[Effects of equol on proliferation of colorectal cancer HCT-15 cell].

OBJECTIVE: To investigate the effect of racemic equol and equol enantiomers on the proliferation of colorectal cancer HCT-15 cell and the potential mechanism. METHODS: 3-( 4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide( MTT) assay was used to detect the effect of different concentrations of racemic equol and equol enantiomers( 0, 0. 5, 1, 5 and 10 µmol/L) on the proliferation of colorectal cancer HCT-15 cell. Western blot was used to detect the expression of estrogen receptor( ER)and nuclear factor 2 related factor 2( Nrf2). RESULTS: Racemic equol and( R) equol inhibited the proliferation of HCT-15 cell in a dose-dependent manner, whereas( S) equol had no effect on the proliferation of HCT-15 cell. Racemic equol increased the expression of ERß and Nrf2, while( R) equol increased the expression of Nrf2. CONCLUSION: Racemic equol can inhibit the proliferation of HCT-15 cell through estrogenic and antioxidative activity. R equol can inhibit the proliferation of HCT-15 cell through antioxidative activity, while( S) equol has no effect on the proliferation of HCT-15 cell.

Dose-response relationship between body mass index and risks of all-cause mortality and disability among the elderly: A systematic review and meta-analysis.

BACKGROUND & AIMS: To establish the relationship between body mass index (BMI) and risks of mortality and disability among the Elderly. METHODS: A systematic review and dose-response meta-analysis was performed. PubMed, Embase, Cochrane library, and Google Scholar were searched systematically until December 2017 for relative studies reporting the hazard ratio (HR) and corresponding 95% confidence intervals (CIs) of all-cause mortality or disability across different BMI categories. RESULTS: 44 studies (37 studies on all-cause mortality and 9 studies on disability) were included in the meta-analysis. The restricted cubic spline model presents a U-shape trend, which suggests a relationship between BMI and all-cause mortality. As BMI increased, the all-cause mortality decreased from 1.49 (95% CI: 1.31, 1.71) to 0.96 (95% CI: 0.93, 0.98) in the 14.0-27.9 range and increased from 0.96 (95% CI: 0.94, 0.99) to 1.95 (95% CI: 1.37, 2.77) in the 28.0-47.9 range. In comparison with the reference BMI (23.0-23.9), the 24.0-29.0 BMI presented a significant protective effect, whereas <23.0 BMI and >33.0 BMI presented a significant risk effect on all-cause mortality. For disability, the restricted cubic spline model shows a nonlinear relationship. Individuals with >28.0 BMI and 33.0 BMI were 19% (95% CI: 1.01, 1.40) and 43% (95% CI: 1.13, 1.82) more prone to disability risks compared with those in the reference group, respectively. CONCLUSIONS: The lower-end recommended BMI range, underweight, and obesity among the elderly is associated with significantly increased risks of mortality and disability. The 23.0-28.0 BMI range may be the healthy weight range for the elderly group.