Tannic acid-modified silver nanoparticles enhance the anti-Acanthamoeba activity of three multipurpose contact lens solutions without increasing their cytotoxicity.

BACKGROUND: Free-living amoebae of the genus Acanthamoeba are cosmopolitan, widely distributed protozoans that cause a severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). The majority of the increasing number of AK cases are associated with contact lens use. Appropriate eye hygiene and effective contact lens disinfection are crucial in the prevention of AK because of the lack of effective therapies against it. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent AK infections. Synthesized nanoparticles (NPs) have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that some plant metabolites, including tannins, have anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of tannic acid-modified silver NPs (AgTANPs) conjugated with selected multipurpose contact lens solutions. METHODS: The anti-amoebic activities of pure contact lens care solutions, and NPs conjugated with contact lens care solutions, were examined in vitro by a colorimetric assay based on the oxido-reduction of alamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. The results were statistically analysed by ANOVA and Student-Newman-Keuls test using P < 0.05 as the level of statistical significance. RESULTS: We show that the NPs enhance the anti-Acanthamoeba activities of the tested contact lens solutions without increasing their cytotoxicity profiles. The activities are enhanced within the minimal disinfection time recommended by the manufacturers. CONCLUSIONS: The conjugation of the selected contact lens solutions with AgTANPs might be a novel and promising approach for the prevention of AK infections among contact lens users.

Positive psychotherapy for psychosis - a new approach in the rehabilitation of patients suffering from schizophrenia. / Pozytywna Psychoterapia Psychoz ­ nowe podejscie w rehabilitacji pacjentów cierpiacych na schizofrenie.

This paper describes the positive psychotherapy for psychosis (PPP) - a new approach for psychiatric rehabilitation for patients with chronic schizophrenia. Unlike some traditional methods of psychotherapy, PPP focuses on positives rather than on problems. PPPwas shown in the context of other therapeutic approaches used in psychiatric rehabilitation and a mechanism of changes in the functioning and thinking of patients using this approach was described. PPP strengthens the patients'resources, including positive emotions, positive features of character, sense of life, positive relationships, and internal motivation. PPPdoes not suggest that other approaches are inappropriate and it is not intended to replace well-established treatments. A program of 13 PPP sessions adapted for patients with schizophrenia was also described. PPP sessions were divided into 3 thematic groups and according to the degree of difficulty: "the easiest" (savoring, positive things - sessions 1-4), "medium" (strong character traits, recognition of strengths in oneself, perceiving strengths in other people, positive communication - sessions 5-7) and "the most difficult" (bad vs. good memories, gratitude, forgiveness, hope, optimism and posttraumatic growth - sessions 8-13). The PPP has been introduced in the Day Care Department of Psychiatric Rehabilitation of the Institute of Psychiatry and Neurology in Warsaw. After 13 weeks, the initial observations were collected. They indicated that PPP can be successfully used in psychiatric rehabilitation.

Strengths in patients with schizophrenia and healthy people - similarities and differences. / Mocne strony osób chorych na schizofrenie i osób zdrowych ­ podobienstwa i róznice.

OBJECTIVES: The aim of the study was to assess the character strengths of people with schizophrenia and their importance in building life satisfaction, and also to identify differences and similarities in the character strengths between people with schizophrenia and healthy people. METHODS: 40 people with diagnosed schizophrenia and 40 healthy people participated in the study. Each participant was examined with the following tools: Socio-Demographic Data Questionnaire, Satisfaction with Life Scale (SWLS), International Personality Item Pool - Values in Action (IPIP-VIA). RESULTS: People with schizophrenia achieved significantly lower scores than those in the control group in terms of such strengths of character like: hope, humor, self-regulation, citizenship, leadership, capacity for love, kindness, social/emotional intelligence, bravery, industry, integrity, zest, curiosity, love of learning, perspective/wisdom. People with schizophrenia are also different from healthy people in terms of experiencing life satisfaction. At the same time, they do not differ in such strengths of character as: originality, judgment, fairness, forgiveness/mercy, modesty/humility, prudence, appreciation for beauty, gratitude, spirituality. The character strengths of people in the clinical group (more often than in the control group) are associated with the sense of life satisfaction. In older people suffering from schizophrenia, many strengths of their character are reduced. CONCLUSIONS: People with schizophrenia are different from those in the control group in terms of 15 strengths. They are also less satisfied with their lives. Experiencing life satisfaction of people with schizophrenia is associated with a complex configuration of strengths.

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains.

INTRODUCTION: Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease - Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites. MATERIAL AND METHODS: Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25-2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line. RESULTS: Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed. CONCLUSIONS: Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent Acanthamoeba keratitis among contact lens users.