ABSTRACT
Urinary trypsin inhibitor (UTI, Ulinastatin) is a protease inhibitor that has not been yet used in Europe in any experimental trial of severe acute pancreatitis. We have combined the experimental model of severe, hemorrhagic form of acute pancreatitis, and pharmacological treatment with a protease inhibitor. Male Wistar rats were divided into four experimental groups: healthy controls, operated, operated with experimentally induced acute pancreatitis, and animals with acute pancreatitis--treated with UTI preparations. Subjects in the last group were administered UTI intraperitoneally 1 h after pancreatitis induction in an average standard dose of 3000 units/animal. Additionally, four subgroups were created in this treated group, based on the UTI administration time--number of standard doses received: 2 h - 1 standard dose, 6 h - 5 standard doses, 12 h - 11 doses, 24 and 48 h - 15 doses. Statistically significant differences in the serum amylase and lipase activity between the UTI-treated and non-treated subjects were found. In the group of non-treated animals, there a profound destruction of cellular organelles was observed with a total degradation of nuceli, endoplasmatic reticulum and zymogen granules. However, in the UTI-treated subjects, pathological processes proceeded with the significantly slower pace and in much smaller quantities.
Subject(s)
Glycoproteins/therapeutic use , Pancreas/ultrastructure , Pancreatitis, Acute Necrotizing/drug therapy , Trypsin Inhibitors/therapeutic use , Amylases/blood , Amylases/drug effects , Amylases/metabolism , Animals , Disease Models, Animal , Lipase/blood , Lipase/drug effects , Lipase/metabolism , Male , Pancreas/drug effects , Pancreas/pathology , Pancreatitis, Acute Necrotizing/pathology , Rats , Rats, WistarABSTRACT
The paper discusses three cases of mass bleeding from the upper section of gastrointestinal tract caused by benign tumours od the small bowel. The technique of the intraoperative endoscopic examination of gastrointestinal tract has been described. The authors stress the usefulness of this technique in the case when the source of bleeding is difficult to localise.
Subject(s)
Gastrointestinal Hemorrhage/etiology , Intestinal Neoplasms/complications , Intestinal Neoplasms/diagnosis , Aged , Female , Humans , Intestinal Neoplasms/surgery , Intestine, Small , Laparotomy , Male , Middle AgedABSTRACT
Communications show that in squamous cell carcinoma of the oesophagus one can often observe overexpression of p53 protein. Wild type p53 protein suppresses cells' growth, and mutated p53 acts as onkogene. Gene p53 mutations usually lead to stabilization and accumulation of p53 protein, and, as a result they become detectable by immunochemical methods. Overexpression of p53 has been observed in 28 (46.7%) out of 60 collected specimens. It has no connection with sex, age, histological differentiation, category (pT) of tumour, infiltration (pN) of lymph nodes and extent of metastases (pMlym+). p53 protein expression has no correlation with survival predictability.
