ABSTRACT
We studied an original radiolabeled complex of antimicrobial peptides UBI29-41 and UBI18-35, ubiquicidin derivatives, for distinguishing between bacterial and aseptic inflammation. For radiolabeling of the peptides with technetium-99m, a bifunctional chelating agent succinimide-1-yl 6-(bis(pyridin-2-ylmethyl)amino)hexanoate was used. The obtained complexes 99mТÑ-DPAH-UBI29-41 and 99mТÑ-DPAH-UBI18-35 had radiolabeling yield >80% and radiochemical purity >96%. Accumulation of the complexes in the focus of bacterial inflammation in bone structures and the absence of this complex in the site of aseptic inflammation was confirmed in a rat model of traumatic osteomyelitis by single-photon emission computed tomography.
Subject(s)
Osteomyelitis/drug therapy , Pore Forming Cytotoxic Proteins/chemistry , Ribosomal Proteins/chemistry , Technetium/chemistry , Animals , Diagnosis, Differential , Female , Rats , Rats, Wistar , Tomography, Emission-Computed, Single-PhotonABSTRACT
A model of traumatic osteomyelitis in rats is created. The disease development was evaluated by bone marrow myelograms, results of scintigraphy, and histological studies.
