ABSTRACT
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Subject(s)
Female , Adult , Humans , Telangiectasia, Hereditary Hemorrhagic/complications , Brain Abscess/complications , Brain Abscess/drug therapy , Brain Abscess/surgerySubject(s)
Brain Abscess , Telangiectasia, Hereditary Hemorrhagic , Adult , Brain Abscess/diagnosis , Brain Abscess/etiology , Brain Abscess/microbiology , Brain Abscess/pathology , Female , Humans , Magnetic Resonance Imaging , Telangiectasia, Hereditary Hemorrhagic/complications , Telangiectasia, Hereditary Hemorrhagic/diagnosis , Telangiectasia, Hereditary Hemorrhagic/pathologyABSTRACT
Specific endothelin (ET) binding sites were characterized in membranes prepared from human cerebral cortices using binding assay and cross-linking analysis. The presence of immunoreactive (IR) ET-1 was studied by radioimmunoassay. Saturation binding experiments revealed that the KD and Bmax for 125I-ET-1 and 125I-ET-3 to membranes from gray matter were 25 +/- 6 pM and 115 +/- 15 fmol/mg of protein and 24 +/- 5 pM and 108 +/- 13 fmol/mg of protein, respectively. Similar results were obtained for white matter. In the presence of 10 nM sarafotoxin-6c, which is selective for ETB receptors, 125I-ET-1 and 125I-ET-3 binding was totally abolished. However, in the presence of 1 microM BQ123, which is selective for ETA receptors, both bindings were not affected. These results suggest that the human cerebral cortex contains only ETB receptors. Cross-linking of 125I-ET-1 and 125I-ET-3 to membranes with disuccinimidyl suberate resulted in the labeling of two bands of 48 and 31 kDa. Concentrations of IR-ET-1 in the gray and white matter were 7.0 +/- 3.2 and 2.5 +/- 1.7 fmol/g wet weight, respectively. The demonstration of high-affinity ETB receptors and the presence of IR-ET-1 suggest that the peptide may act as a neurotransmitter or neuromodulator in the human cerebral cortex.
Subject(s)
Cerebral Cortex/metabolism , Endothelins/metabolism , Receptors, Endothelin/metabolism , Binding, Competitive , Cell Membrane/metabolism , Cerebral Cortex/chemistry , Cross-Linking Reagents , Endothelins/analysis , Endothelins/pharmacology , Humans , Iodine Radioisotopes , Receptors, Endothelin/analysis , Succinimides , Viper Venoms/pharmacologyABSTRACT
A case of dural cavernous hemangioma, diagnosed prenatally as an intracranial mass by ultrasonography and surgically treated after elective delivery, is presented. The clinical and pathological features of this unusual type of congenital vascular malformation are reported, and the relevant literature is reviewed.
