Morphological diversity and fruit production of wild Salacia kraussii (Celastraceae) on the Northern Coast of KwaZulu-Natal, South Africa.

This study aimed to assess morphological diversity within Salacia kraussii, a fruit and medicinal wild plant species, based on morphological features and compared the fruit production among morphological types (morphotype) that naturally occur on the northern coast of KwaZulu-Natal. Following one species plant survey, a description of the qualitative morphological features revealed that S. kraussii individuals mainly differed in their leaf shapes, having elliptic, oblong, or obovate leaves. That led us to the identification of three morphotypes, namely Salacia kraussii 'elliptic', Salacia kraussii 'oblong' and Salacia kraussii 'obovate'. The analysis of variance (one-way ANOVA) of plant quantitative features indicated that plant height, stem diameter, branch number, leaf number and area, and fruit number were significantly different between plants from different sites (p-values < 0.05) and morphotypes (p-values < 0.01). Generally, S. kraussii grows in KwaZulu-Natal as a suffrutex with many stems and exhibits short plant height, small stem diameter, branches, and little foliage per stem. The average fruit number recorded per plant stem was likewise few. Plants growing in Sikhalasenkosi (site1) dominated in average plant height (35.58 cm), leaf number (45), number of branches (4), and number of fruits (5). Plants with elliptic leaves constantly dominated in average plant height (34.45 cm), foliage (36 leaves of 16.29 cm2 each), number of branches (4), and number of fruits (5). A few plants exhibited a strong vegetative vigor and produced more than 20 fruits. There was a highly positive correlation (CC = 0.8) between plant height and leaf number, branch number and leaf number, and branch number and fruit number. However, a negative correlation (CC = -0.1) was recorded between the leaf area and stem diameter. Overall, the study showed wide morphological diversity and fruit production within and between populations of S. kraussii, on the northern coast of KwaZulu-Natal.

Assessing Genetic Variation among Strychnos spinosa Lam. Morphotypes Using Simple Sequence Repeat Markers.

Strychnos spinosa Lam., commonly known as green monkey orange, is a highly valued indigenous fruit tree in South Africa with potential for domestication and commercialization. However, no study has reported on the molecular diversity of Strychnos spinosa morphotypes. Therefore, this study aimed to determine genetic variation among 32 Strychnos spinosa morphotypes using simple sequence repeat (SSR) markers. Fourteen amplified SSR markers produced 159 alleles, with a mean of 5.68 per locus. The polymorphic information content (PIC) values ranged from 0.22 (Ssp_1) to 0.84 (Ssp_6). Morphotypes were clustered in a biplot based on their genetic distances. The dendrogram chiefly discriminated morphotypes according to variation of pericarp texture. The population structure had the highest delta value K = 3, thus the 32 morphotypes were divided into three subpopulations based on the Bayesian approach. The affinities produced by the population structure agreed with the genetic distance of closely related morphotypes. This study is the first to report on SSR marker development and their successful use for genetic diversity and population structure studies of Strychnos spinosa. It provides insights into the molecular characterisation of Strychnos spinosa. This can lead to breeding programs and crop improvement programs, particularly in varietal developmental programs, which can contribute to alleviating food security challenges.

'To prevent this disease, we have to stay at home, but if we stay at home, we die of hunger' - Livelihoods, vulnerability and coping with Covid-19 in rural Mozambique.

Non-pharmaceutical interventions (NPIs) such as social distancing and travel restrictions have been introduced to prevent the spread of the novel coronavirus (hereinafter Covid). In many countries of the Global South, NPIs are affecting rural livelihoods, but in-depth empirical data on these impacts are limited. We traced the differentiated impacts of Covid NPIs throughout the start of the pandemic May to July 2020. We conducted qualitative weekly phone interviews (n = 441) with 92 panelists from nine contrasting rural communities across Mozambique (3-7 study weeks), exploring how panelists' livelihoods changed and how the NPIs intersected with existing vulnerabilities, and created new exposures. The NPIs significantly re-shaped many livelihoods and placed greatest burdens on those with precarious incomes, women, children and the elderly, exacerbating existing vulnerabilities. Transport and trading restrictions and rising prices for consumables including food meant some respondents were concerned about dying not of Covid, but of hunger because of the disruptions caused by NPIs. No direct health impacts of the pandemic were reported in these communities during our interview period. Most market-orientated income diversification strategies largely failed to provide resilience to the NPI shocks. The exception was one specific case linked to a socially-minded value chain for baobab, where a strong duty of care helped avoid the collapse of incomes seen elsewhere. In contrast, agricultural and charcoal value chains either collapsed or saw producer prices and volumes reduced. The hyper-covariate, unprecedented nature of the shock caused significant restrictions on livelihoods through trading and transport limits and thus a region-wide decline in cash generation opportunities, which was seen as being unlike any prior shock. The scale of human-made interventions and their repercussions thus raises questions about the roles of institutional actors, diversification and socially-minded trading partners in addressing coping and vulnerability both conceptually and in policy-making.