ABSTRACT
Molecular analysis of the LMP (latent membrane protein) oncogene was performed in 21 Epstein-Barr virus (EBV) positive cases of Hodgkin's disease (HD) with proven LMP gene expression. In each case, viral DNA of the LMP gene was assessed for polymorphism (deletions, insertions, mutations) by polymerase chain reaction (PCR) amplification with selected primers. Specificity of the amplified targets was proven by internal oligonucleotide hybridisation and nested primer PCR. Homogeneity of the 5' LMP gene region coding for the amino terminal, transmembrane, and short extracytoplasmic domains of the protein was identified in all cases. However, deletions or insertions of small DNA sequences within the coding region for the intracytoplasmic LMP domain were observed in about 20% of cases. In one of them, a 30-base-pair deletion was precisely localized by DNA sequencing. A particularly high frequency of DNA polymorphism (30% of cases) was found in the 3' untranslated LMP region. However, when analysing the LMP gene in seven benign conditions, no DNA polymorphism was found. These data suggest conservation of oncogenic LMP regions coding for the protein domains known to be associated with transforming capacities and immunogenic functions. They also show a considerable genomic heterogeneity of the coding region for the intracytoplasmic domain and the 3' untranslated mRNA region. This LMP DNA polymorphism identified within a localized (Swiss) population suffering from HD is unexpected. Its eventual clinical significance remains to be determined.
Subject(s)
Antigens, Viral/genetics , DNA-Binding Proteins/genetics , Hodgkin Disease/genetics , Base Sequence , Epstein-Barr Virus Nuclear Antigens , Humans , Molecular Sequence Data , Oligonucleotide Probes , Polymerase Chain ReactionABSTRACT
Desde enero 1986 a junio 1987 estudiamos 15 pacientes portadores de fascitis necrotizante. Siete hombres y 8 mujeres con edad promedio de 48,7 años (19 y 75 años). Estudiamos la localización, patología asociada, bacteriología, anatomía patológica, tratamiento y evolución. En 33% las lesiones se localizaron en pared abdominal, 44,6% en extremidades inferiores, 6,7% en cuello y tórax. El tiempo promedio entre el inicio de los síntomas y hospitalización fue de 7,4 días (3 y 15 días). Todos los pacientes se trataron con resección amplia y antibióticos parenterales. La asociación más frecuente fue de penicilina, gentamicina y metronidazol. En 12 de los 15 pacientes se obtuvo cultivo de herida operatoria: 11 fueron positivos y 1 negativo. En 3 no se practicó. Cinco pacientes fallecieron (33,3%), 2 antes de las 24 horas post ingreso y 3 antes del 7- día.
Subject(s)
Adult , Middle Aged , Humans , Male , Female , Anti-Bacterial Agents/therapeutic use , Fasciitis/surgery , Fasciitis/drug therapy , NecrosisABSTRACT
We report here a patient with Philadelphia chromosome (Ph)-positive chronic myelogenous leukemia (CML) in chronic phase in whom the alkaline phosphatase activity of neutrophils in the peripheral blood was low while at the same time the alkaline phosphatase content of neutrophils present in the urine was elevated. This observation provides independent clinical support for the recent experimental finding that an extrinsic factor (granulocyte colony-stimulating factor) controls alkaline phosphatase expression in human neutrophils.
Subject(s)
Alkaline Phosphatase/metabolism , Blood Cells/enzymology , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/enzymology , Neutrophils/enzymology , Urine/cytology , Aged , Female , HumansABSTRACT
On Surgical Clinic KBC and Medical faculty at Rijeka in the period from 30.01.1971 to 30.03.1988 403 kidney transplantations were performed. There were 257 (63.8%) transplant from living related donor, 2 (0.5%) from living nonrelated donor and 144 (35.7%) from cadaver. On 90 patients 104 reinterventions were performed. There were 29 reinterventions in 24 patients because of vascular complications. 14 patients lost their graft, and two patients died because of sepsis and liver failure. Among 140 patients with uretero-ureteral anastomoses 18 reinterventions were performed in 16 patients, patients lost the graft. In the group of 271 patients with ureterocystoneostomia there were 18 reinterventions in 17 patients. One patient died because of pulmonary emboli on the first postoperative day. Wound infections were surgically treated 39 times in 33 patients. 3 grafts were lost because of acute rupture, and one patient died in haemorrhagic shock. The complications after kidney transplantation should be recognized as soon as possible and if it is necessary to start surgical reintervention immediately.
