Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 9 de 9
Filter
Add more filters










Database
Language
Publication year range
1.
Front Immunol ; 4: 268, 2013 Sep 12.
Article in English | MEDLINE | ID: mdl-24062738

ABSTRACT

Adoptive cell transfer using engineered T cells is emerging as a promising treatment for metastatic melanoma. Such an approach allows one to introduce T cell receptor (TCR) modifications that, while maintaining the specificity for the targeted antigen, can enhance the binding and kinetic parameters for the interaction with peptides (p) bound to major histocompatibility complexes (MHC). Using the well-characterized 2C TCR/SIYR/H-2K(b) structure as a model system, we demonstrated that a binding free energy decomposition based on the MM-GBSA approach provides a detailed and reliable description of the TCR/pMHC interactions at the structural and thermodynamic levels. Starting from this result, we developed a new structure-based approach, to rationally design new TCR sequences, and applied it to the BC1 TCR targeting the HLA-A2 restricted NY-ESO-1157-165 cancer-testis epitope. Fifty-four percent of the designed sequence replacements exhibited improved pMHC binding as compared to the native TCR, with up to 150-fold increase in affinity, while preserving specificity. Genetically engineered CD8(+) T cells expressing these modified TCRs showed an improved functional activity compared to those expressing BC1 TCR. We measured maximum levels of activities for TCRs within the upper limit of natural affinity, K D = ∼1 - 5 µM. Beyond the affinity threshold at K D < 1 µM we observed an attenuation in cellular function, in line with the "half-life" model of T cell activation. Our computer-aided protein-engineering approach requires the 3D-structure of the TCR-pMHC complex of interest, which can be obtained from X-ray crystallography. We have also developed a homology modeling-based approach, TCRep 3D, to obtain accurate structural models of any TCR-pMHC complexes when experimental data is not available. Since the accuracy of the models depends on the prediction of the TCR orientation over pMHC, we have complemented the approach with a simplified rigid method to predict this orientation and successfully assessed it using all non-redundant TCR-pMHC crystal structures available. These methods potentially extend the use of our TCR engineering method to entire TCR repertoires for which no X-ray structure is available. We have also performed a steered molecular dynamics study of the unbinding of the TCR-pMHC complex to get a better understanding of how TCRs interact with pMHCs. This entire rational TCR design pipeline is now being used to produce rationally optimized TCRs for adoptive cell therapies of stage IV melanoma.

2.
J Mol Recognit ; 23(2): 142-52, 2010.
Article in English | MEDLINE | ID: mdl-20151417

ABSTRACT

Recognition by the T-cell receptor (TCR) of immunogenic peptides (p) presented by class I major histocompatibility complexes (MHC) is the key event in the immune response against virus infected cells or tumor cells. The major determinant of T cell activation is the affinity of the TCR for the peptide-MHC complex, though kinetic parameters are also important. A study of the 2C TCR/SIYR/H-2Kb system using a binding free energy decomposition (BFED) based on the MM-GBSA approach had been performed to assess the performance of the approach on this system. The results showed that the TCR-p-MHC BFED including entropic terms provides a detailed and reliable description of the energetics of the interaction (Zoete and Michielin, 2007). Based on these results, we have developed a new approach to design sequence modifications for a TCR recognizing the human leukocyte antigen (HLA)-A2 restricted tumor epitope NY-ESO-1. NY-ESO-1 is a cancer testis antigen expressed not only in melanoma, but also on several other types of cancers. It has been observed at high frequencies in melanoma patients with unusually positive clinical outcome and, therefore, represents an interesting target for adoptive transfer with modified TCR. Sequence modifications of TCR potentially increasing the affinity for this epitope have been proposed and tested in vitro. T cells expressing some of the proposed TCR mutants showed better T cell functionality, with improved killing of peptide-loaded T2 cells and better proliferative capacity compared to the wild type TCR expressing cells. These results open the door of rational TCR design for adoptive transfer cancer therapy.


Subject(s)
Major Histocompatibility Complex , Peptides/chemistry , Receptors, Antigen, T-Cell/chemistry , HLA-A2 Antigen/chemistry , HLA-A2 Antigen/immunology , Humans , Models, Molecular , Peptides/immunology , Protein Binding/immunology , Protein Structure, Tertiary , Receptors, Antigen, T-Cell/immunology
3.
J Comput Aided Mol Des ; 17(12): 861-80, 2003 Dec.
Article in English | MEDLINE | ID: mdl-15124934

ABSTRACT

A method is proposed for the estimation of absolute binding free energy of interaction between proteins and ligands. Conformational sampling of the protein-ligand complex is performed by molecular dynamics (MD) in vacuo and the solvent effect is calculated a posteriori by solving the Poisson or the Poisson-Boltzmann equation for selected frames of the trajectory. The binding free energy is written as a linear combination of the buried surface upon complexation, SASbur, the electrostatic interaction energy between the ligand and the protein, Eelec, and the difference of the solvation free energies of the complex and the isolated ligand and protein, deltaGsolv. The method uses the buried surface upon complexation to account for the non-polar contribution to the binding free energy because it is less sensitive to the details of the structure than the van der Waals interaction energy. The parameters of the method are developed for a training set of 16 HIV-1 protease-inhibitor complexes of known 3D structure. A correlation coefficient of 0.91 was obtained with an unsigned mean error of 0.8 kcal/mol. When applied to a set of 25 HIV-1 protease-inhibitor complexes of unknown 3D structures, the method provides a satisfactory correlation between the calculated binding free energy and the experimental pIC5o without reparametrization.


