Lower extremity peripherally inserted central catheter placement ectopic to the ascending lumbar vein: A case report.

BACKGROUND: Peripherally inserted central catheters (PICCs) are an essential infusion route for oncology patients receiving intravenous treatments, but lower extremity venipuncture is the preferred technique for patients with superior vena cava syndrome (SVCS). We report the case of a patient with a lower extremity PICC ectopic to the ascending lumbar vein, to indicate and verify PICC catheterisation in the lower extremity is safe and feasible. And hope to provide different perspectives for clinical PICC venipuncture to get the attention of peers. CASE SUMMARY: On 24 August 2022, a 58-year-old male was admitted to our department due to an intermittent cough persisting for over a month, which worsened 10 d prior. Imaging and laboratory investigations suggested the patient with pulmonary malignancy and SVCS. Chemotherapy was not an absolute contraindication in this patient. Lower extremity venipuncture is the preferred technique because administering upper extremity venous transfusion to patients with SVCS can exacerbate oedema in the head, neck, and upper extremities. The patient and his family were informed about the procedure, and informed consent was obtained. After successful puncture and prompt treatment, the patient was discharged, experiencing some relief from symptoms. CONCLUSION: Inferior vena cava catheterisation is rare and important for cancer patients with SVCS, particularly in complex situations involving ectopic placement.

Warm Acupuncture Reduces Pain and Inflammation in Rats with Lumbar Disc Herniation Induced by Autologous Nucleus Pulposus Transplantation via Regulating p38MAPK/NF-κB Pathway.

Background: : Warm acupuncture (WA) has analgesic and anti-inflammatory effects. However, the underlying mechanism of these effects remain unclear. Objectives: : To explore the analgesic and anti-inflammatory effects of WA and the potential underlying mechanism in male Sprague-Dawley rats with non-compressive lumbar disk herniation (LDH) caused by autologous nucleus pulposus (NP) transplantation. Methods: : We used low-frequency (2 Hz) electrical stimulation and WA (40℃) to treat GB30 and BL54 acupoints in rats for 30 mins per day. We monitored the paw withdrawal threshold of rats during the experiment and measured serum cytokine levels using commercial kits. Dorsal root ganglion (DRG) tissue pathology was analyzed via H&E staining. We used qRT-PCR to measure the mRNA expression levels of IL-1ß, IL-6, and TNF-α genes in DRG. Western blot was used to analyze the expression levels of IL-1ß, IL-6, TNFα, P-p38MAPK, p38MAPK, P-IκBα, IκB α, and NF-κB p65 proteins. Results: : WA treatment significantly increased the pain threshold of rats, reduced serum IL-6, PEG2, NO, SP, NP-Y, and MMP-3 levels, and effected histopathological improvements in the DRG in rats. Moreover, WA treatment significantly downregulated the expression levels of inflammation-associated genes (Il-1ß, Il-6, and Tnf-α) and proteins (IL-1ß, IL-6, TNF-α, P-p38MAPK, P-IκBα, and NF-κB p65) in the DRG of non-compressive LDH rats. Conclusion: : WA can alleviate pain and inhibit inflammatory response in rats with non-compressive LDH caused by autologous NP transplantation, and these effects are likely associated with the inhibition of the p38MAPK/NF-κB pathway.

Optimizing the tip position of a peripherally inserted central venous catheter under a multidisciplinary team approach in a patient with mirror-image dextrocardia: a case report.

Peripherally inserted central venous catheters (PICCs) are widely used in cardiology because they are convenient, effective, and safe. However, PICC implantation in patients with mirror-image dextrocardia who have thoracic tumors has not yet been reported. In this case report, we describe a 46-year-old patient with lung cancer who had a thoracic inclination and left pulmonary artery compression of the superior vena cava. PICC implantation under B-ultrasound guidance was planned. Because of the anatomical differences caused by mirror-image dextrocardia, we investigated the optimal position and measurement method for the tip of the PICC according to the compression site of the vascular lumen through a multidisciplinary team approach. Electrocardiography-assisted tip positioning combined with postoperative chest X-ray positioning was performed for accurate positioning.

New species and newly recorded species of the genus Agonopterix Hübner, [1825] (Lepidoptera: Depressariidae) from China.

