ABSTRACT
Test systems for rapid detection of mycoplasmas in biological samples have been elaborated on the base of the polymerase chain reaction (PCR). Amplification of the conservative rDNA sequences was used for testing of cell cultures for mycoplasmal contamination. Mycoplasma pneumoniae detection system has been developed based on amplification of the species-specific DNA sequences. Inversions of some repeated sequences in the Mycoplasma pneumoniae genome make it possible to run the PCR with a single primer. The revealed spacer length polymorphism for 16S-23S rDNA operons can be used in the mycoplasmas identification.
Subject(s)
Gene Amplification , Mycoplasma Infections/diagnosis , Mycoplasma pneumoniae/genetics , Animals , Base Sequence , Birds , Cells, Cultured , DNA, Bacterial/genetics , Genes, Bacterial , Humans , Molecular Sequence Data , Mycoplasma Infections/microbiology , Polymerase Chain ReactionABSTRACT
It was found that phage phi kF77 is resistant to all known Pseudomonas aeruginosa restriction systems. Three types of mutants (dc-) which were unable to grow on different restrictive strains were isolated. All of them belong to one complementation group. Some of these mutations affected also the number of nicks in phage phi kF77 DNA and increased phage resistance to temperature treatment. It may be supposed that genes responsible for antirestriction mechanisms and introduction of nicks into DNA are connected in definite way.
