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1.
Nat Commun ; 13(1): 540, 2022 01 27.
Article in English | MEDLINE | ID: mdl-35087057

ABSTRACT

G protein-coupled receptors (GPCRs) activate G proteins and undergo a complex regulation by interaction with GPCR kinases (GRKs) and the formation of receptor-arrestin complexes. However, the impact of individual GRKs on arrestin binding is not clear. We report the creation of eleven combinatorial HEK293 knockout cell clones lacking GRK2/3/5/6, including single, double, triple and the quadruple GRK knockout. Analysis of ß-arrestin1/2 interactions for twelve GPCRs in our GRK knockout cells enables the differentiation of two main receptor subsets: GRK2/3-regulated and GRK2/3/5/6-regulated receptors. Furthermore, we identify GPCRs that interact with ß-arrestins via the overexpression of specific GRKs even in the absence of agonists. Finally, using GRK knockout cells, PKC inhibitors and ß-arrestin mutants, we present evidence for differential receptor-ß-arrestin1/2 complex configurations mediated by selective engagement of kinases. We anticipate our GRK knockout platform to facilitate the elucidation of previously unappreciated details of GRK-specific GPCR regulation and ß-arrestin complex formation.


Subject(s)
Arrestin/metabolism , G-Protein-Coupled Receptor Kinases/metabolism , Receptors, G-Protein-Coupled/metabolism , GTP-Binding Proteins/metabolism , HEK293 Cells , Humans , Phosphorylation , Signal Transduction/physiology , beta-Arrestin 1/metabolism , beta-Arrestin 2/metabolism
2.
Inorg Chem ; 59(14): 9951-9961, 2020 Jul 20.
Article in English | MEDLINE | ID: mdl-32614570

ABSTRACT

The neutral, homoleptic pyridylphosphininenickel(0) complex [Ni(2-Py-4,6-Ph2-PC5H2)2] (1) has been obtained by reaction of the formal Ni(0) source [(IPr)Ni(H2C═CHSiMe3)2] with 2 equiv of 2-(2'-pyridyl)-4,6-diphenylphosphinine (L). Compound 1 can be oxidized both electrochemically and through the use of ferrocenium salts, to afford the corresponding Ni(I) complexes [1]BF4, [1(THF)]PF6, and [12](BArF4)2. The structures of these salts reveal an interesting dependence on the nature of the anion. While [1]BF4 and [1(THF)]PF6 show trigonal-bipyramidal coordination of Ni in the solid state, [12](BArF4)2 exists as a dinuclear Ni(I) complex and possesses a bridging phosphinine moiety in a rare µ2 mode. Reactions of 1 with halobenzenes highlight the noninnocent behavior of the aromatic phosphinine ligand, leading to the formation of oxidized Ni complexes but not to classical oxidative addition products. The reaction of 1 with bromobenzene affords the λ5 phosphinine 2 and the bipyramidal Ni(I) complex [1]Br, whereas a more unconventional oxidation product 3 is formed from the reaction of 1 and iodobenzene.

3.
Eur Neuropsychopharmacol ; 36: 154-159, 2020 07.
Article in English | MEDLINE | ID: mdl-32522387

ABSTRACT

A polymorphism in the gene encoding the serotonin (5-HT) transporter (5-HTT) has been shown to moderate the response to CO2 inhalation, an experimental model for panic attacks (PAs). Recurrent, unpredictable PAs represent, together with anticipatory anxiety of recurring attacks, the core feature of panic disorder (PD) and significantly interfere with patients' daily life. In addition to genetic components, accumulating evidence suggests that epigenetic mechanisms, which regulate gene expression by modifying chromatin structure, also play a fundamental role in the etiology of mental disorders. However, in PD, epigenetic mechanisms have barely been examined to date. In the present study, we investigated the relationship between methylation at the regulatory region of the gene encoding the 5-HTT and the reactivity to a 35% CO2 inhalation in PD patients. We focused on four specific CpG sites and found a significant association between the methylation level of one of these CpG sites and the fear response. This suggests that the emotional response to CO2 inhalation might be moderated by an epigenetic mechanism, and underlines the implication of the 5-HT system in PAs. Future studies are needed to further investigate epigenetic alterations in PD and their functional consequences. These insights can increase our understanding of the underlying pathophysiology and support the development of new treatment strategies.