Thirteen new species of the genus Agonopterix Hübner are described: A. basinigra sp. nov., A. cochleata sp. nov., A. epunctata sp. nov., A. foliiformis sp. nov., A. gibbosa sp. nov., A. hypodroma sp. nov., A. introrsa sp. nov., A. magnimacularis sp. nov., A. nephimacula sp. nov., A. nivimacularis sp. nov., A. parallela sp. nov., A. simipullella sp. nov. and A. sphaeroidea sp. nov. and ten species are recorded for the first time from China: A. abditella Hannemann, 1959, A. angelicella (Hübner, 1813), A. arctica (Strand, 1902), A. arenella (Denis & Schiffermüller, 1775), A. bipunctosa (Curtis, 1850), A. broennoeensis (Strand, 1920), A. kaekeritziana (Linnaeus, 1767), A. kirgizella Lvovsky, 2001, A. ochrocephala Saito, 1980 and A. septicella (Snellen, 1884). Images of both adults and genitalia are provided.

Oncogenic KRAS signaling drives evasion of innate immune surveillance in lung adenocarcinoma by activating CD47.

KRAS is one of the most frequently activated oncogenes in human cancers. Although the role of KRAS mutation in tumorigenesis and tumor maintenance has been extensively studied, the relationship between KRAS and the tumor immune microenvironment is not fully understood. Here, we identified a role of KRAS in driving tumor evasion from innate immune surveillance. In samples of lung adenocarcinoma from patients and Kras-driven genetic mouse models of lung cancer, mutant KRAS activated the expression of cluster of differentiation 47 (CD47), an antiphagocytic signal in cancer cells, leading to decreased phagocytosis of cancer cells by macrophages. Mechanistically, mutant KRAS activated PI3K/STAT3 signaling, which restrained miR-34a expression and relieved the posttranscriptional repression of miR-34a on CD47. In 3 independent cohorts of patients with lung cancer, the KRAS mutation status positively correlated with CD47 expression. Therapeutically, disruption of the KRAS/CD47 signaling axis with KRAS siRNA, the KRASG12C inhibitor AMG 510, or a miR-34a mimic suppressed CD47 expression, enhanced the phagocytic capacity of macrophages, and restored innate immune surveillance. Our results reveal a direct mechanistic link between active KRAS and innate immune evasion and identify CD47 as a major effector underlying the KRAS-mediated immunosuppressive tumor microenvironment.

Bedside ultrasonic localization of the nasogastric tube in a patient with severe COVID-19: A case report.

BACKGROUND: The indwelling nasogastric tube is commonly used for supplying enteral nutrition to patients who are unable to feed themselves, and accurate positioning is essential in the indwelling nasogastric tube in the body of the aforementioned patients. In clinical practice, abdominal radiography, auscultation, and clinical determination of the pH of the gastric juice are routinely used by medical personnel to determine the position of the tube; however, those treatments have proved limitations in specific cases. There are few case reports on the precise positioning of the nasogastric tube in patients with coronavirus disease 2019 (COVID-19), for whom a supply of necessary nutrition support is significant throughout the process of treatment. CASE SUMMARY: A 79-year-old patient, diagnosed with COVID-19 at the stage of combined syndromes with severe bacterial lung infection, respiratory failure, multiple co-morbidities, and a poor nutritional status, was presented to us and required an indwelling nasogastric tube for enteral nutrition support. After pre-treatment assessments including observation of the patient's nasal feeding status and examination of the nasal septal deviation, inflammation, obstruction, nasal leakage of cerebrospinal fluid, and other disorders that might render intubation inappropriate, we measured and marked the length of the nasogastric tube to be placed and delivered the tube to the intended length in the standard manner. Then further scrutiny was conducted to ensure that the tube was not coiled in the mouth, and gentle movements were made to avoid damage to the esophageal mucosa. However, back draw of the gastric juice using an empty needle failed, and the stethoscope could not be used for auscultation due to the specific condition presented by the internal organs of the patient, and the end of the tube was placed in saline with no bubbles spilling out. Therefore, it was not possible to determine whether the nasogastric tube was placed exactly in the stomach and no nutrient infusion was performed for the time being. Subsequently, the ultrasound probe was utilized to view the condition of the patient's stomach, where the nasogastric tube was found to be translucent and running parallel to the esophagus shaped as "=". The pre-conditions were achieved and 100 mL nutritional fluid was fed to the patient, who did not experience any discomfort throughout the procedure. His vital signs were stable with no adverse effects. CONCLUSION: We achieved successfully used ultrasound to position the nasogastric tube in a 79-year-old patient with COVID-19. The repeatable ultrasound application does not involve radiation and causes less disturbance in the neck, making it advantageous for rapid positioning of the nasogastric tube and worthy of clinical promotion and application.

Taxonomy of the genus Meleonoma Meyrick, 1914 (Lepidoptera: Autostichidae) from China (IV), with descriptions of twelve new species.

