Submersed macrophytes Vallisneria natans and Vallisneria spinulosa improve water quality and affect microbial communities in sediment and water columns.

Healthy aquatic ecosystems are essential for human beings. However, anthropogenic activities severely worsen water quality. In this study, using assembling mesocosms, we developed an efficient and easy-to-handle method to monitor the water quality by measuring the electrical conductivity (EC) of water. Our data demonstrate that the growth of two submersed macrophytes, Vallisnerianatans and Vallisneria spinulosa, improves water quality by decreasing EC. Furthermore, using high-throughput DNA sequencing, we analyzed the microbial community abundance and structure in sediment and water columns with or without plant growth. We generated 33,775 amplicon sequence variants from 69 samples of four sediment groups (BkM, CtM, VnR, and VsR) and three water column sample groups (CtW, VnW, and VsW). The results show that the relative abundance of bacteria was higher in the sediment than in the water column. Moreover, the diversity and composition of microbiomes were altered by Vallisneria spp. growth, and the α-diversity of the microbial communities decreased due to submersed macrophytes in both the sediment and water columns. The ß-diversity of the microbial communities also varied significantly with or without Vallisneria spp. growth for both the sediment and water columns.

[Regulatory Mechanism of Mangiferin Combined with Bortezomib on Malignant Biological Behavior of Burkitt Lymphoma and Its Effect on Expression of CXC Chemokine Receptors].

OBJECTIVE: To analyze the effects of mangiferin combined with bortezomib on the proliferation, invasion, apoptosis and autophagy of human Burkitt lymphoma Raji cells, as well as the expression of CXC chemokine receptors (CXCRs) family, and explore the molecular mechanism between them to provide scientific basis for basic research and clinical work of Burkitt lymphoma. METHODS: Raji cells were intervened with different concentrations of mangiferin and bortezomib alone or in combination, then cell proliferation was detected by CCK-8 assay, cell invasion ability was detected by Transwell chamber method, cell apoptosis was detected by Annexin V/PI double-staining flow cytometry, apoptosis, autophagy and Akt/mTOR pathway protein expression were detected by Western blot, and the expression changes of CXCR family was detected by real-time quantitative PCR (RT-qPCR). RESULTS: Different concentrations of mangiferin intervened Raji cells for different time could inhibit cell viability in a concentration- and time-dependent manner (r =-0.682, r =-0.836). When Raji cells were intervened by combination of mangiferin and bortezomib, compared with single drug group, the proliferation and invasion abilities were significantly decreased, while the apoptosis level was significantly increased (P <0.01). Mangiferin combined with bortezomib could significantly up-regulate the expression of pro-apoptotic protein Bax and down-regulate the expression of anti-apoptotic protein Bcl-2 after intervention in Raji cells. Caspase-3 was also hydrolyzed and activated, and then induced the apoptosis of Raji cells. Mangiferin combined with bortezomib could up-regulate the expression of LC3Ⅱ protein in Raji cells, and the ratio of LC3Ⅱ/LC3Ⅰ in cells was significantly up-regulated compared with single drug or control group (P <0.01). Mangiferin combined with bortezomib could significantly inhibit the phosphorylation levels of Akt and mTOR, inhibit the proliferation and invasion of Raji cells by inhibiting Akt/mTOR pathway, and induce cell autophagy and apoptosis. Mangiferin and bortezomib could down-regulate the expressions of CXCR4 and CXCR7 mRNA after single-agent intervention in Raji cells, and the down-regulations of CXCR4 and CXCR7 mRNA expression were more significant when the two drugs were combined (P <0.01). Mangiferin alone or combined with bortezomib had no significant effect on CXCR5 mRNA expression in Raji cells (P >0.05), while the combination of the two drugs could down-regulate the expression of CXCR3 (P <0.05). CONCLUSION: Mangiferin combined with bortezomib can synergistically inhibit the proliferation and invasion of Raji cells, and induce autophagy and apoptosis. The mechanism may be related to the inhibition of Akt/mTOR signaling pathway, down-regulation of anti-apoptotic protein Bcl-2 and up-regulation of pro-apoptotic protein Bax, and the inhibition of the expression of CXCR family.

7-Ketocholesterol accelerates pancreatic ß-cell senescence by inhibiting the SIRT1/CDK4-Rb-E2F1 signaling pathway.

