Individual dose reconstruction among residents living in the vicinity of the Semipalatinsk nuclear test site using EPR spectroscopy of tooth enamel.

Individual accumulated doses were determined by EPR spectroscopy of tooth enamel for 26 adult persons residing in territories adjacent to the Semipalatinsk Nuclear Test Site (SNTS). The absorbed dose values due to radiation from nuclear tests were obtained after subtracting the contribution of natural background radiation from the total accumulated dose. The determined dose values ranged up to 250 mGy, except for one person from Semipalatinsk city with a measured dose of 2.8 +/- 0.4 Gy. Increased dose values were determined for the individuals whose teeth were formed before 1962, the end of the atmospheric nuclear tests. These values were found to be significantly larger than those obtained for a group of younger residents of heavily exposed territories and the residents of territories not exposed to radioactive fallout. These increased dose values are consistent with those based on officially registered data for the Northeastern part of Kazakstan adjacent to SNTS, which was exposed to high levels of radioactive fallout from nuclear tests in period 1949-1962.

[New possibilities of the antibiotic use in the treatment of patients with acute cholecystitis]. / Novye vozmozhnosti ispol'zovaniia antibiotikov v lechenii bol'nykh ostrym kholetsistitom.

The authors studied the efficacy of directed transport of antibiotics in autologous blood shadows in the complex treatment of 73 patients with acute cholecystitis (the main group) in comparison with the traditional nonoperative treatment of 67 patients with the same disease (the control group). The directed transport of antibiotics in autologous blood shadows makes it possible to arrest adequately the clinico-laboratory manifestations of acute cholecystitis, thus preventing the development of infectious complications of the disease, shorten by 4.5 times the terms for arresting the clinico-laboratory manifestations of acute cholecystitis, and to optimize planned operative treatment. The suggested method increases the quality and the economy of management of surgical patients.

[Means of preventing suppurative complications following surgery of the liver]. / Puti profilaktiki gnoinykh oslozhnenii posle operatsii na pecheni.

The article analyses the results of antibiotic prevention of postoperative infectious complications and the use of special techniques and operative manipulations in 60 patients with localized affections of the liver. It was found that the most effective method of antibiotic prevention of complications is a purposeful transport of antibiotics in autologous erythrocytic shadows, which are infused intravenously once before the operation and, in extensive resection of the liver, on the second or third postoperative day. With the use of special techniques and operative manipulations in combination with purposeful transport of antibiotics the frequency of infectious complications reduces and the results of treatment in surgery of localized affections of the liver improve.

[Dynamics of glutathione metabolizing enzyme activity in experimental acute cholecystitis]. / Dinamika aktivnosti fermentov obmena glutationa pri éksperimental'nom ostrom kholetsistite.

Dynamics of glutathione-related enzymes activity was studied in erythrocytes of 22 dogs with destructive form of cholecystitis. As clinical symptoms of intoxication developed the enzymatic activity was decreased. In the animals with purulent-inflammatory complications distinct decrease was detected in activity of glutathione reductase (by 54.3%), glutathione-S-transferase--by 46.94% and glutathione peroxidase--by 42.1% (P less than 0.05). These data suggest that specific methods should be chosen for correction of impairments in the enzymatic activity in order to improve the treatment course efficiency as well as for prophylaxis of complications. The procedure developed for cholecystitis treatment, which involved channelled transport of antibiotics by means of autologous erythrocyte ghosts, proved to be more effective as compared with routine methods as shown by evaluation of the animals clinical state as well as by dynamics of hepato-specific enzymes activity and the glutathione-related enzymes activity. This procedure may be used in clinical practice; the laboratory tests described may serve for evaluation of the treatment course efficiency.

[Comprehensive therapy of acute cholecystitis in elderly and very old patients]. / Komplekcnoe lechenie ostrogo kholetsistita u bol'nykh pozhilogo i starcheskogo vozrasta.

