ABSTRACT
We studied the clastogenic action of cyclophosphamide (CP) on bone marrow cells of the Armenian hamster (AH), Cricetulus migratorius. CP induced a dose-dependent linear increase in aberrant cells. The maximal cytogenetic action was observed 12 h after CP treatment. Male and female AHs were similarly sensitive to the clastogenic action of CP. We compared CP clastogenicity at a dose of 25 mg/kg on bone marrow cells of AHs, mice, rats, guinea pigs and Chinese hamsters 24 h after treatment. We observed that this dose of CP induced only 2.8% aberrant cells in bone marrow of AHs, but 42.8%, 32.2%, 25% and 14.6% aberrant cells in bone marrow of guinea pigs, rats, mice and Chinese hamsters respectively. AHs are much more resistant to the metaphase-arresting action of colchicine than other species of rodents (e.g., the colchicine dose for AHs is 100-fold more than for rats). Thus AHs are the most resistant of all rodent species studied to the clastogenic action of CP.
Subject(s)
Bone Marrow/drug effects , Chromosome Aberrations/genetics , Colchicine/toxicity , Mutagenesis , Animals , Bone Marrow Cells , Chromatids/drug effects , Cricetinae , Cricetulus , Dose-Response Relationship, Drug , Female , Guinea Pigs , Male , Mice , Rats , Species SpecificityABSTRACT
We studied the in vivo effect of rat immunization with tularemia live vaccine (TLV) on chromosomal aberrations (CA) induced in bone marrow cells by 4 anthracycline antibiotics. CA induced by adriamycin (ADR) and 4'-epiadriamycin (EADR) in rat bone marrow cells consisted mainly of chromatid breaks (approximately 90%), whereas lesions induced by aclacur (AC) and aclarubicin (ACR) consisted only of chromatid breaks. Preliminary cutaneous immunization of rats with TLV revealed significant suppression of CA induced by all 4 antibiotics. The present and previous results suggest that TLV may be a potent anticlastogenic factor.
