ABSTRACT
Purpose. To evaluate aqueous humor MMP-9 levels in alkali-burned rabbit cornea following KPr implantation and their roles in RPMs formation. Methods. Left eyes of 36 rabbits received a deep corneal alkali wound. 12 corneas were implanted with KPro and the other 24 control corneas were either penetrating keratoplasty or left without keratoplasty. Aqueous humor MMP-9 and TIMP-1 levels were determined and RPMs were obtained for histopathological and ultrastructural examination. Results. Alkali exposure induced significant increase in aqueous humor MMP-9 level and the data were further enhanced by KPro implantation. By contrast, TMIP-1 levels in aqueous humor showed a decreased trend following corneal alkali burn and KPro surgery. RPMs were developed in 5 out of 10 cases of KPro successfully implanted eyes. Histopathology showed the presence of a large number of fibroblasts and collagen fibers arranged irregularly with inflammatory cells infiltration, and an ingrowth of new blood vessels in this retrokeratoprosthesis fibrous tissue. Immunohistochemical analysis showed positive stain of RPMs for both MMP-9 and TIMP-1. Aqueous humor MMP-9 levels were significantly higher in RPM group postoperatively, while TIMP-1 levels were comparatively lower than that of No-RPM group. Conclusions. Our study evidenced the potential pathophysiological role of MMP-9 expression in RPM formation following KPro implantation.
ABSTRACT
PURPOSE: To study surgical debridement in the management of fungal keratitis in 44 patients. DESIGN: A prospective randomized study. METHODS: Patients with presumed fungal keratitis were randomized into 2 groups. The first group had diagnostic corneal scraping with a Kimura spatula while the second group underwent surgical debridement utilizing a motorized drill with attached diamond burr. Material obtained from both methods was plated on Sabaurauds medium, blood agar and stained for bacteria and fungi. Topical and systemic antifungal therapy was instituted, and patients were examined until resolution. RESULTS: Pathogens were identified to a greater degree in the aggressive debridement group than in the Kimura spatula group. Patients who had aggressive debridement had a significant reduction in time to re-epithelialization and keratitis resolution (9.4 days) compared with the scraping group (17.1 days, P < 0.001). Mean uncorrected visual acuity (UCVA) in the surgical group was 1.02 logarithm of the minimal angle of resolution (logMAR) preoperatively and 0.43 logMAR postoperatively (P < 0.001). Mean UCVA in the Kimura spatula group was 1.20 logMAR before and 0.58 logMAR after scraping (P = 0.03). A single incidence of perforation occurred in the scraping group with the diagnosis of Fusarium made from the corneal button. CONCLUSIONS: Aggressive debridement and debulking of fungal keratitis provides a faster clinical resolution with no reduction in visual potential. The acquisition of pathogens is more accurate with surgical debridement as compared with conventional scraping. Aggressive surgical debridement of keratitis not only aids in diagnosis but may also be therapeutic.
ABSTRACT
PURPOSE: To illustrate the implications of the unauthorized sale and unmonitored wearing of decorative cosmetic contact lenses resulting in ocular complications and to determine the prevalence of the use of cosmetic contact lenses obtained from unlicensed providers by adolescents. METHODS: Observational case report, structured interview, and survey in a retrospective, observational, clinical practice setting. Reported sources of contact lenses were categorized as provider and nonprovider, and associations within the data were reviewed by using a Pearson correlation and chi-square test. RESULTS: Twelve patients (eight female and four male) were seen urgently for acute eye pain and redness after wearing plano decorative contact lenses. None of the patients had previously worn a contact lens. None of the lenses were dispensed by eye care professionals. Four patients developed blinding infections requiring hospital admission. Causative organisms included staphylococci, Pseudomonas, and acanthamoeba. One patient required a penetrating keratoplasty. One hundred fifty-nine patients were surveyed. Thirty-seven (23%) used decorative contact lenses. Lenses were obtained from an unlicensed provider 51% of the time. Education about lens care and handling was significantly associated with acquiring lenses from licensed providers (R = 0.74, P < 0.0001). CONCLUSIONS: Colored noncorrective contact lenses are being dispensed without a prescription or fitting from unlicensed vendors. Patients who acquire lenses from unauthorized providers are significantly less likely to be instructed on appropriate lens use and care. Consequently, uninformed lens wearers are experiencing acute vision-threatening infections and inflammation.