Subject(s)
Carbon Dioxide/adverse effects , DNA Methylation/physiology , Fear/physiology , Panic Disorder/metabolism , Regulatory Sequences, Nucleic Acid/physiology , Serotonin Plasma Membrane Transport Proteins/metabolism , Adult , Base Sequence , Epigenesis, Genetic/drug effects , Epigenesis, Genetic/physiology , Fear/drug effects , Fear/psychology , Female , Humans , Male , Middle Aged , Panic Disorder/genetics , Panic Disorder/psychology , Serotonin Plasma Membrane Transport Proteins/genetics
4.
J Organomet Chem ; 870: 121-129, 2018 Sep 01.
Article in English | MEDLINE | ID: mdl-31105336

ABSTRACT

This report presents the synthesis and characterization of mono- and bis(amino acid ester) ferrocene complexes generated using a sulfonamide linking strategy as an alternative to the more heavily explored amide linking strategy. These compounds were investigated to test their ability to form hydrogen bonding interactions both in the solid state and in solution, and were compared to the previously observed intramolecular interstrand crosslinking seen in amide-linked ferrocene constructs. Synthesized compounds also included controls that do not exhibit sulfonamide N-H bonds and thus cannot engage in hydrogen bonding. In the solid state, we observe both S=O⋯H-N and C=O⋯H-N intermolecular interactions, but we do not observe any intramolecular interstrand hydrogen bonding. In the solution phase, we also do not see any intramolecular hydrogen bonding interactions in these compounds as measured by titration of d6-DMSO as a competitive hydrogen bonding reagent. We also collected CD spectra on these compounds, which revealed that the chiral peptides can induce dichroism in the dd transition of the ferrocene units. Our results indicate that the peptide-ferrocene linking group governs whether intermolecular hydrogen bonding interactions can occur between the amino acids adjacent to the cyclopentadienyl groups.

5.
Transl Psychiatry ; 6: e773, 2016 Apr 05.
Article in English | MEDLINE | ID: mdl-27045843

ABSTRACT

Epigenetic signatures such as methylation of the monoamine oxidase A (MAOA) gene have been found to be altered in panic disorder (PD). Hypothesizing temporal plasticity of epigenetic processes as a mechanism of successful fear extinction, the present psychotherapy-epigenetic study for we believe the first time investigated MAOA methylation changes during the course of exposure-based cognitive behavioral therapy (CBT) in PD. MAOA methylation was compared between N=28 female Caucasian PD patients (discovery sample) and N=28 age- and sex-matched healthy controls via direct sequencing of sodium bisulfite-treated DNA extracted from blood cells. MAOA methylation was furthermore analyzed at baseline (T0) and after a 6-week CBT (T1) in the discovery sample parallelized by a waiting time in healthy controls, as well as in an independent sample of female PD patients (N=20). Patients exhibited lower MAOA methylation than healthy controls (P<0.001), and baseline PD severity correlated negatively with MAOA methylation (P=0.01). In the discovery sample, MAOA methylation increased up to the level of healthy controls along with CBT response (number of panic attacks; T0-T1: +3.37±2.17%), while non-responders further decreased in methylation (-2.00±1.28%; P=0.001). In the replication sample, increases in MAOA methylation correlated with agoraphobic symptom reduction after CBT (P=0.02-0.03). The present results support previous evidence for MAOA hypomethylation as a PD risk marker and suggest reversibility of MAOA hypomethylation as a potential epigenetic correlate of response to CBT. The emerging notion of epigenetic signatures as a mechanism of action of psychotherapeutic interventions may promote epigenetic patterns as biomarkers of lasting extinction effects.


Subject(s)
Cognitive Behavioral Therapy , DNA Methylation , Epigenesis, Genetic , Monoamine Oxidase/genetics , Panic Disorder/genetics , Adult , Case-Control Studies , Female , Humans , Panic Disorder/therapy , Sequence Analysis, DNA
6.
Resuscitation ; 101: 102-7, 2016 Apr.
Article in English | MEDLINE | ID: mdl-26875990

