[Determination of IgG content of IVIG by improved rocket immune electrophoresis].

OBJECTIVE: To establish a specific method for determination of IgG in IVIG. METHODS: The IVIG samples were disposed by acylation and analyzed by Rocket Immunoelectrophoresis. The amount of the IgG in IVIG was calculated by an IgG standard curve. RESULTS: The standard curve by this method has a very good linear relationship: r = 0.9967. The reproducibilities with 3 bathes of IVIG samples (n = 6) are 5.37% +/- 0.21%, 5.28% +/- 0.39% and 5.80% +/- 0.30%. CONCLUSION: This method could be adapted to regular assay in blood products.