[Nature of the IR spectra of intact cells of Gram-negative microorganisms]. / K voposu o prirode IK-spektrov intaktnykh kletok nekotorykh gramotritsatel'nykh mikroorganizmov.

The character of changes in the infrared spectra of E. coli in the process of their mechanical disintegration has been studied. The destruction of E. coli cell structures has been shown to produce no changes in the optical density of the main analytical absorption bands in infrared spectra. This fact suggests that the infrared absorption spectra of E. coli are the sum of the spectra of all chemical components of the cell, which is confirmed by the infrared spectral study of E. coli cell-wall preparations. Similar results have been obtained in the study of the preparations of B. pertussis cell walls, protoplasts and intact cells.

[Potential use of infrared spectroscopy for the quantitative characterization of the chemical composition of Salmonella typhi preparations]. / Vozmozhnost' primeneniia infrakrasnoi spektroskopii dlia kolichestvennoi kharakteristiki khimicheskogo sostava preparatov Salmonella typhi.

The possibility of using infrared spectroscopy for the evaluation of the chemical composition of S. typhi preparations is shown. The existence of a clear-cut correlation between the optical density of adsorption bands in the region between 1,665 and 1,100 cm-1 and the content of protein, polysaccharide and nucleic acids in the preparations subjected to analysis has been revealed. The character of changes in the infrared spectra of the preparations at different stages of deep cultivation has been studied.

[Application of infrared spectroscopy to enterobacteria research]. / Primenenie infrakrasnoi spektroskopii v issledovanii énterobakterii.

The influence of technology used in the preparation of spectroscopic specimens on the intensity of analytical adsorption bands in the infrared spectra of intact microbial cells has been studied with the use of mathematical planning methods. The optimal technology of specimen preparation by KBr immersion has been selected. This technology ensures the measuring of the relative optical density of the main analytical adsorption bands with an error not exceeding 3%. As an example, several E. coli strains have been used to demonstrate the possibility of their differentiation by the infrared adsorption spectra of intact cells.