ABSTRACT

BACKGROUND/OBJECTIVE: Physiologic monitoring of resuscitative efforts during cardiac arrest is gaining in importance, as it provides a real-time window into the cellular physiology of patients. The aim of this review is to assess the quality of evidence surrounding the use of physiologic monitoring to guide cardiopulmonary resuscitation (CPR), and to examine whether the evidence demonstrates an improvement in patient outcome when comparing hemodynamic-directed CPR versus standard CPR. METHODS: Studies were obtained through a search of the PubMed, Embase and Cochrane databases. Peer-reviewed randomized trials, case-control studies, systematic reviews, and cohort studies that titrated CPR to physiologic measures, compared results to standard CPR, and examined patient outcome were included. RESULTS: Six studies met inclusion criteria, with all studies conducted in animal populations. Four studies examined the effects of hemodynamic-directed CPR on survival, with 35/37 (94.6%) animals surviving in the hemodynamic-directed CPR groups and 12/35 (34.3%) surviving in the control groups (p<0.001). Two studies examined the effects of hemodynamic-directed CPR on ROSC, with 22/30 (73.3%) achieving ROSC in the hemodynamic-directed CPR group and 19/30 (63.3%) achieving ROSC in the control group (p=0.344). DISCUSSION/CONCLUSION: These results suggest a trend in survival from hemodynamic-directed CPR over standard CPR, however the small sample size and lack of human data make these results of limited value. Future human studies examining hemodynamic-directed CPR versus current CPR standards are needed to enhance our understanding of how to effectively use physiologic measures to improve resuscitation efforts and ultimately incorporate concrete targets into international resuscitation guidelines.


Subject(s)
Cardiopulmonary Resuscitation/methods , Feedback, Physiological , Heart Arrest/therapy , Hemodynamics , Monitoring, Physiologic , Feedback , Humans
7.
Leukemia ; 30(5): 1116-25, 2016 05.
Article in English | MEDLINE | ID: mdl-26867669

ABSTRACT

Waldenström's macroglobulinemia (WM) is a B-cell non-Hodgkin's lymphoma (B-NHL) characterized by immunoglobulin M (IgM) monoclonal gammopathy and the medullary expansion of clonal lymphoplasmacytic cells. Neoplastic transformation has been partially attributed to hyperactive MYD88 signaling, secondary to the MYD88 L265P mutation, occurring in the majority of WM patients. Nevertheless, the presence of chronic active B-cell receptor (BCR) signaling, a feature of multiple IgM+ B-NHL, remains a subject of speculation in WM. Here, we interrogated the BCR signaling capacity of primary WM cells by utilizing multiparametric phosphoflow cytometry and found heightened basal phosphorylation of BCR-related signaling proteins, and augmented phosphoresponses on surface IgM (sIgM) crosslinking, compared with normal B cells. In support of those findings we observed high sIgM expression and loss of phosphatase activity in WM cells, which could both lead to signaling potentiation in clonal cells. Finally, led by the high-signaling heterogeneity among WM samples, we generated patient-specific phosphosignatures, which subclassified patients into a 'high' and a 'healthy-like' signaling group, with the second corresponding to patients with a more indolent clinical phenotype. These findings support the presence of chronic active BCR signaling in WM while providing a link between differential BCR signaling utilization and distinct clinical WM subgroups.


Subject(s)
B-Lymphocytes/pathology , Receptors, Antigen, B-Cell/physiology , Signal Transduction , Waldenstrom Macroglobulinemia/pathology , Clone Cells/pathology , Female , Humans , Immunoglobulin M/metabolism , Male , Phosphorylation
8.
Oper Orthop Traumatol ; 28(2): 111-26; quiz 127, 2016 Apr.
Article in German | MEDLINE | ID: mdl-26769008

ABSTRACT

OBJECTIVE: Reconstruction of the ruptured ulnar collateral ligament of the metacarpophalangeal (MP) joint of the thumb. INDICATIONS: Ruptured ulnar collateral ligament of the thumb MP joint with instability: joint opening of more than 30° in flexion and more than 20° in extension, Stener lesion, displaced avulsion fractures. CONTRAINDICATIONS: Abrasions, wound-healing disturbance, skin disease, osteoarthritis. SURGICAL TECHNIQUE: Curved skin incision dorsoulnar above the thumb MP joint. Protection of the branches of the superficial radial nerve. Incision of the adductor aponeurosis. Exposing the ulnar collateral ligament; opening and examination of the joint. Depending on the injury, primary suture repair, transosseous suture, repair with a bone anchor, osteosynthesis with K-wires or small screws in avulsion fracture, ligament reconstruction in chronic instability or older injury. POSTOPERATIVE TREATMENT: Cast splint of the MP joint until swelling subsides; cast immobilization for 6 weeks; range-of-motion exercises, avoiding forced radial deviation of the MP joint for 3 months. RESULTS: Complete joint stability 3 months postoperatively in all 34 patients with rupture of the ulnar collateral ligament.


