Sequential treatment of concomitant odontoid fracture and lower cervical fracture-dislocation: A case report.

INTRODUCTION AND IMPORTANCE: To report the sequential treatment of a Type II odontoid fracture combined with a severe lower cervical (C6-7) fracture-dislocation featuring bilateral facet joint interlocking. CASE PRESENTATION: A 58-year-old male who had suffered an injury in a car accident, He presented neck pain and extremity paralysis. His neurological function was classified as per the American Spinal Injury Association (ASIA) impairment scale as Grade A, indicating complete deficits below the C6 spinal cord level. A cervical CT scan and magnetic resonance image showed a type II odontoid fracture, C6 slipped anteriorly, C6-7 bilateral facet joint fracture and interlocking, slightly compression change of C7 upper endplate. CLINICAL DISCUSSION: Emergency closed reduction using cranial tong traction was success 6 h after the injury. A subsequent CT scan proved the successful reduction of bilateral facet joint dislocations and the odontoid fracture. After careful overall assessment, anterior cervical decompression and fusion (ACDF) was performed at C5-6 and C6-7 segments three days later，while odontoid fracture was treated conservatively. At the 4 months follow-up, a CT scan demonstrated solid bone fusion at C5-6, C6-7 segments, along with successful healing at the odontoid fracture site. However, spinal cord was necrosis at C5-7 segments, and the patient's neurological function had no improvement. CONCLUSION: The initial closed reduction could restore the alignment and preliminary stability of cervical spine at sub-axial cervical fracture-dislocation segment as well as displaced odontoid fracture. This timely and effective closed reduction significantly diminished sequential surgical trauma and mitigated associated risks.

Molecular basis for substrate transport of Mycobacterium tuberculosis ABC importer DppABCD.

The type I adenosine 5'-triphosphate (ATP)-binding cassette (ABC) transporter DppABCD is believed to be responsible for the import of exogenous heme as an iron source into the cytoplasm of the human pathogen Mycobacterium tuberculosis (Mtb). Additionally, this system is also known to be involved in the acquisition of tri- or tetra-peptides. Here, we report the cryo-electron microscopy structures of the dual-function Mtb DppABCD transporter in three forms, namely, the apo, substrate-bound, and ATP-bound states. The apo structure reveals an unexpected and previously uncharacterized assembly mode for ABC importers, where the lipoprotein DppA, a cluster C substrate-binding protein (SBP), stands upright on the translocator DppBCD primarily through its hinge region and N-lobe. These structural data, along with biochemical studies, reveal the assembly of DppABCD complex and the detailed mechanism of DppABCD-mediated transport. Together, these findings provide a molecular roadmap for understanding the transport mechanism of a cluster C SBP and its translocator.

Overlooked mechanism of Pb immobilization on montmorillonite mediated by dissolved organic matter in manure compost.

In this study, three kinds of dissolved organic matter (DOM) derived from fresh chicken manure (FDOM), immature compost (IDOM) and mature compost (MDOM) were employed to compare their effects on Pb adsorption onto montmorillonite (MMT). The potential mechanism was revealed by characterization of mineral structure and calculation of interface force. The results demonstrated that the adsorption capacity (qmax) of Pb onto MMT was decreased by 14.3% and 29.8% in the presence of FDOM and IDOM, respectively, while increased by 44.4% in the presence of MDOM, resulting from the release or co-adsorption of DOM-Pb complexes. Parallel factor (PARAFAC) further indicated that Pb mainly bound to protein-like substances in FDOM and IDOM, and fulvic-like in MDOM. The X-ray diffraction (XRD) analysis proved that MDOM-Pb complex had a stronger ability to enter into the interlayer of MMT. The van der Waals force dominated the adsorption of FDOM-Pb and IDOM-Pb, while ligand exchange was involved in the case of MDOM-Pb. This study provided a comprehensive insight into the geochemical behavior of livestock manure and its compost as well as their interactions with heavy metal and soil mineral.

Preparation of Anti-Bacterial Cellulose Nanofibrils (CNFs) from Bamboo Pulp in a Reactable Citric Acid-Choline Chloride Deep Eutectic Solvent.

In this study, bamboo pulp was simultaneously fibrillated and esterified in one-pot citric acid−choline chloride deep eutectic solvent treatment. The results indicated that increasing the temperature and time promoted esterification, yielding 0.19 to 0.35 mmol/g of the carboxyl group in CNFs. However, increasing the temperature and time resulted in decreases in yields and the diameter of CNFs from 84.5 to 66.6% and 12 to 4 nm, respectively. Analysis of the anti-bacterial activities of CNFs suggested that the high carboxyl group content corresponded to the effective inhibition of Escherichia coli and Staphylococcus aureus Taking yield, size, carboxyl group content, and anti-bacterial activate into consideration, treatment at 120 °C for 24 h was the optimal condition, yielding 76.0% CNF with 0.31 mmol/g carboxyl groups with a diameter of 8 nm and the inhibition fof E. coli (81.7%) and S. aureus (63.1%). In addition, effect of different CNFs on characteristics of polyvinyl alcohol (PVA) films were investigated. The results indicated that CNF obtained from the optimal condition was a favorable additive for the composite film, which enhanced (74%) the tensile strength of composite film compared with the pure PVA film due to its considerable size and carboxyl group content. However, the composite films did not show an anti-bacterial activate as CNF.

