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1.
Biull Eksp Biol Med ; 92(12): 743-7, 1981 Dec.
Article in Russian | MEDLINE | ID: mdl-7034808

ABSTRACT

Reactive modifications of synapses of isolated intratrunkal vegetative neurones induced by high frequency orthodromal electric stimulation have been examined by vital phase-contrast microscopy, electron microscopy, vital staining with methylene blue in conjunction with concurrent electrophysiological control Flocculent aggregation of the synaptoplasma and vesicles has been discovered, which is associated with higher adhesiveness of modified proteins. There have been also discovered matrix translucence and rounding of the living synaptic buds, which is accounted for by segregation of the hydration fraction of the neuroplasma. The decrease in the number of light synaptic vesicles is accompanied by the appearance of osmiophilic, myelin-like bodies, dense-core vesicles and glycogen-like granules. An attempt has been made to validate the process in terms of the colloid-chemical conception.


Subject(s)
Autonomic Nervous System/physiology , Action Potentials , Animals , Anura , Autonomic Nervous System/ultrastructure , Cats , Electric Stimulation , Electrophysiology , Methylene Blue/metabolism , Microscopy, Electron , Microscopy, Phase-Contrast , Synapses/physiology , Synapses/ultrastructure
2.
Vopr Onkol ; 25(7): 35-9, 1979.
Article in Russian | MEDLINE | ID: mdl-483678

ABSTRACT

Cytomorphometric examination of the normal urothelium, 3 primary, 3 recurrent papillomas of the urinary bladder and 3 cancers of stage I indicated that average relative volumes of the nucleus and cytoplasm in their cells are nearly similar. Since these bolumes increase simultaneously in the tumors concerned, there is no change in the "nucleus-cytoplasm correlation" or "index" described above. Cytomorphometric indices of the nucleus and cytoplasma in papillomas are more close to those of high-differentiated cancer than of the normal urothelium. In histologically indistinguishable primary and recurrent transitional cell papillomas the indices would differ: in primary papillomas compared with the norma the amount of mitochondria is reliably increased statistically, but the volume of each of them is diminished, whereas in recurrent ones--vice versa.


Subject(s)
Carcinoma, Transitional Cell/ultrastructure , Urinary Bladder Neoplasms/ultrastructure , Cell Nucleus/ultrastructure , Cytoplasm/ultrastructure , Epithelium/ultrastructure , Humans , Neoplasm Recurrence, Local/ultrastructure , Papilloma/ultrastructure , Urinary Bladder/ultrastructure
3.
Arkh Anat Gistol Embriol ; 71(8): 15-21, 1976 Aug.
Article in Russian | MEDLINE | ID: mdl-985091

ABSTRACT

The fine structure of cells of the conglomerates formed after dissociation of tissues of fresh-water sponges has been studied. In the development of the conglomerates of Ephydatia fluviatilis an important role is played by amoebocytes and choanocytes. In amoebocytes the shape of the nucleus, the mitochondria structure, the Golgi complex and endoplasmic reticulum have been found to change. In choanocytes, in addition to changes of organoids, the structure of the collar and flagella are subjected to desorganization. They have great plasticity and are able to re-differentiate in pinacocytes according to their position in the conglomerates. The transformation of amoebocytes and choanocytes into pinacocytes is accompanied by a successive modification of the nucleus and the cytoplasmic structures. In redifferentiation of amoebocytes the amount of Golgi apparatus zones decreased, the content of vacuoles of different size and the amount of elements of the agranular endoplasmic reticulum increased, and the defined nucleus disappeared.


Subject(s)
Porifera/cytology , Animals , Microscopy, Electron , Porifera/ultrastructure
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