Pancreatic ß-cell dysfunction is a key factor in the development of type 2 diabetes. Pancreatic ß-cell senescence accelerates abnormal glucose metabolism, which decreases insulin secretion and cell regeneration ability, eventually leading to diabetes. A cholesterol oxidation product, 7-ketocholesterol (7-KC) can affect pancreatic ß-cell function. However, its role in pancreatic ß-cell senescence has not been reported. We investigated the role of 7-KC in pancreatic ß-cell senescence and its underlying molecular mechanism in MIN6 cells. MIN6 cells were treated with 25 µmol/L 7-KC for 24 h and the proportion of senescent cells was detected based on senescence-associated ß-galactosidase (SA-ß-gal) activity. The cell cycle, DNA damage, and the senescence-associate secretory phenotype (SASP) and protein expression were detected by flow cytometry, immunofluorescence, and western blotting, respectively. 7-KC can significantly increase SA-ß-gal activity, promoted G0/G1 arrest, DNA damage, and interleukin-1ß expression in MIN6 cells and significantly inhibited insulin synthesis. Further studies indicated that 7-KC induced ß-cell senescence by inhibiting the SIRT1/CDK4-Rb - E2F1 signaling pathway.

Case Report: Recurrent Autoimmune Hypoglycemia Induced by Non-Hypoglycemic Medications.

We present a case of recurrent autoimmune hypoglycemia induced by non-hypoglycemic agents. We review reported cases of autoimmune hypoglycemia related to non-hypoglycemic agents, and discuss the effects of different detection methods for insulin autoantibodies on the results obtained. We aim to provide information for clinicians and a warning for medication usage. Considering the increasing number of clopidogrel-induced AIH cases and the hypoglycemia-induced increase in the risk of cardiovascular events, we recommend that cardiovascular disease patients being treated with clopidogrel be informed of this rare side effect and that clinicians be vigilant for the possibility of autoimmune hypoglycemia in this patient population.

Hydrological isolation affected the chemo-diversity of dissolved organic matter in a large river-connected lake (Poyang Lake, China).

The transportation processes during aquatic systems regulate the ultimate chemistry of dissolved organic matter (DOM), and in recent years, climate changes and human activities have altered the hydrological patterns of many rivers and lakes, which generated some severe issues, such as hydrological isolation. However, how hydrological isolation affects variations of DOM chemistry in large lake systems is still poorly understood. Here, optical properties and molecular compositions of DOM samples derived from a large river-connected lake (Poyang Lake, China) and its nearby seasonal sub-lakes (formed by hydrological isolation) were characterized using Fourier transform ion cyclotron resonance mass spectrometry (FT ICR MS) and ultraviolet-visible (UV-Vis) spectroscopy. The results revealed more abundance of organic matter in sub-lakes than that in the main lake according to high dissolved organic carbon (DOC) concentrations and absorption coefficients (a254 and a280). Large proportions of CHOS formulas were identified by FT ICR MS in sub-lakes DOM, which were produced through Kraft reactions (sulfide/bisulfide + lignin CHO → CHOS) in the interface of sediment/water, and greatly contributed to aliphatic compounds. In addition, obvious variations of compounds (such as polyphenols, highly unsaturated and aliphatic compounds) and lability of DOM were observed between sub-lakes and main lakes, which were mainly caused by the different degradation pathways of DOM (photodegradation in sub-lakes while biodegradation in the main lake). Our results demonstrated that hydrological isolation has significant impacts on DOM chemistry, and provides an improved understanding of the DOM biogeochemistry process in Poyang Lake and supports the management of the large lake systems.

The regulatory effect of herd structure on pig production under the environmental regulation.

Integrating pig production stability and pollution control is a matter of social livelihood and green development. This paper estimates the policy effectiveness of environmental regulation based on the big data of Chinese government websites and combines the pig production data of various provinces in China from 2008 to 2018 to construct a mediating effect model, empirically analyzing the relationship between environmental regulations, herd structure, and pig production. Research shows: (1) Improving environmental regulations in the short term will increase the slaughter of pigs, but in the long run it will decrease first and then increase; (2) At this stage, environmental regulations can increase the slaughter of pigs by changing the herd structure; (3) Reasonable adjustment of the herd structure can effectively increase the slaughter of pigs; (4) The herd structure can be used as a supplementary monitoring indicator to stabilize the supply of pigs. Combine it with the change rate of sow stock to provide early warning of fluctuations in pig production, and the early warning herd structure values are 0.0980 and 0.1135. There are two key initiatives to achieve supply and price stability in the pig industry that taking into account environmental protection: first, an industrial regulation instrument based on the herd structure needs to be established; second, an early warning system for pig production fluctuations should be established with reference to the early warning system for pork price fluctuations.

Expression of serum autophagy-related protein P62 in patients with severe pancreatitis and its correlation with prognosis.

