[Identification of B(A) Blood Group Serology Combined with Molecular Biology and Blood Transfusion Strategy].

OBJECTIVE: To analyze the reasons for ABO blood group forward typing and reverse typing incompatibility of the proband by serological and molecular biological tests, so as to clarify the blood group and genetic rules and determine the reasonable transfusion strategy. METHODS: On the basis of serological testing results, PCR-SSP method was utilized for the ABO exon sequencing in the peripheral blood of the proband and 5 family members, and blood group results were comprehensively analyzed. RESULTS: The serological results of ABO blood group of the proband were incompatibility, and the molecular biological test results showed that there was a c.700C>G mutation compared with B101, which was consistent with B(A)02 subtype, and the genotype was B(A)02/O02. The results of the elder brother was the same as the proband. The nephew of the proband also detected c.700C>G, genotype A102/B(A) 02. CONCLUSION: When the result of standard serological test for ABO blood group incompatibility occurs, using molecular biology detection technology to explore mutation is an effective method to confirm ABO subtype, and ensures the safety of clinical blood transfusion.

[Multicenter controlled study on abdominal acupuncture for treatment of nerve root type cervical spondylosis].

OBJECTIVE: To observe the therapeutic effect of abdominal acupuncture on nerve root cervical spondylosis. METHODS: Three hundred cases were randomly assigned to a treatment group and a traction control group. The treatment group were treated with abdominal acupuncture at an acupoint prescription, i.e. Zhongwan (CV 12), Guanyuan (CV 4) and others were selected. The control group were treated with suspended traction by a cervical vertebral extraction belt at pillow-jaw sitting position. The short-term and long-term therapeutic effects were observed at the end of therapeutic course and 90 days after the end of treatment, respectively. RESULTS: The total short-term and the long-term therapeutic effects were 97.3% and 92.7% in the treatment group, and 82.0% and 82.0% in the control group, respectively, with significant differences between the two groups (P < 0.01). CONCLUSION: Abdominal acupuncture has better short-term and long-term therapeutic effects with short therapeutic course.