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1.
Int J Mol Med ; 39(6): 1452-1460, 2017 Jun.
Article in English | MEDLINE | ID: mdl-28440421

ABSTRACT

Allicin is considered anti-atherosclerotic due to its antioxidant and anti-inflammatory effects, which makes it an important drug for the prevention and treatment of atherosclerosis. However, the effects of allicin on foam cells are unclear. Thus, in this study, we examined the effects of allicin on lipid accumulation via peroxisome proliferator-activated receptor Î³ (PPARγ)/liver X receptor α (LXRα) in THP­1 macrophage-derived foam cells. THP­1 cells were exposed to 100 nM phorbol myristate acetate (PMA) for 24 h, and then to oxydized low-density lipoprotein (ox-LDL; 50 mg/ml) to induce foam cell formation. The results of Oil Red O staining and high-performance liquid chromatography (HPLC) revealed showed that pre-treatment of the foam cells with allicin decreased total cholesterol, free cholesterol (FC) and cholesterol ester levels in cells, and also decreased lipid accumulation. Moreover, allicin upregulated ATP binding cassette transporter A1 (ABCA1) expression and promoted cholesterol efflux. However, these effects were significantly abolished by transfection with siRNA targeting ABCA1. Furthermore, PPARγ/LXRα signaling was activated by allicin treatment. The allicin-induced upregulation of ABCA1 expression was also abolished by PPARγ inhibitor (GW9662) and siRNA or LXRα siRNA co-treatment. Overall, our data demonstrate that the allicin-induced upregulation of ABCA1 promotes cholesterol efflux and reduces lipid accumulation via PPARγ/LXRα signaling in THP­1 macrophage-derived foam cells.


Subject(s)
ATP Binding Cassette Transporter 1/genetics , Foam Cells/drug effects , Liver X Receptors/metabolism , PPAR gamma/metabolism , Signal Transduction/drug effects , Sulfinic Acids/pharmacology , Up-Regulation/drug effects , ATP Binding Cassette Transporter 1/metabolism , Cell Line , Cholesterol/metabolism , Disulfides , Foam Cells/metabolism , Humans , Lipid Metabolism/drug effects , Macrophages/drug effects , Macrophages/metabolism , RNA, Messenger/genetics
2.
Med Sci Monit ; 23: 563-570, 2017 Jan 31.
Article in English | MEDLINE | ID: mdl-28139552

ABSTRACT

BACKGROUND The abnormal activity of Sirtuin 1 (Sirt1) is closely related to the aging of vascular endothelial cells. As a bioactive molecule, allicin has antioxidant, anti-inflammatory, and lipid-regulating mechanisms. However, few reports about the relationship of allicin and Sirt1 have been published. In this study, we aimed to elucidate the effect of allicin on Human Umbilical Vein Endothelial Cells (HUVECs) aging induced by hydrogen peroxide (H2O2) and the role of Sirt1 in this phenomenon. MATERIAL AND METHODS HUVEC were exposed to H2O2 to establish the aging model. The expression of protein and RNA were detected by Western blot and Reverse transcription-quantitative polymerase chain reaction. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to assess cell viability. Sirt1 enzyme activity assay was used to analyze enzymatic activity. Reactive oxygen species was detected by dichlorofluorescein diacetate (DCFH-DA). Cell aging was detected by Senescence ß-Galactosidase (SA-ß-gal) staining. RESULTS Results of this study revealed that pretreating HUVECs with 5 ng/mL allicin before exposure to H2O2 resulted in increased cell viability and reduced reactive oxygen species generation. Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that H2O2 attenuated the phosphorylation and activation of Sirt1 and increased the expression of plasminogen activator inhibitor-1(PAI-1) protein. Moreover, H2O2 also promoted HUVEC aging. These effects were significantly alleviated by 5 ng/mL allicin co-treatment. Furthermore, the anti-aging effects of allicin were abolished by the Sirt1 inhibitor nicotinamide (NAM). CONCLUSIONS Overall, the results demonstrated that allicin protects HUVECs from H2O2-induced oxidative stress and aging via the activation of Sirt1.