OBJECTIVE: This study was designed to investigate the expression of serum autophagy-related protein P62 in patients with severe acute pancreatitis (AP) and its correlation with prognosis. METHODS: Eighty patients with AP treated in the First Affiliated Hospital of Gannan Medical University from January 2020 to January 2021 were enrolled as study subjects in this retrospective analysis, and they were placed into the mild AP group (n=52) or the severe AP group (n=28). According to clinical outcomes, these 80 patients were divided into a good prognosis group (GP group, n=51, surviving without serious complications such as organ failure) and a poor prognosis group (PP group, n=29, death or developing organ failure). The differences in C-reactive protein (CRP), P62 and the Acute Physiology and Chronic Health Evaluation II (APACHE-II) were compared upon admission. The changes of CRP, P62 and APACHE-II within 1-7 h after admission were dynamically analyzed in the two groups. Spearman correlation analysis was performed to explore the correlation between P62 and APACHE-II scores, and the receiver operating characteristic (ROC) curve of P62 related to poor AP outcome was plotted. RESULTS: CRP, P62 and APACHE-II in the mild AP group were significantly higher than those in the severe AP group, and these in the PP group were also significantly higher than those in the GP group (P<0.05). Dynamic monitoring showed that within 1-7 h after admission, CRP, P62, and APACHE-II in the severe AP group were significantly higher than those in the mild AP group (P<0.05), and these in the PP group were significantly higher than those in the GP group (P<0.05). Spearman correlation analysis showed that P62 level was significantly positively correlated with both CRP and APACHE-II (r=0.9331, r=0.9500, P<0.0001). ROC curve showed that AUC of P62 was 0.9570 in AP patients with poor prognosis (95% CI=0.8939-1.000, P<0.0001). CONCLUSION: Serum autophagy-related protein P62 was closely related to the condition and prognosis of AP patients, and P62 could be used as a potential indicator to assess the condition and prognosis of AP patients.

Coexistence of thrombotic thrombocytopenic purpura and immune thrombocytopenic purpura in an Asian woman: a case report.

A 33-year-old Chinese woman with a history of immune thrombocytopenic purpura presented with heavy menstrual bleeding. She was found to have thrombocytopenia, plasma ADAMTS13 activity of 0%, and positivity for the plasma ADAMTS13 inhibitor. She was diagnosed with the coexistence of thrombotic thrombocytopenic purpura and immune thrombocytopenic purpura. The patient was treated by plasmapheresis, a glucocorticoid, and rituximab. Her platelet level returned to normal, and she was discharged 28 days after admission. The number of plasmapheresis sessions and the timing of rituximab administration may be the key aspects of management of patients with thrombotic thrombocytopenic purpura who have underlying immune dysfunction caused by diseases such as immune thrombocytopenic purpura.

The payment scheme for ecosystem services in the coastal city based on the ecosystem services value and current payment efficiency: a case study in Jimo of Qingdao city, China.

The reasonable strategies of payment for ecosystem services (PES) play a key role in solving the contradiction between ecological protection and economic development in coastal cities. However, at present, the payment efficiency is relatively low and the determination method of PES is lack. Therefore, in this study, the Jimo district of Qingdao city in China, a typical coastal city, was selected as study area, and the equivalent factor method and marine ecological capital assessment method were used to obtain terrestrial and marine ecosystem services value (ESV) and its tempo-spatial variations from 2010 to 2018. Moreover, the payment efficiency of the sea area and 15 towns over 8 years was measured using the Super-efficient SBM-DEA model based on undesired output. ESV presented a clear reduction over 8 years. The marine ecosystem provided the main service value, and waste treatment, water regulation, and soil formation were the top 3 main functions in the land ecosystem. Regulating services provided the highest component among all functions. The high-value areas were mainly distributed in the northeast and northwest regions and the low-value areas were in the south-central regions. The average payment efficiency of the sea area and 15 towns over 8 years shown a stable trend at a low level. On the basis of the evaluation of ESV and efficiency, a new possible payment scheme including payment order and quota was proposed. The total quota of marine ecosystem was 5.226 billion RMB (88% of the total) and of terrestrial ecosystem was 0.713 billion RMB. Tian Heng, Jin Kou, Yi Fengdian, and Ling Shan are the first 4 priority towns, with compensation amounts of 0.205, 0.083, and 0.063 billion RMB respectively, while the last 3 towns are Tong Ji, Huan Xiu, and Chao Hai accounting for 6.02% of the total. The theoretical payment total quota was 5.939 billion RMB, and which accounted for a low proportion of local GDP. The study can provide some recommendations for making the reasonable and feasible payment schemes for ecosystem services in coastal city, and it is feasible in the practice of ecological environment protection and sustainable development.