A high effectiveness of the use of target transport of antibiotics in the autological erythrocytic shadows in the complex of surgical treatment of acute cholecystitis in 65 elderly and senile patients when compared with that in use of the existing methods of conservative treatment was revealed. The method developed permits to create in the bile a high and prolonged antibiotic concentration, and as well to reduce 4.5-fold the time for cupping off acute cholecystitis, to increase the quality of examination, preoperative preparation and elective operative treatment of the patients.

[Pharmacokinetics of kanamycin during targeted delivery to the liver in erythrocyte ghosts in animals with experimental acute cholecystitis]. / Farmakokinetika kanamitsina pri napravlennom transporte v pechen' v teniakh éritrotsitov u zhivotnykh s éksperimental'nym ostrym kholetsistitom.

Pharmacokinetics of kanamycin was studied after its targeted delivery to the liver in autological erythrocyte ghosts on 25 noninbred dogs with experimental acute cholecystitis in comparison to the routine intravenous administration of the antibiotic in solution. Kanamycin concentrations in the tissues of the liver, pancreas, spleen, kidneys and lungs as well as in bile and blood serum were determined by the agar diffusion method 24, 48 and 72 hours after the last administration. It was found that the targeted delivery of kanamycin in blood shadows made it possible to provide high concentrations of the antibiotic for prolonged periods in the liver and biliary ducts and to more efficiently arrest the clinical manifestations of acute cholecystitis as well as normalize the laboratory indices. The data showed that using blood shadows as a reliable system for targeted delivery of antibiotics to the liver was advisable in purulent inflammatory affections of the biliary ducts.

[Features of incorporation of several antibiotics into erythrocyte ghosts--a system of targeted delivery of chemotherapeutic drugs]. / Osobennosti vkliucheniia nekotorykh antibiotikov v éritrotsitarnye teni--sistemu tselenaproavlennoi dostavki khimioterapevticheskikh preparatov.

Features of incorporation of cefazolin, ampicillin, kanamycin and gentamicin into erythrocytic shadows in patients with abdominal diseases and apparently healthy persons during hypoosmotic hemolysis were studied. The highest percentage of kanamycin and gentamicin incorporation into the erythrocytic shadows was observed. The parameter of the antibiotic incorporation from the incubation fluid into the erythrocytic shadows depended on the antibiotic type and dose. Rather significant resistance of the erythrocytic carriers with incorporated gentamicin to possible desorption and excretion of the antibiotic from the shadows into the blood flow was revealed which made their use possible for target release of antibiotics in the liver.

[Experimental bases of directed transport of antibiotics in suppurative-inflammatory diseases of the liver and biliary tract]. / Eksperimental'noe obosnovanie napravlennogo transporta antibiotikov pri gnoino-vospalitel'nykh zabolevaniiakh pecheni i zhelchnykh putei.

The possibility of use and effectiveness of directed transport of antibiotics in erythrocyte carriers in the treatment of suppurative-inflammatory diseases of biliary ducts has been studied on dogs. It has been found that erythrocyte carriers were resistant to desorption of antibiotics in the blood circulation. Pharmacokinetic investigation has shown the more prolonged collection of antibiotics in hepatic tissue in comparison with traditional intravenous route. The results of clinical, pharmacokinetic and morphological investigation have shown more effectiveness of directed transport of antibiotics to the liver in autologous erythrocyte carriers in treatment of suppurative diseases of biliary ducts in comparison with traditional methods of infusion of antibiotics. For reasons given it is recommended to use this method in clinical practice.

[Clinico-immunologic status and postoperative course of patients with chronic pancreatitis and complicated cholecystitis]. / Kliniko-immunologicheskii status i techenie posleoperatsionnogo perioda u bol'nykh khronicheskim pankreatitom i oslozhnennym kholetsistitom.

Patients with chronic pancreatitis and complicated calculous cholecystitis are known to have considerable disturbances of the immune status of the organism. The complex of preoperative management and postoperative treatment of such patients should include drugs mainly influencing the cell immunity indices.