Subject(s)
Collateral Ligament, Ulnar/injuries , Collateral Ligament, Ulnar/surgery , Metacarpophalangeal Joint/injuries , Metacarpophalangeal Joint/surgery , Thumb/injuries , Ulnar Collateral Ligament Reconstruction/methods , Adult , Arthroplasty/instrumentation , Arthroplasty/methods , Female , Humans , Male , Middle Aged , Recovery of Function , Thumb/surgery , Treatment Outcome , Ulnar Collateral Ligament Reconstruction/instrumentation
9.
World J Biol Psychiatry ; 17(1): 76-83, 2016.
Article in English | MEDLINE | ID: mdl-26488131

ABSTRACT

OBJECTIVES: Social anxiety has been suggested to be promoted by an insecure attachment style. Oxytocin is discussed as a mediator of trust and social bonding as well as a modulator of social anxiety. Applying a gene-environment (G × E) interaction approach, in the present pilot study the main and interactive effects of attachment styles and oxytocin receptor (OXTR) gene variation were probed in a combined risk factor model of social anxiety in healthy probands. METHODS: Participants (N = 388; 219 females, 169 males; age 24.7 ± 4.7 years) were assessed for anxiety in social situations (Social Phobia and Anxiety Inventory) depending on attachment style (Adult Attachment Scale, AAS) and OXTR rs53576 A/G genotype. RESULTS: A less secure attachment style was significantly associated with higher social anxiety. This association was partly modulated by OXTR genotype, with a stronger negative influence of a less secure attachment style on social anxiety in A allele carriers as compared to GG homozygotes. CONCLUSIONS: The present pilot data point to a strong association of less secure attachment and social anxiety as well as to a gene-environment interaction effect of OXTR rs53576 genotype and attachment style on social anxiety possibly constituting a targetable combined risk marker of social anxiety disorder.


Subject(s)
Anxiety/diagnosis , Anxiety/genetics , Gene-Environment Interaction , Genetic Variation , Object Attachment , Receptors, Oxytocin/genetics , Adult , Female , Genotype , Germany , Healthy Volunteers , Humans , Male , Pilot Projects , Psychiatric Status Rating Scales , Regression Analysis , Self Report , Young Adult
10.
Dalton Trans ; 44(39): 17268-77, 2015 Oct 21.
Article in English | MEDLINE | ID: mdl-26374670

ABSTRACT

Dimeric metal complexes can often exhibit coupling interactions via bridging ligands. In this report, we present two Re(CO)3 dimers, where the metals are linked via a bis(pyca) hydrazine (pyca = pyridine-2-carbaldehyde imine) Schiff base ligand. For the dimeric compounds 4 and 5, we observe strong coupling across the dimer as measured by cyclic voltammetry: ∼480 mV separations between the first and the second reduction waves that correspond to comproportionation constants close to 1.5 × 10(8). Evidence for a mixed valence state upon one electron reduction was also observed by spectroelectrochemistry in which a clear inter-valence charge-transfer (IVCT) band was observed in [4]- and [5]-complexes. The electronic structures of all target compounds were probed by DFT and TDDFT computational methods. DFT calculations indicate that reduction takes place at the diimine units, and that the observed coupling is a ligand-based phenomenon, rather than one that involves metal-based orbitals.


Subject(s)
Carbon/chemistry , Coordination Complexes/chemistry , Hydrazines/chemistry , Oxygen/chemistry , Ruthenium/chemistry , Carbon/metabolism , Coordination Complexes/metabolism , Dimerization , Hydrazines/metabolism , Oxygen/metabolism , Ruthenium/metabolism , X-Ray Diffraction
11.
Dalton Trans ; 44(35): 15400-3, 2015 Sep 21.
Article in English | MEDLINE | ID: mdl-26252161

ABSTRACT

Re(CO)3 conjugates 1 and 2 that incorporate azobenzenes can be readily generated via one-pot reactions using Schiff base reaction forming conditions. Excitation of the MLCT bands in 1 and 2 results in isomerization of the azobenzene moiety, and this process has been investigated via time-resolved photophysics and TDDFT calculations.