Insight into interactions of heavy metals with livestock manure compost-derived dissolved organic matter using EEM-PARAFAC and 2D-FTIR-COS analyses.

Dissolved organic matter (DOM), as the most active ingredient in compost, directly determines the speciation and environmental behavior of HMs. Here, the binding properties of DOM derived from chicken-manure compost (CHM), cow-manure compost (COM) and pig-manure compost (PIM) with HMs were explored by analyses of Fluorescence excitation-emission matrix parallel factor (EEM-PARAFAC) and two-dimensional correlation Fourier transform infrared spectroscopy (2D-FTIR-COS). Results showed that the binding characteristics vary with origin of DOM and type of HMs. The fulvic-like component dominated the transformation of HMs speciation, and CHM-DOM had higher affinity with HMs and greater risk causing pollution due to its higher aromaticity, molecular weight and distribution of fluorescent components. Moreover, Cu(II) can efficiently bind to DOM with the stability constants (log kM) ranging from 4.53 to 5.38, followed by Pb(II) (3.34-3.57), whereas Cd(II) can hardly bind to DOM. The amide and polysaccharide were the predominant sites for HMs binding in CHM-DOM, and polysaccharide and phenolic in COM-DOM, while phenolic and amide in PIM-DOM, respectively. Although the proportion of protein-like components and non-fluorescent polysaccharides in DOM were low, their role in HMs binding should not be ignored. In brief, the environmental risk caused by livestock manure compost may originate from interactions between DOM and HMs.

Cadmium-induced phytotoxicity and tolerance response in the low-Cd accumulator of Chinese cabbage (Brassica pekinensis L.) seedlings.

In vegetable production, Chinese cabbage can readily accumulate cadmium (Cd) into its edible parts and exceed food safety standards. However, there are still some ecotypes that respond differently to cadmium stress. This study aimed to investigate the differences of Cd-induced (0, 10, 50, 100, 200 µM) response under hydroponic culture between two Chinese cabbage ecotypes which were promoted in northeastern China from the characteristics of biomass, uptake kinetic, accumulation, and initial oxidative stress. In this paper, it was confirmed that Jinfeng (JF) was a Cd-tolerant cultivar and had low Cd accumulation in edible part, while Qiutian (QT) was Cd-sensitive, exhibiting a faster Cd uptake rate but lacking effective Cd detoxication mechanisms, and was severely damaged by 10 µM Cd treatment. Conversely, even at a high Cd concentration of 200 µM, Jinfeng had weaker biomass inhibition, lower root Cd affinity, more difficult root-to-leaf translocation, and stronger antioxidant enzyme activity than Qiutian. In conclusion, Jinfeng can endure mild Cd stress (<10 µM), and Qiutian can be used as a Cd indicator. This study provides reliable materials and related data support for vegetable production in areas with mild Cd pollution.Novelty statement: This work further investigates the unique features of low-Cd accumulator in Chinese cabbage (Brassica pekinensis L.) seedlings as an interesting material for vegetable production in areas with mild Cd pollution. It also explains the differences between Cd-tolerant and Cd-sensitive cultivars under different cadmium stress levels and how these differences can alter their response. With the increase of Cd concentration, Cd-tolerant cultivars compared to Cd-sensitive cultivars showed less biomass decrease, lower accumulation, lower TF, more chemically stable Cd in roots and more active antioxidant enzymes under the same Cd stress level. With the development of seedlings, the uptake of Cd in roots and the translocation to the leaves were effectively restricted by the poor Cd affinity of roots, the conversion of Cd chemical forms and the promotion of antioxidase activities, in a Cd-tolerant low accumulator, Jinfeng.

Uniform remeshing with an adaptive domain: a new scheme for view-dependent level-of-detail rendering of meshes.

We present a new algorithm for view-dependent level-of-detail rendering of meshes. Not only can it effectively resolve complex geometry features similar to edge collapse-based schemes, but it also produces meshes that modern graphics hardware can render efficiently. This is accomplished through a novel hybrid approach: For each frame, we view-dependently refine the progressive mesh (PM) representation of the original mesh and use the output as the base domain of uniform regular refinements. The algorithm exploits frame-to-frame coherence and only updates portions of the output mesh corresponding to modified domain triangles. The PM representation is built using a custom volume preservation-based error function. A simple k-d tree enhanced jump-and-walk scheme is used to quickly map from the dynamic base domain to the original mesh during regular refinements. In practice, the PM refinement provides a view-optimized base domain for later regular refinements. The regular refinements ensure almost-everywhere regularity of output meshes, allowing optimization for vertex cache coherence and caching of geometry data in high-performance graphics memory. Combined, they also have the effect of allowing our algorithm to operate on uniform clusters of triangles instead of individual ones, reducing CPU workload.