accumulation of Cd and Pb in rice plants. Specially, when compared to the corresponding concentrations in rice grown in control soils, 5% PBC addition lowered Cd and Pb concentrations in grains by 22.9 and 12.2%, respectively, while WBC addition lowered them by 29.1 and 15.0%, respectively. Compared to Pb content, Cd content was reduced to a greater extent in grain by PBC and WBC. These results suggest that biochar application is effective for immobilizing Cd and Pb in contaminated paddy soil, and reduces their bioavailability in rice. Biochar could be used as a soil amendment for the remediation of soils contaminated with heavy metals.

Potential Involvement of MiR-30e-3p in Myocardial Injury Induced by Coronary Microembolization via Autophagy Activation.

BACKGROUND/AIMS: Coronary microembolization (CME) can lead to no-reflow or slow reflow, which is one of the important reasons for loss of clinical benefit from myocardial reperfusion therapy. MicroRNAs and autophagy are heavily implicated in the occurrence and development of almost all cardiovascular diseases. Therefore, the present study was designed to investigate the role of miR-30e-3p and autophagy in CME-induced myocardial injury rat model. METHODS: Sixty rats were randomly divided into six groups: sham, CME 1h,3h,6h,9h, and 12h (n = 10 per group). Our CME rat model was created by injecting polyethylene microspheres (42mm) into the left ventricle of the heart; the sham group was injected with same volume of normal saline. The cardiac function and serum cardiac troponin I (cTnI) level of each group was measured. HE staining and HBFP staining were used to evaluate the myocardial micro-infarction area of myocardium tissue samples. Then RT-qPCR and western blot were used to detect the expression of miR-30e-3p and, autophagy related protein LC3-II and p62, respectively. Transmission electron microscope (TEM) was used to identify autophagic vacuoles in tissue samples. RESULTS: The cardiac function of the CME 6h,9h, and 12h groups were significantly decreased compared to the sham group (P < 0.05) and the cTnI level in each group were also significantly increased (P < 0.05). The expression of miR-30e-3p in the CME 6h, 9h and 12h group were decreased significantly compared with the sham group (P < 0.05). Meanwhile, the expression of autophagy related protein LC3-II decreased significantly and p62 increased significantly in the CME 9h and 12h group (P < 0.05). TEM images showed typical autophagic vacuoles for each of the CME groups. CONCLUSIONS: Myocardial miR-30e-3p is down regulated after CME and is accompanied by inhibited autophagy and decreased cardiac function. Therefore, miR-30e-3p may be involved in CME-induced cardiac dysfunction by regulating myocardial autophagy.