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Prikl Biokhim Mikrobiol ; 36(4): 428-32, 2000.
Article in Russian | MEDLINE | ID: mdl-10994191

ABSTRACT

Polyclonal rabbit antibodies against a conjugate synthesized through condensing BSA and disubstituted roridin A hemisuccinate allowed roridin A to be determined in solutions at a sensitivity of 0.2 ng/ml. The cross-reactivity of structural analogues--roridin A, verrucarin, and verrucarol--amounted to 100, 2.5, and 0.03%, respectively. The data showed that these antibodies determine roridin A in an indirect heterogeneous enzyme immunoassay in cereal straw samples at a sensitivity of 20 micrograms/kg.


Subject(s)
Antibodies/immunology , Antibody Formation , Mycotoxins/immunology , Animals , Cross Reactions , Mycotoxins/chemistry , Rabbits , Trichothecenes/chemistry , Trichothecenes/immunology
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