ABSTRACT
Based on the performed studies, the authors developed test systems to analyze the expression of mRNA of the p53, C-myc, mad1, max, and E2F1 genes. These test systems could reveal a statistically significant difference between follicular adenoma and carcinoma of the thyroid in their expression of p53 mRNA. It should be noted that the use of our developed test systems is promising when searching for the diagnostic and prognostic markers of cancer, analyzing, and creating the genetic networks characterizing this or that cancer.
Subject(s)
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/biosynthesis , Cell Cycle Proteins/biosynthesis , E2F1 Transcription Factor/biosynthesis , Nuclear Proteins/biosynthesis , Proto-Oncogene Proteins c-myc/biosynthesis , Tumor Suppressor Protein p53/biosynthesis , Adenoma/metabolism , Biomarkers, Tumor/biosynthesis , Cell Line, Tumor , Humans , Reagent Kits, Diagnostic , Reverse Transcriptase Polymerase Chain Reaction , Thyroid Neoplasms/metabolismABSTRACT
Telomerase activity (TA) was examined in gastric adenocarcinomas and gastric lymphoma using a modified TRAP assay. TA was present in 16 of 18 (89%) gastric adenocarcinomas and in gastric lymphoma, whereas no TA was detected in normal tissue. Almost all samples had "high" and "very high" TA levels. Telomerase is undoubtedly associated with the process of malignant transformation and therefore can be an important marker for diagnostics of gastric cancer.
Subject(s)
Adenocarcinoma/enzymology , Biomarkers, Tumor/metabolism , Cell Transformation, Neoplastic/metabolism , Lymphoma/enzymology , Stomach Neoplasms/enzymology , Telomerase/metabolism , Adenocarcinoma/diagnosis , Adenocarcinoma/pathology , Cell Transformation, Neoplastic/pathology , Female , Humans , Lymphoma/diagnosis , Lymphoma/pathology , Male , Stomach Neoplasms/diagnosis , Stomach Neoplasms/pathologyABSTRACT
A correlation between the blood level of prostate-specific antigen (PSA) and the prostatic tissue activity of telomerase was analyzed in prostate cancer (PC), low- and high-grade prostatic intraepithelial neoplasia (LG PIN, HG PIN) and in benign prostatic hyperplasia (BPH). The study was based on the results of a comprehensive examination of 92 patients from the Clinic of Urology, I. M. Sechenov Moscow Medical Academy. 44% of the patients were diagnosed as having PC; 49% had LG PIN and BPH; 7% had HG PIN and BPH. Active telomerase in the prostate biopsy specimens was found in 98% of the patients with PC, in 33% of those with HG PIN, and in 20% of those with LG PIN. When active telomerase was detected in the prostate biopsy specimens of patients with urological cancer (PC, PIN, BPH, the blood content of total PSA) was statistically significantly higher than that in the absence of this enzyme.
Subject(s)
Prostate-Specific Antigen/blood , Prostatic Neoplasms/enzymology , Telomerase/metabolism , Biopsy , Humans , Male , Prostatic Neoplasms/pathology , Urologic Neoplasms/enzymology , Urologic Neoplasms/pathologyABSTRACT
The effect of antisense oligonucleotides complementary to the RNA component of human telomerase on telomerase activity in cell extracts of the melanoma cell line SK-Mel-28 has been studied. It has been shown that the antisense oligonucleotide complementary to the hTR component in the region of the template synthesis of telomeric repeats is the most efficient inhibitor of telomerase activity in comparison with other antisense oligonucleotides. Pronounced inhibition of telomerase activity was observed at the oligonucleotide (Tel P5) concentration in the reaction mixture of about 5 nM. Complete inhibition of the enzyme occurs at the oligonucleotide concentration in the sample of about 20 nM.