12.
Pharmacopsychiatry ; 48(4-5): 145-9, 2015 Jul.
Article in English | MEDLINE | ID: mdl-26011568

ABSTRACT

INTRODUCTION: Disturbances of circadian rhythms occur in all episodes of bipolar disorder (BD). Lithium, as gold-standard in the maintenance treatment of BD, is known to influence circadian processes. METHODS: In a pilot study lymphoblastoid cell lines (LCLs) were generated from 8 BD patients and 6 healthy controls. The LCLs were treated with lithiumchloride (LiCl) for 3 weeks. Cell cycles were then synchronized and expressional analysis by quantitative Real Time PCR was done. RESULTS: BD and controls differed in the period length regarding DBP (albumin D-box binding protein) expression and DBP expression was also influenced by lithium treatment. Furthermore, baseline DBP expression was significantly different between non-treated BD and healthy controls. None of the other analyzed circadian genes showed to be influenced by chronic lithium treatment or to be differentially regulated due to the diagnosis. DISCUSSION: We here show that chronic lithium treatment of LCLs leads to decreased expression of the clock gene DBP, rendering DBP a lithium-regulated gene. We could confirm the role of the circadian clock as well in lithium mode of action as in the pathomechanisms of BD although future studies with a greater number of participants and cell lines are needed.


Subject(s)
Antimanic Agents/pharmacology , Bipolar Disorder/drug therapy , Circadian Clocks , DNA-Binding Proteins/biosynthesis , Gene Expression/drug effects , Lithium Chloride/pharmacology , Transcription Factors/biosynthesis , Cell Line , Female , Humans , Male , Pilot Projects
13.
Phys Chem Chem Phys ; 17(4): 2349-51, 2015 Jan 28.
Article in English | MEDLINE | ID: mdl-25500795

ABSTRACT

The first ultrafast study of the dimeric fluorescent BF2 dye BOPHY is presented. When compared to a structurally related BODIPY dye, similar photophysical dynamics are observed, including an intermediate kinetic component present in both dye types.

14.
J Chem Phys ; 139(4): 044703, 2013 Jul 28.
Article in English | MEDLINE | ID: mdl-23901999

ABSTRACT

The interaction between zinc-tetraphenylporphyrin (ZnTPP) and fullerenes (C60 and C60F48) are studied using ultraviolet photoelectron spectroscopy (UPS) and scanning tunneling microscopy (STM). Low temperature STM reveals highly ordered ZnTPP monolayers on Au(111). In contrast to C60, a submonolayer coverage of C60F48 results in long-range disorder of the underlying single ZnTPP layer and distortion of individual ZnTPP molecules. This is induced by substantial charge transfer at the organic-organic interface, revealed by the interface energetics from UPS. However, a second layer of ZnTPP prevents C60F48 guests from breaking the self-assembled porphyrin template. This finding is important for understanding the growth behaviour of "bottom-up" functional nanostructures involving strong donor-acceptor heterojunctions in molecular electronics.

15.
Nat Commun ; 4: 2137, 2013.
Article in English | MEDLINE | ID: mdl-23842527

ABSTRACT

The mechanosensitive channel of small conductance (MscS) has been characterized at both functional and structural levels and has an integral role in the protection of bacterial cells against hypoosmotic shock. Here we investigate the role that the cytoplasmic domain has in MscS channel function by recording wild-type and mutant MscS single-channel activity in liposome patches. We report that MscS preferentially resides in subconducting states at hyperpolarising potentials when Ca(2+) and Ba(2+) ions are the major permeant cations. In addition, our results indicate that charged residues proximal to the seven vestibular portals and their electrostatic interactions with permeating cations determine selectivity and regulate the conductance of MscS and potentially other channels belonging to the MscS subfamily. Furthermore, our findings suggest a role for mechanosensitive channels in bacterial calcium regulation, indicative of functions other than protection against osmolarity changes that these channels possibly fulfil in bacteria.


