[Effects of PFOS on inflammatory factors in human placental trophoblast cells].

Objective: To investigate the effect of perfluorooctane sulfonate (PFOS) on inflammatory factors in human placental trophoblast (HTR-8/Svneo) cells. Methods: HTR-8/Svneo cells were exposed to different concentrations of PFOS (0, 0.01, 0.1, 1.0 mg/L) for 24 h, and the cell survival rates were measured by CCK8. Secretion levels of interleukin-6 (IL-6) , tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10) were detected by ELISA. The mRNA expressions of IL-6, TNF-α and IL-10 were detected by fluorescence quantitative PCR. One-way ANOVA was used to analyse the expressions of inflammatory factors. Results: Compared with the control group, the survival rates of 0.1 and 1.0 mg/L PFOS groups were significantly decreased (P<0.05) . Compared with the control group, the secretion levels of IL-6 were decreased in the 0.01, 0.1 and 1.0 mg/L PFOS groups (P<0.05) , the concentrations of TNF-α were increased in the 0.01 and 1.0 mg/L PFOS groups (P<0.05) , and the concentrations of IL-10 were increased in the 0.1 and 1.0 mg/L PFOS groups (P<0.05) . Compared with the control group, the expressions of IL-6 mRNA were increased in the 0.1 and 1.0 mg/L PFOS groups (P<0.05) , and the expressions of IL-10 mRNA were decreased in the 0.01 mg/L, 0.1 mg/L and 1.0 mg/L PFOS groups (P<0.05) . Conclusion: PFOS can induce changes in the secretion levels of inflammatory cytokines in HTR-8/Svneo cells, resulting in decreased activity of placental trophoblast cells and abnomal placental function.

[Effects of intrauterine exposure of perfluoroalkyl and polyfluoroalkyl substances on growth and development of offspring].

Perfluoroalkyl and polyfluoroalkyl substances (PFASs) are a new subgroup of the persistent organic pollutants that have been widely used in industrial production and daily life for over 60 years. The presence of PFASs can be detected in rivers, soil, humans and wildlifes. Studies have shown that PFASs can induce multi-system toxicity in laboratory animals, including liver toxicity, neurotoxicity, reproductive and developmental toxicity, endocrine toxicity and immunotoxicity. PFASs are closely related to the birth outcomes, growth and development of the offspring. PFASs primarily impair the health of the offspring by regulating peroxisome proliferators-activated receptors pathways, interfering with estrogen, affecting the thyroid system, glucocorticoids, inflammatory responses, and DNA methylation. Thus, more large-scale longitudinal cohort studies should be conducted and more in-depth potential mechanisms of action should be explored to clarify the relationship between intrauterine exposure of PFASs and birth outcomes, growth and development of offspring.

Epithelial barrier dysfunctions in atopic dermatitis: a skin-gut-lung model linking microbiome alteration and immune dysregulation.

BACKGROUND: Atopic dermatitis is a systemic disorder characterized by abnormal barrier function across multiple organ sites. Causes of epidermal barrier breakdown are complex and driven by a combination of structural, genetic, environmental and immunological factors. In addition, alteration in microflora diversity can influence disease severity, duration, and response to treatment. Clinically, atopic dermatitis can progress from skin disease to food allergy, allergic rhinitis, and later asthma, a phenomenon commonly known as the atopic march. The mechanism by which atopic dermatitis progresses towards gastrointestinal or airway disease remains to be elucidated. OBJECTIVES: This review addresses how epithelial dysfunction linking microbiome alteration and immune dysregulation can predispose to the development of the atopic march. METHODS: A literature search was conducted using the PubMed database for relevant articles with the keywords 'atopic dermatitis', 'epithelial barrier', 'skin', 'gut', 'lung', 'microbiome' and 'immune dysregulation'. RESULTS: Initial disruption in the skin epidermal barrier permits allergen sensitization and colonization by pathogens. This induces a T helper 2 inflammatory response and a thymic stromal lymphopoietin-mediated pathway that further promotes barrier breakdown at distant sites, including the intestinal and respiratory tract. CONCLUSIONS: As there are no immediate cures for food allergy or asthma, early intervention aimed at protecting the skin barrier and effective control of local and systemic inflammation may improve long-term outcomes and reduce allergen sensitization in the airway and gut.

[Isolation and structure elucidation of qianhucoumarin A].

Seven compounds were isolated from the traditional Chinese Medicine Qianhu, roots of Peucedanum praeruptorum Dunn. Their structures were identified as psoralen (II), 5-methoxypsoralen (III), 8-methoxypsoralen (IV), pteryxin (V), peucedanocoumarin II (VI), beta-sitosterol (VII) and qianhucoumarin A (VIII) by physico-chemical constants and spectral analyses (UV, IR, 1HNMR, 13CNMR, EIMS). VIII is a new compound with the structure of 3'(R)-hydroxy-4'(R)-tigloyloxy-3',4'-dihydroseslin. Its absolute configurations were determined by chemical correlation with known compounds. II, III, IV and VII were first isolated from this plant.