Twelve new species of the genus Meleonoma Meyrick are described. Meleonoma aculeolata sp. nov. and M. rectivalva sp. nov. belong to the acutiuscula species-group; M. robustijuxta sp. nov. and M. varilobata sp. nov. belong to the jigongshanica species-group; M. basitriangula sp. nov., M. biprocessa sp. nov., M. curvicornuta sp. nov., M. dilatilobata sp. nov., M. globulosa sp. nov., M. prospina sp. nov., M. sinuata sp. nov. and M. unijugata sp. nov. belong to the puncticulata species-group. Images of both adults and genitalia are provided.

Study of the genus Ethmia Hübner, [1819] (Lepidoptera: Ethmiidae) from China, with descriptions of six new species.

Six new species of Ethmia Hübner [1819] are described and illustrated from China: E. adunatimacula sp. nov., E. anfracta sp. nov., E. anomala sp. nov., E. corrugata sp. nov., E. rectiuga sp. nov. and E. transtimacula sp. nov. Two species (E. anatiformis Kun, 2001 and E. vietmiella Kun, 2001) are newly recorded for China; one species (E. crocosoma Meyrick, 1914) is newly recorded for the Mainland China. The male of E. susa Kun & Szabóky 2000 and the females of E. antennipilosa Wang & Li, 2004, E. vietmiella Kun, 2001 and E. yeni Kun, 2001 are reported for the first time. Images of adults and genitalia are provided.

Study of the genus Meleonoma Meyrick, 1914 (Lepidoptera: Autostichidae) from China (III), with descriptions of eighteen new species.

Eighteen new species of the genus Meleonoma Meyrick are described: M. arcivalvata sp. nov., M. chongqingensis sp. nov., M. curvativa sp. nov., M. curvitaeniana sp. nov., M. dilatifasciata sp. nov., M. fasciptera sp. nov., M. globoidea sp. nov., M. graciliclavata sp. nov., M. grandivalvula sp. nov., M. lunata sp. nov., M. medispinea sp. nov., M. proapicalis sp. nov., M. raphidacantha sp. nov., M. sinuaclavata sp. nov., M. tenuiclavata sp. nov., M. tetrodonta sp. nov. M. ventridentata sp. nov. and M. ventrisinuata sp. nov. The female of M. similifloralis (Wang, 2006) is described for the first time. Images of both adults and genitalia are provided. All species are divided into two species-groups, the dentivalvata species-group and the fasciptera species-group. A key to each group and maps showing the distribution of each group in China are given.

Decreased inhibition of exosomal miRNAs on SARS-CoV-2 replication underlies poor outcomes in elderly people and diabetic patients.

Elderly people and patients with comorbidities are at higher risk of COVID-19 infection, resulting in severe complications and high mortality. However, the underlying mechanisms are unclear. In this study, we investigate whether miRNAs in serum exosomes can exert antiviral functions and affect the response to COVID-19 in the elderly and people with diabetes. First, we identified four miRNAs (miR-7-5p, miR-24-3p, miR-145-5p and miR-223-3p) through high-throughput sequencing and quantitative real-time PCR analysis, that are remarkably decreased in the elderly and diabetic groups. We further demonstrated that these miRNAs, either in the exosome or in the free form, can directly inhibit S protein expression and SARS-CoV-2 replication. Serum exosomes from young people can inhibit SARS-CoV-2 replication and S protein expression, while the inhibitory effect is markedly decreased in the elderly and diabetic patients. Moreover, three out of the four circulating miRNAs are significantly increased in the serum of healthy volunteers after 8-weeks' continuous physical exercise. Serum exosomes isolated from these volunteers also showed stronger inhibitory effects on S protein expression and SARS-CoV-2 replication. Our study demonstrates for the first time that circulating exosomal miRNAs can directly inhibit SARS-CoV-2 replication and may provide a possible explanation for the difference in response to COVID-19 between young people and the elderly or people with comorbidities.

Sperm microRNAs confer depression susceptibility to offspring.

Evidence that offspring traits can be shaped by parental life experiences in an epigenetically inherited manner paves a way for understanding the etiology of depression. Here, we show that F1 offspring born to F0 males of depression-like model are susceptible to depression-like symptoms at the molecular, neuronal, and behavioral levels. Sperm small RNAs, and microRNAs (miRNAs) in particular, exhibit distinct expression profiles in F0 males of depression-like model and recapitulate paternal depressive-like phenotypes in F1 offspring. Neutralization of the abnormal miRNAs in zygotes by antisense strands rescues the acquired depressive-like phenotypes in F1 offspring born to F0 males of depression-like model. Mechanistically, sperm miRNAs reshape early embryonic transcriptional profiles in the core neuronal circuits toward depression-like phenotypes. Overall, the findings reveal a causal role of sperm miRNAs in the inheritance of depression and provide insight into the mechanism underlying susceptibility to depression.

Study of the genus Meleonoma Meyrick, 1914 (Lepidoptera: Autostichidae) from China, with descriptions of twenty-one new species (II).

