ABSTRACT
We examined the effects of elevated temperatures and biocides on survivability of food isolates of Cronobacter spp. (C. sakazakii) and concomitant enterobacteriaceae obtained in microbiological control of infant nutrition products. Increased resistance of certain strains of Cronobacter, Enterobacter cloacae, and Pantoea spp. to thermal processing was revealed. Salmonella, Pantoea, and Cronobacter bacteria were least sensitive to antimicrobial action of chlorine-containing agents. The above properties varied in the strains of the same species. Specifically, only two of three examined isolates of Cronobacter spp. demonstrated lower sensitivity to heat in comparison with the enterobacterial test-cultures of other species.
Subject(s)
Chlorine , Cronobacter , Disinfectants , Food Microbiology , Disinfectants/pharmacology , Cronobacter/drug effects , Cronobacter/isolation & purification , Chlorine/pharmacology , Enterobacteriaceae/drug effects , Enterobacteriaceae/isolation & purification , Hot Temperature , Humans , Cronobacter sakazakii/drug effects , Cronobacter sakazakii/isolation & purification , Microbial Sensitivity Tests , Salmonella/drug effects , Salmonella/isolation & purification , Enterobacter cloacae/drug effects , Enterobacter cloacae/isolation & purificationABSTRACT
Extracellular and intracellular hydrocarbons produced by Clostridium pasteurianum VKM 1774 during cultivation on glucose-containing media in an argon atmosphere or in the presence of carbon dioxide and molecular hydrogen were analyzed by gas-liquid chromatography. Intracellular hydrocarbons were 50-55% (C25-C35) n-alkanes. Carbon dioxide and molecular hydrogen stimulated synthesis of extracellular hydrocarbons, which comprised 90-95% (C11-C24) n-alkanes.