Application of Dual-Frequency Self-Injection Locked DFB Laser for Brillouin Optical Time Domain Analysis.

Self-injection locking to an external fiber cavity is an efficient technique enabling drastic linewidth narrowing of semiconductor lasers. Recently, we constructed a simple dual-frequency laser source that employs self-injection locking of a DFB laser in the external ring fiber cavity and Brillouin lasing in the same cavity. The laser performance characteristics are on the level of the laser modules commonly used with BOTDA. The use of a laser source operating two frequencies strongly locked through the Brillouin resonance simplifies the BOTDA system, avoiding the use of a broadband electrooptical modulator (EOM) and high-frequency electronics. Here, in a direct comparison with the commercial BOTDA, we explore the capacity of our low-cost solution for BOTDA sensing, demonstrating distributed measurements of the Brillouin frequency shift in a 10 km sensing fiber with a 1.5 m spatial resolution.

Mitochondrial dependent oxidative stress in cell culture induced by laser radiation at 1265 nm.

Photodynamic therapy is the main technique applied for surface carcinoma treatment. This technique employs singlet oxygen generated via a laser excited photosensitizer as a main damaging agent. However, prolonged sensitivity to intensive light, relatively low tissue penetration by activating light the cost of photosensitizer (PS) administration can limit photodynamic therapy applications. Early was reported singlet oxygen generation without photosensitizer induced by a laser irradiation at the wavelength of 1250-1270 nm. Here, we study the dynamics of oxidative stress, DNA damage, changes of mitochondrial potential, and mitochondrial mass induced by a laser at 1265 nm have been studied in HCT-116 and CHO-K cells. Laser irradiation of HCT-116 and CHO-K cells has induced a dose-dependent cell death via increasing intracellular reactive oxygen species (ROS) concentration, increase of DNA damage, decrease of mitochondrial potential, and reduced glutathione. It has been shown that, along with singlet oxygen generation, the increase of the intracellular ROS concentration induced by mitochondrial damage contributes to the damaging effect of the laser irradiation at 1265 nm.