Twenty-one new species of the genus Meleonoma Meyrick are described: M. acutata sp. nov., M. annulignatha sp. nov., M. artivalva sp. nov., M. basiprocessa sp. nov., M. bicornea sp. nov., M. bidigitata sp. nov., M. circinans sp. nov., M. compacta sp. nov., M. cuneata sp. nov., M. forcipata sp. nov., M. ledongensis sp. nov., M. longihamata sp. nov., M. mecobursoides sp. nov., M. microdonta sp. nov., M. papillisetosa sp. nov., M. parallela sp. nov., M. recticostata sp. nov., M. segregnatha sp. nov., M. sinuicosta sp. nov., M. taiwanensis sp. nov. and M. taeniophylla sp. nov. The male of M. flavilineata Kitajima et Sakamaki, 2019 is described for the first time. Images of both adults and genitalia are provided. All species are divided into two species-groups, and a key to each group as well as a map showing the distribution of each group in China are given.

Genus Meleonoma Meyrick, 1914 (Lepidoptera, Autostichidae) from Hainan Island, China, with descriptions of sixteen new species.

Sixteen new species of the genus Meleonoma Meyrick, 1914 from Hainan Island, China are described: M. apicicurvata Wang, sp. nov., M. apicirectangula Wang, sp. nov., M. bicuspidata Wang, sp. nov., M. bidentata Wang, sp. nov., M. conica Wang, sp. nov., M. hainanensis Wang, sp. nov., M. latiunca Wang, sp. nov., M. linearis Wang, sp. nov., M. magnidentata Wang, sp. nov., M. ornithorrhyncha Wang, sp. nov., M. parilis Wang, sp. nov., M. pectinalis Wang, sp. nov., M. puncticulata Wang, sp. nov., M. quadritaeniata Wang, sp. nov., M. robustispina Wang, sp. nov. and M. rostellata Wang, sp. nov. Images of adult dorsal habitus and genitalia of the new species are provided. A map showing the collecting localities and photos of the habitat where the specimens were collected are provided, along with two maps showing the distribution of each species.

Study of the genus Meleonoma Meyrick, 1914 (Lepidoptera: Autostichidae) from China, with descriptions of fifteen new species.

Fifteen new species of the genus Meleonoma Meyrick are described: M. anisodonta sp. nov., M. catenata sp. nov., M. dilativalva sp. nov., M. dorsibacilliformis sp. nov., M. lanceolata sp. nov., M. longaedeaga sp. nov., M. longiprocessa sp. nov., M. lunulata sp. nov., M. papillata sp. nov., M. parvissima sp. nov., M. rugulosa sp. nov., M. tenuiuncata sp. nov., M. tianmushana sp. nov., M. triacantha sp. nov. and M. ventrospinosa sp. nov.; one species, M. aridula (Meyrick, 1910), is newly recorded from China; and the female of M. microbyrsa (Wang, 2003) is described for the first time. Images of both adults and genitalia are provided. All species are divided into two species-groups, and a key to each group as well as a map showing the distribution of each group in China are given.

Taxonomic review of the genus Meleonoma Meyrick (Lepidoptera: Autostichidae), with a checklist of all the described species.

Meleonoma Meyrick, 1914 is treated as a genus of the family Autostichidae based on the recent study. Fifty species are transferred from the genus Cryptolechia to Meleonoma, and eight Meleonoma species groups are proposed for the taxonomic convenience based on both molecular data and morphological study. Eight species groups include: the malacobyrsa group, the segregnatha group, the annulignatha group, the fasciptera group, the jigongshanica group, the dentivalvata group, the facialis group, and the acutiuscula group. Diagnostic characters and representative illustrations of each species group are provided. A discussion on the proposed species groups is given, and a checklist of all the 88 described Meleonoma species is provided.

Upregulation of Spinal Voltage-Dependent Anion Channel 1 Contributes to Bone Cancer Pain Hypersensitivity in Rats.

Voltage-dependent anion channel 1 (VDAC1) is thought to contribute to the progression of tumor development. However, whether VDAC1 contributes to bone cancer pain remains unknown. In this study, we found that the expression of VDAC1 was upregulated in the L2-5 segments of the spinal dorsal horn at 2 and 3 weeks after injection of tumor cells into the tibial cavity. Intrathecal injection of a VDAC1 inhibitor significantly reversed the pain hypersensitivity and reduced the over-expression of Toll-like receptor 4 (TLR4). Intrathecal injection of minocycline, an inhibitor of microglia, also attenuated the pain hypersensitivity of rat models of bone cancer pain. These results suggest that VDAC1 plays a significant role in the development of complicated cancer pain, possibly by regulating the expression of TLR4.