Subject(s)
Calcium/metabolism , Escherichia coli Proteins/chemistry , Escherichia coli/chemistry , Ion Channels/chemistry , Mechanotransduction, Cellular/physiology , Membrane Potentials/physiology , Barium/chemistry , Barium/metabolism , Calcium/chemistry , Cations, Divalent , Databases, Protein , Escherichia coli/genetics , Escherichia coli/metabolism , Escherichia coli Proteins/genetics , Escherichia coli Proteins/metabolism , Ion Channel Gating , Ion Channels/genetics , Ion Channels/metabolism , Ion Transport , Liposomes/chemistry , Models, Molecular , Mutation , Protein Structure, Tertiary , Static Electricity , Thermoanaerobacter/chemistry , Thermoanaerobacter/metabolism
16.
Rev Med Suisse ; 9(369): 140-1, 2013 Jan 16.
Article in French | MEDLINE | ID: mdl-23409655

ABSTRACT

Some paediatrics centres routinely send the medical consultation letter not only to the primary or referring physician but to the patient and his/her family as well. This way of sharing medical information is appreciated not only by the patients themselves but also by a majority of physicians.


Subject(s)
Documentation , Patient Participation , Referral and Consultation , Child , Humans
17.
Mol Cell Endocrinol ; 371(1-2): 189-94, 2013 May 22.
Article in English | MEDLINE | ID: mdl-23267837

ABSTRACT

Pheochromocytoma is a rare but potentially lethal chromaffin cell tumor with currently no effective treatment. Peptide hormone receptors are frequently overexpressed on endocrine tumor cells and can be specifically targeted by various anti-tumor peptide analogs. The present study carried out on mouse pheochromocytoma cells (MPCs) and a more aggressive mouse tumor tissue-derived (MTT) cell line revealed that these cells are characterized by pronounced expression of the somatostatin receptor 2 (sst2), growth hormone-releasing hormone (GHRH) receptor and the luteinizing hormone-releasing hormone (LHRH) receptor. We further demonstrated significant anti-tumor effects mediated by cytotoxic somatostatin analogs, AN-162 and AN-238, by LHRH antagonist, Cetrorelix, by the cytotoxic LHRH analog, AN-152, and by recently developed GHRH antagonist, MIA-602, on MPC and for AN-152 and MIA-602 on MTT cells. Studies of novel anti-tumor compounds on these mouse cell lines serve as an important basis for mouse models of metastatic pheochromocytoma, which we are currently establishing.


Subject(s)
Adrenal Gland Neoplasms/drug therapy , Pheochromocytoma/drug therapy , Receptors, Neuropeptide/drug effects , 2-Hydroxyphenethylamine/analogs & derivatives , 2-Hydroxyphenethylamine/pharmacology , Aniline Compounds/pharmacology , Animals , Apoptosis/drug effects , Cell Line, Tumor , Cell Proliferation/drug effects , Cell Survival/drug effects , Doxorubicin/analogs & derivatives , Doxorubicin/pharmacology , Gonadotropin-Releasing Hormone/analogs & derivatives , Gonadotropin-Releasing Hormone/antagonists & inhibitors , Gonadotropin-Releasing Hormone/pharmacology , Growth Hormone-Releasing Hormone/antagonists & inhibitors , Mice , Pyrroles/pharmacology , Receptors, LHRH/biosynthesis , Receptors, LHRH/drug effects , Receptors, LHRH/metabolism , Receptors, Neuropeptide/biosynthesis , Receptors, Neuropeptide/metabolism , Receptors, Pituitary Hormone-Regulating Hormone/biosynthesis , Receptors, Pituitary Hormone-Regulating Hormone/drug effects , Receptors, Pituitary Hormone-Regulating Hormone/metabolism , Receptors, Somatostatin/biosynthesis , Receptors, Somatostatin/drug effects , Receptors, Somatostatin/metabolism , Sermorelin/analogs & derivatives , Sermorelin/pharmacology , Somatostatin/analogs & derivatives
18.
Support Care Cancer ; 21(5): 1383-93, 2013 May.
Article in English | MEDLINE | ID: mdl-23238655