[The isolation and identification of qianhucoumarin B and qianhucoumarin C from Peucedanum praeruptorum].

Five compounds were isolated from the traditional Chinese medicine Qianhu, roots of Peucedanum praeruptorum. Their structures were identified as 3'-angeloyloxykhellactone (IX), qianhucoumarin B (X), qianhucoumarin C (XI), Pd-III (XIII) and peucedanocoumarin III (XV). Among them, X and XI are new compounds, their absolute configurations were elucidated by chemical correlation with khellactones, as 3'(S)-acetoxy-4'(S)-hydroxy-3',4'-dihydroseselin(X) and 3'(S)-hydroxy-4'(S)-acetoxy-3',4'-dihydroseselin (XI). Compound IX was isolated from plants for the first time.

[Studies on anthraquinones from the roots of rubia cordifolia l].

Seven anthraquinones were isolated from the ethyl alcohol extracts of the roots of Rubia Cordifolia L. By means of spectrometric data combined with physico-chemical properties, six of them were identified as 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone (I), 1-hydroxy-9,10-anthraquinone(II), 1,2,4-trihydroxy-9,10-anthrequinone(III), 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-beta-D-glucoside(IV), 1,2-dijhydroxy-9,10-anthraquinone-2-O-beta-D-xylosyl(1-->6)-beta-D -glucoside(V) and 1,3-dihydroxy-2-hydroxymethyl1-9,10-anthraquinone-3-O-beta-D- xylosyl(1-->6)-beta-D-glucoside(VI). VII is a new compound. Its structure was elucidated to be 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-beta-D-xylosyl(1-->2)-b eta-D- (6'-O-acetyl)glucoside.

[New monoterpenoids from Cynanchum hancockianum].

Five monoterpenoids have been isolated from the roots of Cynanchum hancockianum (Maxim) AI. Iljinski. Their structures were determined on the basis of spectral evidence. The three new compounds are: 4-p-menthane-1,7,8-triol (III), neohancoside A (IV), neohancoside B (V), and the two knowns: 4-p-menthene-1-8, 9-diol (I), and 4-p-menthane-1,8, 9-triol (II). Compound V was purified by acetylation.

[Studies on naphthoic acid esters from the roots of Rubia cordifolia L].

Four naphthoic acid esters including a new compound were isolated from the roots of Rubia cordifolia L. The new one was named as rubilactone and its structure was elucidated as 3'-carbomethoxy-4'-hydroxy-naphtho[1',2'-2,3] pyran-6-one (I) based on the physicochemical properties and spectrometric analyses (UV, IR, MS, 1HNMR and 13CNMR). The other three were 3'-carbomethoxy-4'-hydroxy-naphtho [1',2'-2,3] furan (II), dihydromollugin (III) and 3-carbomethoxy-2-(3'-hydroxy)isopentyl-1,4-naphthohydroquinone-1-O -beta-D-glucoside (IV).

[C21 steroidal constituents from Cynanchum hancockianum].

Two new compounds hancogenin B (V) and hancoside A (VI) and four known compounds glucogenin C (I), cynatratoside A (II), glaucogenin A (III) and anhydrohirundigenin (IV) were isolated from the roots of Cynanchum hancockianum (Maxim) Al. Iljinski. Their structures were identified on the basis of spectral evidence. The fragmentation ways of 13:14, 14:15-secopregnenes in EIMS were outlined and the antitumor activity of II and the antiendotoxic activity of VI were also preliminarily tested in vitro.

[Novel triterpenoids from Cynanchum hancockianum].

Four new triterpenoids have been isolated from the petroleum ether extract of Cyananchum hancockianum (Maxim) Al. Iljinski (Asclepiadaceae). On the basis of spectral data (1HNMR, 13CNMR, 1H-1H COSY, 1H-13C COSY, longrange 1H-13C COSY, MS, X-Ray analysis) and chemical connections, they were identified as hancockinol (Ia), hancolupenol (IIa), hancolupenone (IIc), and hancolupenol octacosanate (IId). They are of new skeletons with B/C cis, C/D trans, D/E cis junctures for Ia and with A/B trans, C/D trans, D/E cis junctures for IIa, IIc and IId.

[Studies on antibacterial constituents from the roots of Rubia cordifolia L].

Twelve compounds were isolated from the roots of Rubia cordifolia L.. Nine of them were established by chemical and spectroscopic methods to be alizarin (I),1-hydroxy-2-methyl-9,10-anthraquinone(II),1,3,6-trihydroxy-2-methyl- 9,10-anthra-quinone-3-O-(6'-O-acetyl)-alpha-L-rhamnosyl(1--- -2)-beta-D- glucoside(III),1,3,6-trihydroxy-2-methyl-9,10-anthraqueinone-3-O-a lpha- L-rhamnosyl(1----2)-beta-D-glucoside(IV),1,3,6-trihydrozy-2- methyl-9,10- anthraquinone-3-O-(6'-O-acetyl)-beta-D-glucoside(V),2-carbomethyoxy++ +-3-prenyl- 1,4-naphthohydroquinone di-beta-D-glucoside(VI),rubimallin(VII),beta- sitosterol(VIII) and daucosterol(IX), among them, V is a nwe compound and IX was obtained from this genus for the first time, III, VI and VII showed certain antibacterial activities.