Subject(s)
HIV-1/enzymology , Protease Inhibitors/metabolism , Protein Binding/physiology , Proteins/metabolism , HIV-1/metabolism , Humans , Kinetics , Ligands , Static Electricity
4.
J Mol Biol ; 315(1): 21-52, 2002 Jan 04.
Article in English | MEDLINE | ID: mdl-11771964

ABSTRACT

The flexibility of different regions of HIV-1 protease was examined by using a database consisting of 73 X-ray structures that differ in terms of sequence, ligands or both. The root-mean-square differences of the backbone for the set of structures were shown to have the same variation with residue number as those obtained from molecular dynamics simulations, normal mode analyses and X-ray B-factors. This supports the idea that observed structural changes provide a measure of the inherent flexibility of the protein, although specific interactions between the protease and the ligand play a secondary role. The results suggest that the potential energy surface of the HIV-1 protease is characterized by many local minima with small energetic differences, some of which are sampled by the different X-ray structures of the HIV-1 protease complexes. Interdomain correlated motions were calculated from the structural fluctuations and the results were also in agreement with molecular dynamics simulations and normal mode analyses. Implications of the results for the drug-resistance engendered by mutations are discussed briefly.


Subject(s)
HIV Protease Inhibitors/chemistry , HIV Protease Inhibitors/metabolism , HIV Protease/chemistry , HIV Protease/metabolism , Amino Acid Substitution , Binding Sites , Computer Simulation , Consensus Sequence , Crystallography, X-Ray , Databases, Protein , Ligands , Models, Molecular , Motion , Mutation , Pliability , Protein Binding , Protein Conformation
5.
FEBS Lett ; 486(1): 19-22, 2000 Dec 01.
Article in English | MEDLINE | ID: mdl-11108835

ABSTRACT

4-Mercaptoimidazoles derived from the naturally occurring antioxidants, ovothiols, were tested for their glutathione peroxidase-like (GSH Px-like) activity and protection against peroxynitrite-induced damage. All the thiol compounds displayed similar significant GSH Px-like activities, which are however weaker than that of the reference compound, ebselen. The inhibitions of the peroxynitrite-dependent oxidation of Evans blue dye and dihydrorhodamine 123 showed that the thiol compounds substituted on position 5 of the imidazole ring were nearly as effective as ebselen while the C-2 substituted ones were less effective. Both assays corroborate the large superiority of mercaptoimidazoles over glutathione as inhibitors of peroxynitrite-dependent oxidation.


Subject(s)
Antioxidants/metabolism , Glutathione Peroxidase/metabolism , Imidazoles/metabolism , Methylhistidines/metabolism , Nitrates/antagonists & inhibitors , Sulfhydryl Compounds/metabolism , Antioxidants/chemistry , Antioxidants/pharmacology , Azoles/metabolism , Azoles/pharmacology , Catalysis/drug effects , Chromans/metabolism , Chromans/pharmacology , Evans Blue/metabolism , Free Radical Scavengers/metabolism , Free Radical Scavengers/pharmacology , Glutathione/metabolism , Glutathione/pharmacology , Hydrogen Peroxide/metabolism , Imidazoles/chemistry , Imidazoles/pharmacology , Inhibitory Concentration 50 , Isoindoles , Kinetics , NADP/metabolism , Nitrates/metabolism , Nitrates/pharmacology , Organoselenium Compounds/metabolism , Organoselenium Compounds/pharmacology , Oxidants/antagonists & inhibitors , Oxidants/metabolism , Oxidants/pharmacology , Oxidation-Reduction/drug effects , Rhodamines/metabolism , Sulfhydryl Compounds/chemistry , Sulfhydryl Compounds/pharmacology
6.
Free Radic Biol Med ; 28(11): 1638-41, 2000 Jun 01.
Article in English | MEDLINE | ID: mdl-10938460

ABSTRACT

Using semi-empirical AM1 calculation and 6.31G* basis sets, we have calculated the energy of the highest-occupied molecular orbital (E(HOMO)) for anti-inflammatory 4,5-diarylpyrroles which have been shown to have inhibitory activity on cyclooxygenase COX-2, an inducible enzyme expressed during inflammation. We have found a correlation between the E(HOMO) of a molecule and its COX-2 inhibition. However, no correlation was observed between E(HOMO) and the inhibition efficiency of cyclooxygenase-1 (COX-1), the constitutively expressed enzyme, protective to the organism. This result suggests that the inhibitions of the two isoforms follow different molecular mechanisms.


