Histochemical analysis of nasal polyps.

In the last 3 years, 86 nasal polyps from 76 patients were examined. Special attention was paid to the group of recurrent nasal polyps, which redeveloped from 2 to 17 times. Histological analysis was performed concerning the eosinophils, mast cells, acid and neutral mucopolysaccharides, acetylcholinesterase and fluorescence on monoamines. Macroscopically, nasal mucosa in 53 patients was considered for control purposes. The comparison was made and the results of the examination reviewed.

Comparative analysis of human nasal mucosa by standard neurohistochemical techniques.

The modified glyoxylic acid histofluorescent method (Torre and Surgeon) and Karnovsky-Roots method for acetylcholinesterase activity was applied to fresh animal and human material. Human material was obtained during ENT surgery and consisted of nasal, laryngeal and hypopharyngeal mucosa. Not only on animal, but also on human material adrenergic axons and varicosities were demonstrated around vessels in nasal mucosa, diffusely in nasal, laryngeal and hypopharyngeal mucosa and just below and intraepithelially in laryngeal mucosa. Abundant cholinergic innervation was found around vessels and glands in nasal, laryngeal and hypopharyngeal mucosa, as well as diffusely scattered in the tissue of their mucosa.

[Incus homograft--histological study of implanted ossicles (author's transl)]. / Die Homoiotransplantation des Ambosses Histologische Untersuchungen der implantierten Knöchelchen

The methodes of obtaining, preparing and storing of ossicles for homoiotransplantation is described. Using this method, 21 patients have been operated. Four reoperations have been performed, and in two cases implants were found and subsequently subjected to histological analysis. The method of preparing the histological slides is described. The study of examined homografts revealed the normal bone structure and signs of revitalisation, which is clearly shown in the micrographs. On the basis of functional results and histological examination, the authors conclude that the use of devitalised homografts is recommanded in total and/or partial defects of ossicles.

Histology of the mucous membranes of the lateral nasal wall.

The preliminary results of investigating the lateral nasal wall are presented. In this study only three strips of mucosa are analysed from histological point of view using serial sections. One strip corresponded to the plane of lateral osteotomy, the second crossed the middle of turbinates and the third corresponded to the vestibular area. The accurate number and distribution of vascular and glandular elements were determined by help of Visopan and micro-photographs.