ABSTRACT

PURPOSE: Compared to the literature on other malignancies, data on quality of life (QoL) in bladder cancer are sparse. This study sought answers to the following questions: In what QoL domains do patients with bladder cancer differ from the general population? Do patients with radical cystectomy differ in QoL compared to those who received conservative treatment? Do patients with neobladder generally have better QoL compared to patients with other diversion methods? METHODS: At the beginning of inpatient rehabilitation, N = 823 patients with bladder cancer were assessed. Data of a representative community sample (N = 2037) were used for comparison. The questionnaire EORTC QLQ-C30 was used to measure QoL. Multivariate linear regression models were computed to investigate differences between groups. RESULTS: Patients with both non-muscle invasive and muscle invasive bladder cancer reported significantly more problems and worse functioning than the general population. Radiotherapy is associated with clinically relevant more pain, dyspnoea, constipation, appetite loss and decreased social functioning while chemotherapy is associated more with dyspnoea. Cystectomy patients reported more fatigue, appetite loss and decreased role functioning. Male patients ≥70 years with conduit experienced more sleep and emotional problems. These effects of urinary diversion were not observed in women and younger patients. CONCLUSIONS: Patients with bladder cancer experience various QoL concerns at the beginning of inpatient rehabilitation. These problems can partly be explained by the type of treatment the patients receive. Type of urinary diversion is relevant for QoL in subgroups of patients.


Subject(s)
Cystectomy/methods , Quality of Life , Urinary Bladder Neoplasms/pathology , Urinary Diversion/methods , Adult , Age Factors , Aged , Aged, 80 and over , Antineoplastic Agents/adverse effects , Antineoplastic Agents/therapeutic use , Cross-Sectional Studies , Female , Humans , Linear Models , Male , Middle Aged , Multivariate Analysis , Neoplasm Invasiveness , Radiation Injuries/epidemiology , Sex Factors , Surveys and Questionnaires , Urinary Bladder Neoplasms/rehabilitation , Urinary Bladder Neoplasms/therapy , Young Adult
19.
Radiologe ; 51(10): 876-9, 2011 Oct.
Article in German | MEDLINE | ID: mdl-21845481

ABSTRACT

Several extensive surgical interventions of a cystic lesion in the left mandible were followed by recurrences. The lesion extended from the primary mandibular region into the area of pterygopalatine fossa finally infiltrating the orbital region and the skull base. Histological results could never demonstrate a malignancy with certainty. Due to the patient's poor general condition, the refusal for further surgical inventions and due to the malignoma-like growth pattern radiation treatment was performed. However, this had no effect on tumor progression.


Subject(s)
Mandibular Neoplasms/radiotherapy , Mandibular Neoplasms/surgery , Neoplasm Recurrence, Local/radiotherapy , Neoplasm Recurrence, Local/surgery , Odontogenic Tumor, Squamous/radiotherapy , Odontogenic Tumor, Squamous/surgery , Aged , Cutaneous Fistula/surgery , Disease Progression , Dose Fractionation, Radiation , Humans , Magnetic Resonance Imaging , Male , Mandible/surgery , Mandibular Neoplasms/pathology , Mandibular Prosthesis Implantation , Neoplasm Invasiveness/pathology , Neoplasm Recurrence, Local/pathology , Odontogenic Tumor, Squamous/pathology , Oral Fistula/surgery , Postoperative Complications/surgery , Radiography, Panoramic , Radiotherapy Planning, Computer-Assisted , Radiotherapy, Adjuvant , Radiotherapy, Conformal , Reoperation , Tomography, X-Ray Computed
20.
J Nanosci Nanotechnol ; 11(4): 3464-9, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21776724

ABSTRACT

A combined method of precipitation, phase transfer into organic solvent, solvothermal treatment and subsequent in situ polymerization was used to integrate nanocrystalline Bi2MoO6- and Bi2WO6-particles into a polymer matrix of poly-laurylacrylate. The presented method offers a new and gentle way to produce highly transparent bulk nanocomposites containing evenly distributed Bi2MoO6- and Bi2WO6-nanoparticles. Characterization results of DLS-, XRD-, REM- and TEM-measurements are presented as well as solid state UV/VIS-measurements of the particles. The transparent nanocomposites were characterized using UV/VIS-spectroscopy and ellipsometry. All composites show a good transmission in the range from 800-400 nm. The particle content of the nanocomposites was measured with TG-measurements.


Subject(s)
Bismuth/chemistry , Nanostructures/chemistry , Nanostructures/ultrastructure , Nanotechnology/methods , Polymers/chemistry , Macromolecular Substances/chemistry , Materials Testing , Molecular Conformation , Particle Size , Refractometry , Surface Properties
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