Subject(s)
Actinomycetales Infections/diagnosis , Arcanobacterium/isolation & purification , Brain Abscess/diagnosis , Brain Neoplasms/diagnosis , Actinomycetales Infections/drug therapy , Amoxicillin/administration & dosage , Amoxicillin/therapeutic use , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/therapeutic use , Biopsy , Brain Abscess/drug therapy , Brain Abscess/microbiology , Brain Abscess/pathology , Diagnosis, Differential , Drug Therapy, Combination , Humans , Male , Metronidazole/administration & dosage , Metronidazole/therapeutic use , Middle Aged , Mouth/microbiology , Oral Hygiene , Paresis/etiology , Stereotaxic TechniquesABSTRACT
This study investigated meiotic segregation in spermatozoa to determine if severe teratozoospermia should prevent the use of intracytoplasmic sperm injection (ICSI) because of the high production of gametes with chromosomal aneuploidies and analysed DNA fragmentation in gametes from the same semen to determine if DNA integrity was worse in patients with severe teratozoospermia. Sperm samples from 12 infertile patients were studied by fluorescence in-situ hybridization for chromosomes X, Y, 13, 18 and 21 and by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling. Four patients with a majority of macrocephalic forms with multiple flagella had more than 99% spermatozoa with abnormal chromosomal content. The other patients (globozoospermia or other abnormalities concerning sperm heads) had no increased aneuploidy or a slightly significant increase (P<0.05). The rate of DNA fragmentation was significantly higher in infertile patients than in the controls (P<0.001; 14.3% versus 1.20%, respectively) but presented important variability. Therefore, ICSI should not be attempted if men have macrocephalic gametes with multiple flagella but morphology is not always a good predictor of chromosomal content, depending upon the kind of teratozoospermia. Evaluation of the rate of aneuploidy and DNA fragmentation in gametes of patients with severe teratozoospermia is recommended.
Subject(s)
Aneuploidy , DNA Fragmentation , Infertility, Male/genetics , Spermatozoa/physiology , Humans , In Situ Hybridization, Fluorescence , In Situ Nick-End Labeling , Male , Semen Analysis , Spermatozoa/abnormalities , Spermatozoa/cytologyABSTRACT
OBJECTIVE: In this study we report the prevalence of the throat infections to Streptococcus pyogenes in child and adult in Morocco, and the current antimicrobial susceptibility of the aminopenicillins and erythromycin. PATIENTS AND METHODS: A prospective surveillance study was made from March 2006 to February 2007 in four primary health care in Rabat and Sale cities. Six hundred and ninety-seven patients (494 children and 203 adults) were the object of samplings by throat cotton swab probe, for researching the group A Streptococcus (GAS). The patients were those proposed by their doctors for the treatment of throat infections. The antimicrobial susceptibility was realized by two methods: the disc-diffusion method on Mueller-Hinton agar plates and MICs were determined by E-test. The antibiotics tested were the penicillin G, the amoxicillin and the erythromycin. RESULTS: S. pyogenes was identified in 65 cases (9.3%): 45 strains in children (9.1%) and 20 strains in adults (9.9%). Two peaks of throat infections to S. pyogenes were recorded in children aged 12 to 15 years and in adults between 36 and 39 years old. All strains were susceptible to penicillin G and amoxicillin. One strain was resistant to erythromycin with a MIC greater than 256 microg/mL.