Subject(s)
Cellular Senescence/drug effects , Human Umbilical Vein Endothelial Cells/drug effects , Sirtuin 1/pharmacology , Sulfinic Acids/pharmacology , Antioxidants/pharmacology , Cell Survival/drug effects , Cells, Cultured , Disulfides , Drug Interactions , Endothelial Cells , Human Umbilical Vein Endothelial Cells/cytology , Humans , Hydrogen Peroxide/pharmacology , Niacinamide/pharmacology , Nitric Oxide/metabolism , Oxidative Stress/drug effects , Phosphorylation , Protective Agents/pharmacology , Reactive Oxygen Species/metabolism , beta-Galactosidase/metabolism
3.
Oncol Lett ; 11(2): 1499-1505, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26893768

ABSTRACT

The hepatocyte growth factor (HGF)/c-Met signaling pathway results in cancer cell scattering and invasion, and has been reported to participate in several types of cancer, including prostate and colorectal cancer. The downstream phosphorylation cascade of HGF, particularly the mitogen-activated protein kinase and phosphoinositide 3-kinase/AKT signaling pathway, regulates epithelial-mesenchymal transition (EMT). However, the mechanism by which these signaling pathways govern EMT, and whether certain kinases are able to respond to specific EMT effectors, remains to be elucidated. In the present study, an increase in the levels of vimentin, rather than co-regulation of certain EMT marker proteins, was observed in response to HGF-induced EMT in DU145 prostate cancer cells. In addition, it was observed that curcumin abrogated HGF-induced DU145 cell scattering and invasion. Furthermore, curcumin was able to effectively inhibit the HGF-induced increase in the levels of vimentin by downregulating the expression of phosphorylated c-Met, extracellular signal-regulated kinase and Snail. In conclusion, the results of the present study demonstrated that curcumin was able to reverse HGF-induced EMT, possibly by inhibiting c-Met expression in DU145 prostate cancer cells.

4.
DNA Cell Biol ; 34(9): 561-72, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26102194

ABSTRACT

Curcumin, a traditional Chinese derivative from the rhizomes of Curcuma longa, is beneficial to health by modulating lipid metabolism and suppressing atherogenesis. A key part of atherosclerosis is the failure of macrophages to restore their cellular cholesterol homeostasis and the formation of foam cells. In this study, results showed that curcumin dramatically increased the expression of ATP-binding cassette transporter 1 (ABCA1), promoted cholesterol efflux from THP-1 macrophage-derived foam cells, and reduced cellular cholesterol levels. Curcumin activated AMP-activated protein kinase (AMPK) and SIRT1, and then activated LXRα in THP-1 macrophage-derived foam cells. Inhibiting AMPK/SIRT1 activity by its specific inhibitor or by small interfering RNA could inhibit LXRα activation and abolish curcumin-induced ABCA1 expression and cholesterol efflux. Thus, curcumin enhanced cholesterol efflux by upregulating ABCA1 expression through activating AMPK-SIRT1-LXRα signaling in THP-1 macrophage-derived foam cells. This study describes a possible mechanism for understanding the antiatherogenic effects of curcumin on attenuating the progression of atherosclerosis.


Subject(s)
ATP Binding Cassette Transporter 1/metabolism , Cholesterol/metabolism , Curcumin/pharmacology , Foam Cells/metabolism , Hypolipidemic Agents/pharmacology , ATP Binding Cassette Transporter 1/genetics , Adenylate Kinase/metabolism , Cell Differentiation , Cell Line , Cell Survival/drug effects , Foam Cells/drug effects , Humans , Liver X Receptors , Orphan Nuclear Receptors/metabolism , Signal Transduction , Sirtuin 1/metabolism , Up-Regulation
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