ABSTRACT
The synthesis of ZnInS (ZIS) quantum dots (QDs) in aqueous medium using thioglycolic acid (TGA) and sodium citrate as dual capping agents has been reported. The as-synthesized ZIS QDs were water soluble, emitting at 512 nm and nearly spherical in shape with average particle size of 8.9 ± 1.4 nm. The as-synthesized ZIS QDs were tested for its fluorescence response against different metal ions and the results revealed that ZIS QDs were selectively quenched by Co2+ions compared to other ions. The fluorescence sensing experiment showed that ZIS QDs has a linear response against the concentration of Co2+ions (0.1-100µM ) with the detection limit of 0.099µM. Based on the transmission electron microscope and absorption spectroscopy analyzes, the fluorescence quenching is attributed to the formation of surface ligand-metal complex (TGA-Co2+ions) which caused aggregation of the QDs. The present method explores the synthesis of zero-dimentional ZIS QDs and its potential in the selective detection of Co2+ions in aqueous solution.
ABSTRACT
The toxicity of heavy metals present in binary semiconductor nanoparticles also known as quantum dots (QDs) has hindered their wide applications hence the advent of non-toxic ternary quantum dots. These new group of quantum dots have been shown to possess some therapeutic action against cancer cell lines but not significant enough to be referred to as an ideal therapeutic agent. In this report, we address this problem by conjugating red emitting CuInS/ZnS QDs to a 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin -photosensitizer for improved bioactivities. The glutathione capped CuInS/ZnS QDs were synthesized in an aqueous medium using a kitchen pressure cooker at different Cu: In ratios (1:4 and 1:8) and at varied temperatures (95 °C, 190 °C and 235 °C). Optical properties show that the as-synthesized CuInS/ZnS QDs become red-shifted compared to the core (CuInS) after passivation with emission in the red region while the cytotoxicity study revealed excellent cell viability against normal kidney fibroblasts (BHK21). The highly fluorescent, water-soluble QDs were conjugated to 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP) via esterification reactions at room temperature. The resultant water-soluble conjugate was then used for the cytotoxicity, fluorescent imaging and gene expression study against human monocytic leukemia cells (THP-1). Our result showed that the conjugate possessed high cytotoxicity against THP-1 cells with enhanced localized cell uptake compared to the bare QDs. In addition, the gene expression study revealed that the conjugate induced inflammation compared to the QDs as NFKB gene was over-expressed upon cell inflammation while the singlet oxygen (1O2) study showed the conjugate possessed large amount of 1O2, three times than the bare porphyrin. Thus, the as-synthesized conjugate looks promising as a therapeutic agent for cancer therapy.