[Studies on the structure of a new ellagic acid glycoside from the root-sprouts of Agrimonia pilosa Ledeb].

Four compounds were isolated from the root-sprouts of Agrimonia pilosa Ledeb. On the basis of physico-chemical properties, spectroscopy (UV, IR, NMR, MS) and chemical degradation, a new compound was elucidated as ellagic acid-4-O-beta-D-xylopyranoside (XV), and three known compounds were identified as agrimonolide (XIII), tormentic acid (XIV), and ellagic acid (XVI).

[Studies on the structure of a new flavanonol glucoside of the root-sprouts of Agrimonia pilosa Ledeb].

Three compounds were isolated from benzene and acetone extracts of the root-sprouts of Agrimonia pilosa Ledeb. On the basis of physicochemical properties, spectroscopy (UV, IR, NMR, MS, CD, GC) and chemical degradation, two known compounds were identified as palmitic acid (VIII) and daucosterol (IX), one new compound was elucidated as (2S,3S)-(-)-taxifolin-3-O-beta-D-glucopyranoside (X).

[Identification of flavonoids from Oxytropis glabra DC].

The structures and identification of five flavonoids from Oxytropis glabra are described. The five flavonoids are kaempferol, quercetin, kaempferol-3-beta-D-glucoside, kaempferol-7-alpha-L-rhamnoside and kaempferol-3-diglucoside. All of them were isolated from this plant for the first time.

[Studies on antibacterial constituents from Gerbera anandria (L.) Sch Bip. IV].

Thirteen compounds have been isolated from Gerbera anandria (L.) Sch Bip. Among them, three new compounds were elucidated by chemical correlations and spectral analyses. They are 3.8-dihydroxy-4-methoxy-coumarin (XIX), 3,8-dihydroxy-4-methoxy-2-oxo-2H-1-benzopyran-5-carboxylic acid (XVII), and 5,8-dihydroxy-7-(4-hydroxy-5-methyl-coumarin-3-)coumarin (XXI).

[Studies on the structure of a new isocoumarin glucoside of the root sprouts of Agrimonia pilosa Ledeb].

Trans-p-hydroxycinnamic esters VI and an isocoumarin glucoside VII were isolated from the benzene and acetone extracts of the root sprouts of Agrimonia pilosa Ledeb. On the basis of physicochemical properties, spectral data (UV, IR, NMR, MS, CI-MS-MS), GC and chemical degradation, VI was elucidated as trans-p-hybroxycinnamic esters of n-C22, n-C24-32, n-C34, alcohols, VII was elucidated as agrimonolide-6-Q-beta-D-glucopyranoside. VII and trans-p-hydroxycinnamic esters of n-C29-32 and n-C34 alcohols are new compounds.

[A new minor saponin from the leaves of Panax ginseng C. A. Meyer].

Two minor compounds isolated from the leaves of Panax ginseng C. A. Meyer were characterized as 20(R)-protopanaxadiol (I) and 3 beta, 6 alpha, 12 beta-trihydroxydammar-20 (22), 24-diene-6-O-alpha-L-rhamnosyl-(1----2)-beta-D-glucopyranoside (II) on the basis of spectral analysis and chemical evidences. I was isolated for the first time from the leaves; II was shown to be a new saponin and was named as ginsenoside-Rg4.

[An empirical correlation between optical rotation and absolute configuration of optically active alpha-methylbutyrylphloroglucinols and its synthesis].

Fourteen new compounds were synthesized of which twelve are (S)-(+)-alpha-methylbutyrylphloroglucinols. On the basis of optical rotation data and theoretical analysis, an empirical correlation between optical rotation and absolute configuration of optically active alpha-methylbutyrylphloroglucinols has been established by means of Brewster's rule of atomic asymmetry. It can be served as a means of absolute configuration assignment of optically active alpha-methylbutyrylphloroglucinols.

[Studies on the chemical constituents from the root-sprouts of Agrimonia pilosa Ledeb].

Five compounds were isolated from the petroleum ether extract of the root-sprouts of Agrimonia pilosa Ledob. A new compound, (R)-(-)-agrimol B (III), was elucidated by means of physical and chemical properties, spectroscopy (MS, IR, PMR, UV) and total synthesis. Four known compounds were identified as agrimophol (I), n-nonacosane (II), beta-sitosterol (IV) and pseudo-aspidin (V), the last one being previously known only as a synthetic entity. (S)-(+)-Agrimol B also was synthesized.