Subject(s)
Cyclooxygenase Inhibitors/chemistry , Cyclooxygenase Inhibitors/pharmacology , Isoenzymes/antagonists & inhibitors , Pyrroles/pharmacology , Anti-Inflammatory Agents, Non-Steroidal/chemistry , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Cyclooxygenase 1 , Cyclooxygenase 2 , Cyclooxygenase 2 Inhibitors , In Vitro Techniques , Isoenzymes/pharmacology , Oxidation-Reduction , Prostaglandin-Endoperoxide Synthases/pharmacology , Pyrroles/chemistry , Structure-Activity Relationship , Thermodynamics
7.
Free Radic Res ; 32(6): 515-24, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10798717

ABSTRACT

4-Mercaptoimidazoles derived from the naturally occurring family of antioxidants, the ovothiols, were assayed for their antioxidant properties. These compounds are powerful HOCl scavengers, more potent than the aliphatic thiol N-acetylcysteine. They react slowly with hydrogen peroxide with second order rate constants of 0.13-0.89 M(-1)s(-1). Scavenging of hydroxyl radical occurs at a diffusion-controlled rate (k=2.0-5.0 x 10(10)M(-1)s(-1)) for the most active compounds, which are also able to inhibit copper-induced LDL peroxidation. The combination of radical scavenging and copper chelating properties may explain the inhibitory effects on LDL peroxidation. Two molecules of mercaptoimidazole can chelate a copper ion and form a square planar complex detected by EPR. Compounds bearing an electron-withdrawing group on position 2 of the imidazole ring are the most potent antioxidant molecules in this series.


Subject(s)
Antioxidants/pharmacology , Imidazoles/chemistry , Imidazoles/pharmacology , Methylhistidines/chemistry , Sulfhydryl Compounds/chemistry , Chelating Agents , Copper/chemistry , Copper/pharmacology , Free Radical Scavengers , Hydrogen Peroxide/chemistry , Hydroxyl Radical/chemistry , Hypochlorous Acid/chemistry , Lipid Peroxidation , Lipoproteins, LDL/chemistry , Oxidation-Reduction
8.
Free Radic Res ; 32(6): 525-33, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10798718

ABSTRACT

The radical-scavenging mechanism of fourteen 4-mercaptoimidazoles, derived from the natural family of ovothiols, was studied via a QSAR approach, cyclic voltammetry, ESR and NMR spectroscopy. A significant correlation was found between the DPPH scavenging abilities of test compounds and thermodynamic parameters like overall ease of disulphide formation. The production of a disulphide compound via thiyl radical formation is proposed. Upon DPPH scavenging, hydrogen abstraction from thiols yields transient short-lived thiyl radicals, which were characterised by ESR and rapidly dimerise to form a disulphide compound. Cyclic voltammetry showed that the best DPPH scavengers exhibit low oxidation potentials for their oxidation to disulphides.


Subject(s)
Antioxidants/chemistry , Free Radical Scavengers , Imidazoles/chemistry , Methylhistidines/chemistry , Picrates , Sulfhydryl Compounds/chemistry , Bepridil/analogs & derivatives , Bepridil/chemistry , Biphenyl Compounds , Electrochemistry , Electron Spin Resonance Spectroscopy , Free Radicals , Hydroxyl Radical/chemistry , Imidazoles/pharmacology , Molecular Structure , Structure-Activity Relationship , Thermodynamics
9.
Free Radic Biol Med ; 26(9-10): 1261-6, 1999 May.
Article in English | MEDLINE | ID: mdl-10381198

ABSTRACT

Using a simple quantum mechanical method, we calculated the energy of the highest-occupied molecular orbital (E(HOMO)) of three groups of anti-inflammatory compounds, and we have found correlations between E(HOMO) of these molecules and experimental data previously reported on (1) inhibition of sheep-vesicular-gland prostaglandin cyclooxygenase by phenolic compounds, (2) inhibition of prostaglandin cyclooxygenase in mouse macrophages by salicylates, benzoates and phenols, and (3) peroxyl-radical scavenging and radioprotection of a bacterial virus by NSAID drugs, including metiazinic acid, sulindac, D-penicillamine, piroxicam, indomethacin, benoxaprofen, and aspirin. Our correlations using a systematic evaluation of the HOMO energies can be of predictive value in the search for new anti-inflammatory drugs as well as for new radioprotectors.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/chemistry , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Animals , Bacteriophages/drug effects , Bacteriophages/radiation effects , Cyclooxygenase Inhibitors/chemistry , Cyclooxygenase Inhibitors/pharmacology , Free Radical Scavengers/chemistry , Free Radical Scavengers/pharmacology , In Vitro Techniques , Macrophages/drug effects , Macrophages/enzymology , Mice , Models, Chemical , Quantum Theory , Sheep , Structure-Activity Relationship , Thermodynamics
SELECTION OF CITATIONS
SEARCH DETAIL
...