The predictive value of lung ultrasound combined with central venous oxygen saturation variations in the outcome of ventilator weaning in patients after thoracic surgery.

OBJECTIVE: To evaluate the predictive value of lung ultrasound score (LUS) combined with central venous oxygen saturation variations (ΔScvO2) in the outcome of ventilator weaning in patients after thoracic surgery. METHODS: The clinical data of 60 patients who received tracheal intubation ventilator-assisted breathing after thoracic surgery were retrospectively analyzed, and they were divided into successful (n = 35) and failed (n = 25) groups according to the postoperative weaning outcomes. The factors influencing the failure of weaning in patients after thoracic surgery were compared and analyzed, and the values of LUS, ΔScvO2 as well as the combination of both were calculated to predict the failure of weaning in patients after thoracic surgery. RESULTS: The results of logistic regression analysis showed that LUS, ΔScvO2, and partial pressure of carbon dioxide (PaCO2) may be risk factors influencing weaning failure in patients after thoracic surgery (OR = 1.844, 4.006, 1.271, P < 0.001 for all), while diaphragm thickening fraction (DTF) and partial pressure of oxygen (PaO2) may be protective factors (OR = 0.852, 0.674, P = 0.002 for all). Receiver operator characteristic (ROC) curve showed that area under the curves (AUCs) of LUS, ΔScvO2, and the combination of the two was 0.865 (95% CI: 0.766-0.964), 0.874 (95% CI: 0.781-0.967), and 0.925 (95% CI: 0.860-0.990), respectively, in predicting failure of weaning in patients after thoracic surgery. CONCLUSION: LUS and ΔScvO2 were closely related to chest ultrasound index and arterial blood gas index in patients after thoracic surgery, both of which may be risk factors for weaning failure in patients after thoracic surgery, and their combination can effectively predict the occurrence of weaning failure.

Effect of continuous renal replacement therapy adjuvant to broad-spectrum enzyme inhibitors on the efficacy and inflammatory cytokines in patients with severe acute pancreatitis.

OBJECTIVE: To investigate the effect of continuous renal replacement therapy (CRRT) combined with ulinastatin, a broad-spectrum enzyme inhibitor, on the treatment effect and inflammatory mediator levels in patients with severe acute pancreatitis (SAP). METHODS: A total of 80 patients with SAP admitted to our hospital were divided into two groups according to a random number table, with 40 cases in the control group and 40 cases in the experimental group. The control group was treated with the broad-spectrum enzyme inhibitor ulinastatin, and the experimental group was treated with continuous renal replacement therapy (CRRT) in addition to the control group's treatment method. The clinical efficacy was evaluated. Serum inflammation indicators, critical illness-related scores, pancreatic microcirculation and coagulation indicators were also detected before and after treatment. RESULTS: After 14 days of continuous intervention, the total effective rate of the experimental group was 92.50%, and that of the control group was 75.00%, with statistical significance between the two groups (P<0.05). The expression of APN in the two groups' serum increased, and the other inflammatory indexes decreased. The experimental group's serum APN was higher than that of the control group, and the other inflammatory indexes were lower than those of the control group (all P<0.001). The two groups' critical illness-related scores were improved, and there was a difference between the two groups (P<0.05). The levels of BF and BV increased, while TTP levels decreased, and there was a difference between the experimental and control groups (all P<0.01). The coagulation indexes of the two groups of patients were all improved. Compared with the control group, the coagulation indexes of the experimental group were lower. There was a difference between the two groups (P<0.01). CONCLUSION: CRRT adjuvant to broad-spectrum enzyme inhibitor ulinastatin can significantly improve the inflammatory response, microcirculation, hypercoagulability and clinical treatment efficacy in patients with severe acute pancreatitis.

[Development of a new FTIR car-borne oil content analyzer].

Turlun structure was introduced into the Fourier transform interference system in the present paper in order to decrease the size and cost of the whole system and to increase the ability of vibration resistance to meet the requirement of car-borne outdoor monitoring application. The speed deviation of the voice coil motor (VCM) driving system, which is based on feedback of interference wavelength of reference laser and DSP control, is within 5%. The standard oil sample was used to test the performance of the spectrometer. The result shows that the highest resolution of the FTIR oil detector is 2 cm(-1), and the SNR reached 2000:1. Compared with old grating spectrometers, thiskind of instrument is faster, and the quality of spectrum was increased significantly. The portable feature of this instrument will be more convenient for the water pollution detection in field